Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,564,138	Δ1 bp	5.1%	coding (180/870 nt)	U712_13180 ←	putative HTH‑type transcriptional regulator yraN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,564,138	0	T	.	5.1%	269.4 / 6.2	78	coding (180/870 nt)	U712_13180	putative HTH‑type transcriptional regulator yraN
Reads supporting (aligned to +/- strand):  ref base T (35/39);  new base . (2/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTCTGCTGTTTTTGTTACACTTTG  >  CP006881/2564009‑2564272                                                                                                                                  |                                                                                                                                       ttGAGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGt                                                                                                                                <  2:96944/138‑1 (MQ=255) ttGAGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               <  6:275320/139‑1 (MQ=255) ttGAGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               <  3:326503/139‑1 (MQ=255) ttGAGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               <  6:368997/139‑1 (MQ=255)    aGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAg                                                                                                                            >  5:267196/1‑139 (MQ=255)    aGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAg                                                                                                                            >  2:368821/1‑139 (MQ=255)    aGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAg                                                                                                                            >  2:362897/1‑139 (MQ=255)      cGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               >  7:24582/1‑134 (MQ=255)      cGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               <  8:24582/134‑1 (MQ=255)      cGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAgg                                                                                                                           >  7:344239/1‑138 (MQ=255)       gAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGATGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGt                                                                                                                          >  2:154565/1‑138 (MQ=255)        aaaTTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  1:187300/139‑1 (MQ=255)        aaaTTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  1:310979/139‑1 (MQ=255)        aaaTTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  5:356834/139‑1 (MQ=255)        aaaTTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  6:170522/139‑1 (MQ=255)        aaaTTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  8:393676/139‑1 (MQ=255)                 gATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCttt                                                                                                                 <  1:99533/137‑1 (MQ=255)                 gATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAAATCCttt                                                                                                                 <  1:368821/137‑1 (MQ=255)                 gATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAAATCCttt                                                                                                                 <  1:362897/137‑1 (MQ=255)                  aTACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTAt                                                                                                               <  7:241034/138‑1 (MQ=255)                        ccAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCtttt                                                                                                        >  1:70556/1‑139 (MQ=255)                           gCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGt                                                                                                     >  6:159017/1‑139 (MQ=255)                                aGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               >  4:224099/1‑108 (MQ=255)                                aGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               <  3:224099/108‑1 (MQ=255)                                aGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGaa                                                                                                  <  4:168945/137‑1 (MQ=255)                                    gTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  3:269014/111‑1 (MQ=255)                                    gTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        >  4:269014/1‑111 (MQ=255)                                      ccTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATaattcaa                                                                                          >  5:198595/1‑139 (MQ=255)                                      ccTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATaattcaa                                                                                          >  7:143408/1‑139 (MQ=255)                                      ccTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTAGGTATCTTGAAGTTCCTGTAGCATACTTCTTGCATAAGCAACCAAGTGTACTCCTTACGCAGTAAAGGTAATTCCTTAATGCCTTTTGGTGAATaattca                                                                                           >  7:38372/1‑138 (MQ=255)                                       cTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATaattcaat                                                                                         >  7:297731/1‑139 (MQ=255)                                       cTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATaattcaat                                                                                         >  6:71812/1‑139 (MQ=255)                                         tGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATACTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGAAAAGGTAATTCCTTTAGGCCTTTTGGTGAATaattcaattc                                                                                       >  2:431393/1‑139 (MQ=255)                                          gCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCATTTATGCCTTTTGGTGAATAATTCAATTcc                                                                                      >  3:378690/1‑139 (MQ=255)                                                ctTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               >  1:348346/1‑92 (MQ=255)                                                ctTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTa                                                                                                                               <  2:348346/92‑1 (MQ=255)                                                 tttGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAATGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAAtc                                                                               >  3:336633/1‑139 (MQ=255)                                                   tGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAAt                                                                                <  8:297731/136‑1 (MQ=255)                                                     cTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATctcct                                                                           <  5:91749/139‑1 (MQ=255)                                                        agattagaGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCaa                                                                        >  5:406533/1‑139 (MQ=255)                                                               agaTATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  2:56448/84‑1 (MQ=255)                                                               agaTATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        >  1:56448/1‑84 (MQ=255)                                                               agaTATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCttttt                                                                 >  1:406966/1‑139 (MQ=255)                                                               agaTATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCtttt                                                                  >  7:307947/1‑138 (MQ=255)                                                                 atatGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCa                                                               <  6:198595/139‑1 (MQ=255)                                                                    tGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCTGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAAcc                                                            >  5:128397/1‑139 (MQ=255)                                                                    tGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAAcc                                                            >  8:75167/1‑139 (MQ=255)                                                                    tGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAAcc                                                            >  1:184326/1‑139 (MQ=255)                                                                    tGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAAcc                                                            >  5:343462/1‑139 (MQ=255)                                                                    tGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAAcc                                                            >  4:27086/1‑139 (MQ=255)                                                                    tGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAAcc                                                            >  5:182508/1‑139 (MQ=255)                                                                      aTCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCC‑TCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGa                                                          >  6:272174/1‑138 (MQ=255)                                                                      aTCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCC‑TCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAAACGa                                                          >  6:266414/1‑138 (MQ=255)                                                                         tttGGTATCTTGAAGTTCCTGTAGAATTATTCTTGCATAAGAAAACAAGTGTTATCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACTGAt                                                         >  5:90605/1‑137 (MQ=255)                                                                           tGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAATTCTCCTCAATCTTTTTCAACCGATAAg                                                      <  1:419889/138‑1 (MQ=255)                                                                           tGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGAt                                                         <  3:55428/135‑1 (MQ=255)                                                                           tGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGAt                                                         <  4:228494/135‑1 (MQ=255)                                                                           tGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATaatt                                                     >  3:228494/1‑137 (MQ=255)                                                                           tGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAg                                                      <  8:307947/138‑1 (MQ=255)                                                                           tGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAg                                                      <  2:406966/138‑1 (MQ=255)                                                                           tGGTATCATGAAGGTCCTGTATCATTCTTCTTGCATAAGCAAACAAGTGTTCTCCTTACGCAGTAAAGGTAATTACTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATa                                                        <  4:267795/136‑1 (MQ=255)                                                                                      aGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCt                                          <  5:207674/139‑1 (MQ=255)                                                                                      aGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCAGCAGTAAAGGTAATTCCTTTATGCCTTTTCGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCt                                          <  5:7467/139‑1 (MQ=255)                                                                                          ccTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCtgtg                                       <  6:182508/138‑1 (MQ=255)                                                                                           cTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGAt                                     <  6:267196/139‑1 (MQ=255)                                                                                                   ttcttcTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        <  2:46801/48‑1 (MQ=255)                                                                                                   ttcttcTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTaa                                                                                                                        >  1:46801/1‑48 (MQ=255)                                                                                                   ttcttcTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAaataa                             <  4:205276/139‑1 (MQ=255)                                                                                                           gCATAAGCAACCAAGTGTTCTCC‑TCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTc                       <  5:272174/136‑1 (MQ=255)                                                                                                           gCATAAGCAACCAAGTGTTCTCC‑TCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTc                       <  5:266414/136‑1 (MQ=255)                                                                                                                aGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTCTGCTGt                 <  6:406533/138‑1 (MQ=255)                                                                                                                aGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACAGATAAGTGATGGAAGGCTGTGATGTAAATAATCTTACTGCTGtt                >  2:398751/1‑139 (MQ=255)                                                                                                                 gCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTCTGCTGttt               <  8:411139/139‑1 (MQ=255)                                                                                                                      cAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTCTGCTGTTTTTGtt          <  4:119323/139‑1 (MQ=255)                                                                                                                      cAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTCTGCTGTTTTTGtt          <  3:27086/139‑1 (MQ=255)                                                                                                                      cAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTATGATGTAAATAATCGTTCTGCTGTTTTTGtt          <  8:344239/139‑1 (MQ=255)                                                                                                                            ttCTCCTTCCGCAGTAAAGGTAATTCCTATATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGAGAAGTGATGGAAGGCTGTGCTGTAAATAATCGTGCTGCTGTTTTTGGTacacgt    >  7:338784/1‑137 (MQ=255)                                                                                                                              ctcCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTCTGCTGTTTTTGTTACACTTTg  >  7:226708/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                       TTGAGCGAAATTGCTTGATACCCCCAGCCTTAGATGTCCTTGCACCTCTTTGCTTAGATTAGAGATATGATCTTTGGTATCTTGAAGTTCCTGTAGCATTCTTCTTGCATAAGCAACCAAGTGTTCTCCTTCCGCAGTAAAGGTAATTCCTTTATGCCTTTTGGTGAATAATTCAATTCCGAAAATCTCCTCAATCTTTTTCAACCGATAAGTGATGGAAGGCTGTGATGTAAATAATCGTTCTGCTGTTTTTGTTACACTTTG  >  CP006881/2564009‑2564272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑