Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,977 T→C Q64Q (CAA→CAG)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,9770TC94.7% 111.5 / ‑3.5 40Q64Q (CAA→CAG) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (11/25);  minor base A (1/1);  total (14/26)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCG  >  CP006881/3724849‑3725110
                                                                                                                                |                                                                                                                                     
tgCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTGCTGCTATGGCGATTGCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTttct                                                                                                                              <  6:169587/137‑1 (MQ=255)
  cTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTcc                                                                                                                           <  3:170497/139‑1 (MQ=255)
         cTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                                                                                                    <  4:434895/139‑1 (MQ=255)
                       cctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTTCTTTTCTTGCTCATTGCCAGCCCTCCGCCag                                                                                                      >  8:112327/1‑138 (MQ=255)
                        ctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCat                                                                                                      >  7:238747/1‑137 (MQ=255)
                               ccGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATaaaa                                                                                              <  3:296563/139‑1 (MQ=255)
                                  ccATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGGGTTCTGCTTTTCTTCCTCATTCAAACCCATCCGCCATTCATAAAAcc                                                                                            >  3:394777/1‑137 (MQ=255)
                                     tGTAACGCTGCGGGCCCGCTCTCTCCTGCTGTGCCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGATTTTCTTCCACATTCACAGCCAGCCGCCATTCATAAAACGAAAc                                                                                        <  4:152602/139‑1 (MQ=255)
                                        aatgATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCAGAGCCATCCGCCATTCATAAAACGAAAcga                                                                                      <  3:475674/135‑3 (MQ=255)
                                            aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCGTTTCTTGCTCATTCACAGCCCTCCGCCATTCATAAAACGAAACGAGTcct                                                                                 >  6:185121/1‑138 (MQ=255)
                                               cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGGTTTGTGGTTTTGTTGCTCATTAACCCCCCGCCGCCCATCATAAAAAGAAACCAGTCCagcc                                                                              >  7:454951/1‑138 (MQ=255)
                                               cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                              >  8:375701/1‑139 (MQ=255)
                                               cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGCGTCCtgcg                                                                              >  1:36113/1‑139 (MQ=255)
                                                 ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                                                                                                    >  7:311767/1‑99 (MQ=255)
                                                 ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                                                                                                    <  8:311767/99‑1 (MQ=255)
                                                 ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc                                                                            >  7:992/1‑139 (MQ=255)
                                                 ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCGTTGTGGGTTTGTTCCGCATTCACAGCCCTCCGCCATTAATAAAACGAAACGAGTCCTGCGGc                                                                            >  7:109543/1‑139 (MQ=255)
                                                              cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                               <  5:223803/139‑2 (MQ=255)
                                                                 cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctt                                                             <  1:210621/138‑1 (MQ=255)
                                                                 cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTAGCTTTCTTCATTTGTTTCTCATTCACAGCCCTCCCCCATTGATAAAACGAAACCAGTACTGCCGCCTGCACcgccgccaat                                                            >  2:56740/1‑135 (MQ=255)
                                                                     ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTGCTGCGGCCTGCACAGCCGCCTTTCtt                                                         <  3:246301/138‑1 (MQ=255)
                                                                      tcGATTCCTTGAACGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGGATTATGCTTTTCATCCTCATTCACAGCCATCCGCCATTCAAAAATCGAAAGGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                        <  1:27840/137‑1 (MQ=255)
                                                                      gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCTTTATGGTTTTGTTACTCCTTAACAGCCCTACGCCATTAATAAAACCAAACGAGTCCTGCGGCCGGCCCCGCCGCCGTTCtttt                                                       >  8:219721/1‑138 (MQ=255)
                                                                      gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                                                                                                    <  5:442682/78‑1 (MQ=255)
                                                                      gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                                                                                                    >  6:442682/1‑78 (MQ=255)
                                                                      gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                        <  8:238747/138‑1 (MQ=255)
                                                                      gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                        <  2:79851/138‑1 (MQ=255)
                                                                           tCCTTGATGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                   <  1:183372/138‑1 (MQ=255)
                                                                                gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                              <  7:3706/138‑1 (MQ=255)
                                                                                 aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCGTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                            <  3:214660/139‑1 (MQ=255)
                                                                                 aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                            <  1:56740/139‑1 (MQ=255)
                                                                                    gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                         <  5:414688/139‑1 (MQ=255)
                                                                                    gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                         <  8:338682/139‑1 (MQ=255)
                                                                                      ttGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTTCGGCCAGCACAGCCGCCGTTCATTGGTATGTACGCTGAGcc                                       >  1:22755/1‑139 (MQ=255)
                                                                                                        ataAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCCGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                      <  7:375701/138‑1 (MQ=255)
                                                                                                         taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                    <  4:394777/139‑1 (MQ=255)
                                                                                                                 ggcggcTTTCGCTTGCTGGTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACCAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt            <  6:6162/139‑1 (MQ=255)
                                                                                                                     gctttcgctttcTGCTTTTATTCATCATTCACCGCCATCCGCATTTAAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGt        <  2:455046/139‑1 (MQ=255)
                                                                                                                      cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc       <  8:109543/139‑1 (MQ=255)
                                                                                                                      cTTTCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc       <  4:229669/139‑1 (MQ=255)
                                                                                                                           gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg  <  1:38708/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                     
AGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCG  >  CP006881/3724849‑3725110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑