Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,031	C→T	A46A (GCG→GCA)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,031	0	C	T	100.0%	112.9 / NA	36	A46A (GCG→GCA)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/30);  total (6/30)

CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAA  >  CP006881/3724896‑3725160                                                                                                                                        |                                                                                                                                  cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGGTTTGTGGTTTTGTTGCTCATTAACCCCCCGCCGCCCATCATAAAAAGAAACCAGTCCagcc                                                                                                                                >  7:454951/1‑138 (MQ=255) cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg                                                                                                                                >  8:375701/1‑139 (MQ=255) cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGCGTCCtgcg                                                                                                                                >  1:36113/1‑139 (MQ=255)   ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc                                                                                                                              >  7:992/1‑139 (MQ=255)   ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCGTTGTGGGTTTGTTCCGCATTCACAGCCCTCCGCCATTAATAAAACGAAACGAGTCCTGCGGc                                                                                                                              >  7:109543/1‑139 (MQ=255)                cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                 <  5:223803/139‑2 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctt                                                                                                               <  1:210621/138‑1 (MQ=255)                   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTAGCTTTCTTCATTTGTTTCTCATTCACAGCCCTCCCCCATTGATAAAACGAAACCAGTACTGCCGCCTGCACcgccgccaat                                                                                                              >  2:56740/1‑135 (MQ=255)                       ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTGCTGCGGCCTGCACAGCCGCCTTTCtt                                                                                                           <  3:246301/138‑1 (MQ=255)                        tcGATTCCTTGAACGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGGATTATGCTTTTCATCCTCATTCACAGCCATCCGCCATTCAAAAATCGAAAGGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                          <  1:27840/137‑1 (MQ=255)                        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCTTTATGGTTTTGTTACTCCTTAACAGCCCTACGCCATTAATAAAACCAAACGAGTCCTGCGGCCGGCCCCGCCGCCGTTCtttt                                                                                                         >  8:219721/1‑138 (MQ=255)                        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                          <  2:79851/138‑1 (MQ=255)                        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                          <  8:238747/138‑1 (MQ=255)                             tCCTTGATGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                     <  1:183372/138‑1 (MQ=255)                                  gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                <  7:3706/138‑1 (MQ=255)                                   aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCGTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                              <  3:214660/139‑1 (MQ=255)                                   aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                              <  1:56740/139‑1 (MQ=255)                                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                           <  8:338682/139‑1 (MQ=255)                                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                           <  5:414688/139‑1 (MQ=255)                                        ttGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTTCGGCCAGCACAGCCGCCGTTCATTGGTATGTACGCTGAGcc                                                                                         >  1:22755/1‑139 (MQ=255)                                                          ataAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCCGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                        <  7:375701/138‑1 (MQ=255)                                                           taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                      <  4:394777/139‑1 (MQ=255)                                                                   ggcggcTTTCGCTTGCTGGTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACCAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt                                                              <  6:6162/139‑1 (MQ=255)                                                                       gctttcgctttcTGCTTTTATTCATCATTCACCGCCATCCGCATTTAAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGt                                                          <  2:455046/139‑1 (MQ=255)                                                                        cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                         <  8:109543/139‑1 (MQ=255)                                                                        cTTTCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                         <  4:229669/139‑1 (MQ=255)                                                                             gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                    <  1:38708/139‑1 (MQ=255)                                                                                     cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                               <  1:83989/135‑1 (MQ=255)                                                                                                   tCACAGCCCTCCGGCATTCATAAAACGAAACGAGTCCTGCGGGCTGCTCTGCCGCCTTTCTTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTGCGGCGTCTCCCAGTTCATCTATAAgt                               <  7:35917/138‑2 (MQ=255)                                                                                                       aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATc                          <  5:183214/139‑1 (MQ=255)                                                                                                         ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                        <  7:219721/139‑1 (MQ=255)                                                                                                         ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                        <  1:212717/139‑1 (MQ=255)                                                                                                         acATCCGCAATTCATCACACGAAACGCGGCCTGCGGCCTGCACCGCCGCCTGTCTTTGGGATGTCCGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCAGTCCGGCGTCACCCAGTGCATCAAAAAGACCATCca                        <  4:335224/138‑1 (MQ=255)                                                                                                           aTCCGCCAGTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                      <  6:38762/139‑1 (MQ=255)                                                                                                            tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCacac                     <  8:424069/139‑1 (MQ=255)                                                                                                                 cagtCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                <  7:422303/136‑1 (MQ=255)                                                                                                                       tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa          <  8:454951/139‑1 (MQ=255)                                                                                                                            cGATACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc     <  3:483331/139‑1 (MQ=255)                                                                                                                               aaCGAGTCCTGCGGCCTGCACAGCCGCCGGTCTTTGGTATGTTCGCTGAGCCGCATAGACCGCGGCCGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTGATCAAAAAGACCATCCCCACCCGGAAAGATACAAGCaaa  >  5:218503/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                  CGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAA  >  CP006881/3724896‑3725160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑