Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,725,043 C→A A42A (GCG→GCT)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0430CA94.1% 110.3 / ‑6.5 35A42A (GCG→GCT) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (3/29);  total (4/31)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAA  >  CP006881/3724911‑3725175
                                                                                                                                    |                                                                                                                                    
cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                                <  5:223803/139‑2 (MQ=255)
   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctt                                                                                                                              <  1:210621/138‑1 (MQ=255)
   cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTAGCTTTCTTCATTTGTTTCTCATTCACAGCCCTCCCCCATTGATAAAACGAAACCAGTACTGCCGCCTGCACcgccgccaat                                                                                                                             >  2:56740/1‑135 (MQ=255)
       ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTGCTGCGGCCTGCACAGCCGCCTTTCtt                                                                                                                          <  3:246301/138‑1 (MQ=255)
        tcGATTCCTTGAACGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGGATTATGCTTTTCATCCTCATTCACAGCCATCCGCCATTCAAAAATCGAAAGGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                         <  1:27840/137‑1 (MQ=255)
        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCTTTATGGTTTTGTTACTCCTTAACAGCCCTACGCCATTAATAAAACCAAACGAGTCCTGCGGCCGGCCCCGCCGCCGTTCtttt                                                                                                                        >  8:219721/1‑138 (MQ=255)
        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                         <  8:238747/138‑1 (MQ=255)
        gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                         <  2:79851/138‑1 (MQ=255)
             tCCTTGATGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                                    <  1:183372/138‑1 (MQ=255)
                  gAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACg                                                                                                               <  7:3706/138‑1 (MQ=255)
                   aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCGTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                             <  3:214660/139‑1 (MQ=255)
                   aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                             <  1:56740/139‑1 (MQ=255)
                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                          <  5:414688/139‑1 (MQ=255)
                      gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                          <  8:338682/139‑1 (MQ=255)
                        ttGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTTCGGCCAGCACAGCCGCCGTTCATTGGTATGTACGCTGAGcc                                                                                                        >  1:22755/1‑139 (MQ=255)
                                          ataAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCCGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGc                                                                                       <  7:375701/138‑1 (MQ=255)
                                           taAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCg                                                                                     <  4:394777/139‑1 (MQ=255)
                                                   ggcggcTTTCGCTTGCTGGTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACCAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGttttt                                                                             <  6:6162/139‑1 (MQ=255)
                                                       gctttcgctttcTGCTTTTATTCATCATTCACCGCCATCCGCATTTAAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGt                                                                         <  2:455046/139‑1 (MQ=255)
                                                        cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                        <  8:109543/139‑1 (MQ=255)
                                                        cTTTCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc                                                                        <  4:229669/139‑1 (MQ=255)
                                                             gCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg                                                                   <  1:38708/139‑1 (MQ=255)
                                                                     cttttCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCAcc                                                              <  1:83989/135‑1 (MQ=255)
                                                                                   tCACAGCCCTCCGGCATTCATAAAACGAAACGAGTCCTGCGGGCTGCTCTGCCGCCTTTCTTTGGTTTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTGCGGCGTCTCCCAGTTCATCTATAAgt                                              <  7:35917/138‑2 (MQ=255)
                                                                                       aGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATc                                         <  5:183214/139‑1 (MQ=255)
                                                                                         ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                       <  7:219721/139‑1 (MQ=255)
                                                                                         ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                       <  1:212717/139‑1 (MQ=255)
                                                                                         acATCCGCAATTCATCACACGAAACGCGGCCTGCGGCCTGCACCGCCGCCTGTCTTTGGGATGTCCGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCAGTCCGGCGTCACCCAGTGCATCAAAAAGACCATCca                                       <  4:335224/138‑1 (MQ=255)
                                                                                           aTCCGCCAGTCAAAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCcaca                                     <  6:38762/139‑1 (MQ=255)
                                                                                            tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCacac                                    <  8:424069/139‑1 (MQ=255)
                                                                                                 cagtCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                               <  7:422303/136‑1 (MQ=255)
                                                                                                       tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa                         <  8:454951/139‑1 (MQ=255)
                                                                                                            cGATACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGc                    <  3:483331/139‑1 (MQ=255)
                                                                                                               aaCGAGTCCTGCGGCCTGCACAGCCGCCGGTCTTTGGTATGTTCGCTGAGCCGCATAGACCGCGGCCGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTGATCAAAAAGACCATCCCCACCCGGAAAGATACAAGCaaa                 >  5:218503/1‑139 (MQ=255)
                                                                                                                    ggcctgcgGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGttt            <  5:325873/134‑1 (MQ=255)
                                                                                                                       ctgcgGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCa          <  3:313106/136‑1 (MQ=255)
                                                                                                                               tGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCCGTTTTTCTGTCCGGCGTGCCCCAGTTCATCAAAAAGACCATCCACCCCCGGAAAGATACAAGCAAACGTTTCATAGaaaaa  >  3:408394/1‑138 (MQ=255)
                                                                                                                                    |                                                                                                                                    
CTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAA  >  CP006881/3724911‑3725175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑