Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,190,844	Δ1 bp	5.9%	coding (659/909 nt)	U712_16415 ←	Proline dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,190,844	0	G	.	5.9%	221.2 / 8.2	68	coding (659/909 nt)	U712_16415	Proline dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base G (26/38);  new base . (2/2);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

TAGCCTTCCTTGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTACAAGTCTGAGATTCGGCTTT  >  CP006881/3190709‑3190981                                                                                                                                        |                                                                                                                                          tAGCCTTCCTTGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        <  8:9112/139‑1 (MQ=255) tAGCCTTCCTTGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        <  7:282538/139‑1 (MQ=255) tAGCCTTCCTTGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        <  6:50262/139‑1 (MQ=255) tAGCCTTCCTTGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        <  1:422496/139‑1 (MQ=255) tAGCCTTCCTTGGCGAGTTCCTTCTGACGCTCCCGCCTCATGCCGTATACCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        >  5:336446/1‑139 (MQ=255)    ccttccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAg                                                                                                                                     <  1:293028/139‑1 (MQ=255)    ccttccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAg                                                                                                                                     <  2:221367/139‑1 (MQ=255)       tccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        >  1:344673/1‑133 (MQ=255)       tccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        <  2:344673/133‑1 (MQ=255)       tccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACTGCCg                                                                                                                                  >  4:240682/1‑139 (MQ=255)       tccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCg                                                                                                                                  >  1:181403/1‑139 (MQ=255)       tccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCg                                                                                                                                  >  1:138133/1‑139 (MQ=255)       tccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTG‑‑GACAGCCgtg                                                                                                                                >  3:228983/1‑139 (MQ=255)       tccttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTG‑‑GACAGCCgtg                                                                                                                                >  3:234451/1‑139 (MQ=255)         cttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCgtg                                                                                                                                >  5:397019/1‑139 (MQ=255)         cttGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGAAATGATGTCATCGTCATGTGTGGCGACAGCCgtg                                                                                                                                <  4:256486/139‑1 (MQ=255)                aGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTcc                                                                                                                         <  6:228358/139‑1 (MQ=255)                  ttCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGTCGACGAGCTGTTTCTTAAATGCAATGATGTCATCGTCATGTGTGGCTACAGCCTTGTAATTACCg                                                                                                                       >  2:443255/1‑139 (MQ=255)                  ttCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCg                                                                                                                       >  5:175069/1‑139 (MQ=255)                  ttCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCg                                                                                                                       <  7:16228/139‑1 (MQ=255)                     cTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCtt                                                                                                                    <  4:176626/139‑1 (MQ=255)                             gCTCCGGCCTGCTGCCGTAAAGCAGTCGAACCGCAAAATGCGAAGCGGGGATTAGATGTTCGGCGACGAGCTGTTGCGTAAATGCAATGATGTCCTCGGCATGTGGGGCGACAGCCGGGGAGATCCCGCTTAACCACTg                                                                                                            <  4:143017/139‑1 (MQ=255)                               tCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        <  6:254670/109‑1 (MQ=255)                               tCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        >  5:254670/1‑109 (MQ=255)                               tCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCa                                                                                                          >  1:214497/1‑139 (MQ=255)                                 cGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAg                                                                                                         <  6:303526/138‑1 (MQ=255)                                     cTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTG‑‑GACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTttatt                                                                                                  <  4:228983/139‑1 (MQ=255)                                     cTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTG‑‑GACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTttatt                                                                                                  <  4:234451/139‑1 (MQ=255)                                       gATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGCGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTACCACCTGCCGCTtgatt                                                                                                  >  7:232041/1‑139 (MQ=255)                                        aTGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTttatt                                                                                                  >  8:159316/1‑138 (MQ=255)                                         tGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTttatt                                                                                                  >  7:415683/1‑137 (MQ=255)                                         tGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTttatt                                                                                                  >  8:86129/1‑137 (MQ=255)                                              tataGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTATATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCTCTTAACAACTGCAGCTTTATTAAGctct                                                                                           >  6:126802/1‑139 (MQ=255)                                              tataGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGctct                                                                                           >  5:155890/1‑139 (MQ=255)                                              tataGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGctct                                                                                           >  8:231213/1‑139 (MQ=255)                                                taGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGa                                                                                         <  8:415683/139‑1 (MQ=255)                                                taGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGa                                                                                         <  1:443255/139‑1 (MQ=255)                                                taGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGa                                                                                         <  4:222002/139‑1 (MQ=255)                                                     tttGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAAt                                                                                     >  3:220478/1‑138 (MQ=255)                                                      ttGAAACTCAAATTGAGATGCGGTGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGAAAGCCGTGTATTTACCGCTTTACAACTGCAGCTTTATTCATCTCTGAAAAta                                                                                    >  8:114475/1‑137 (MQ=255)                                                        gataCTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        <  6:221065/81‑1 (MQ=255)                                                        gAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTACGTAAATGCAATGATGTCATCGTCATGTGTGGCGa                                                                                                                                        >  5:221065/1‑84 (MQ=255)                                                               taaTTGCGATGCGGTGAGTTGATGTTCGGCGACGAGCTGTTTCCTAAATGCTATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCTGCTTTATTAAGCTCTGAAAATGAAGGTCCg                                                                            <  6:336446/136‑1 (MQ=255)                                                                       aTGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCtttt                                                                  >  3:225772/1‑139 (MQ=255)                                                                           ggggATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCg                                                              <  4:314426/139‑1 (MQ=255)                                                                           ggggATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCg                                                              <  6:437062/139‑1 (MQ=255)                                                                           ggggATTTGATGATCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTAATAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCg                                                              <  6:265152/139‑1 (MQ=255)                                                                              gATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGa                                                           <  2:214497/139‑1 (MQ=255)                                                                              gATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGa                                                           <  3:446492/139‑1 (MQ=255)                                                                              gATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGGTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGa                                                           <  8:334696/139‑1 (MQ=255)                                                                                     tGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTa                                                    <  7:159316/139‑1 (MQ=255)                                                                                               cgaGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGa                                          <  7:231213/139‑1 (MQ=255)                                                                                               cgaGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGa                                          <  2:181403/139‑1 (MQ=255)                                                                                               cgaGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGa                                          <  4:220478/139‑1 (MQ=255)                                                                                                         cGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTtat                                  >  1:60853/1‑137 (MQ=255)                                                                                                          ggaactGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGCAGGTCCGGGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAg                               <  8:183852/134‑1 (MQ=255)                                                                                                            aaaTGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGGTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACATCCGCCGATTATTTAAAAgcg                             >  4:277959/1‑139 (MQ=255)                                                                                                             aaTGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAgcg                             <  6:175069/138‑1 (MQ=255)                                                                                                                gCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTTAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGGTTGTTTTTAATCGCCtg                         >  1:219987/1‑138 (MQ=255)                                                                                                                   atgatgTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTa                      <  4:252567/139‑1 (MQ=255)                                                                                                                    tgcagTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCATTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTNTTAc                     <  6:305509/135‑1 (MQ=255)                                                                                                                    tgatgTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTAc                     <  7:388899/139‑1 (MQ=255)                                                                                                                         tCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTACAAGTc                <  2:12746/139‑1 (MQ=255)                                                                                                                           aTCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAgcg                             >  3:233831/1‑124 (MQ=255)                                                                                                                           aTCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAgcg                             <  4:233831/124‑1 (MQ=255)                                                                                                                                 aTGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAg                                                                                                         <  7:85745/42‑1 (MQ=255)                                                                                                                                 aTGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAg                                                                                                         >  8:85745/1‑42 (MQ=255)                                                                                                                                 aTGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTACAAGTCTGAGAtt         <  7:86129/138‑1 (MQ=255)                                                                                                                                       ggCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTACAAGTCTGAGATTCGGCttt  <  7:194501/139‑1 (MQ=255)                                                                                                                                        gCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTACAAGTCTGAGATTCGGCttt  <  3:240682/138‑1 (MQ=255)                                                                                                                                        gCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTACAAGTCTGAGATTCGGCttt  <  4:414305/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          TAGCCTTCCTTGGCGAGTTCCTTCTGACGCTCCGGCCTGATGCCGTATAGCATTTGAAACTCAAATTGCGATGCGGGGATTTGATGTTCGGCGACGAGCTGTTTCGTAAATGCAATGATGTCATCGTCATGTGTGGCGACAGCCGTGTAGTTCCCGCTTAACAACTGCAGCTTTATTAAGCTCTGAAAATGAAGGTCCGTGCCTCTTTTGTCGGGAAACGCTACAGCCGCAGATTCTTTATAAGCGCCTTTTACAAGTCTGAGATTCGGCTTT  >  CP006881/3190709‑3190981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑