Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,285,257:1	+T	100%	intergenic (‑77/+143)	U712_16905 ← / ← U712_16910	Transcriptional repressor sdpR/Glycine betaine/carnitine/choline transport system permease protein opuCD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,285,257	1	.	T	92.6%	225.5 / 10.3	68	intergenic (‑77/+143)	U712_16905/U712_16910	Transcriptional repressor sdpR/Glycine betaine/carnitine/choline transport system permease protein opuCD
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base T (33/30);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

ATCCAAAATTTTTCTCCTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAA‑TT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTAAAAAAACCACCTCTGT  >  CP006881/3285127‑3285394                                                                                                                                    |                                                                                                                                           aTCCAAAATTTTTCTCCTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAA‑tt‑ttttt                                                                                                                                    >  1:386154/1‑138 (MQ=255) aTCCAAAATTTTTCTCCTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAA‑tt‑ttttt                                                                                                                                    >  2:304348/1‑138 (MQ=255)       aaTTTTTCTCCTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAA‑TT‑TTTTTtaaaaac                                                                                                                             <  5:390585/139‑3 (MQ=255)       aaTTTTTCTCCTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAA‑TT‑TTTTTtaaaaac                                                                                                                             <  5:396934/139‑3 (MQ=255)                 cTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCaaa                                                                                                                    <  7:364194/139‑1 (MQ=255)                 cTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCaaa                                                                                                                    <  6:275318/139‑1 (MQ=255)                    gttgGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAAt                                                                                                                   <  1:216664/137‑1 (MQ=255)                    gttgGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAAt                                                                                                                   <  8:210161/137‑1 (MQ=255)                          tCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTtat                                                                                                            >  6:160927/1‑138 (MQ=255)                                 taGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCt                                                                                                     <  7:332759/138‑1 (MQ=255)                                    cTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGAtt                                                                                                 >  8:42569/1‑139 (MQ=255)                                       tGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGAttttt                                                                                              >  2:73511/1‑139 (MQ=255)                                        gAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTg                                                                                             >  7:15309/1‑139 (MQ=255)                                        gAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTg                                                                                             >  2:71865/1‑139 (MQ=255)                                        gAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTg                                                                                             >  8:161112/1‑139 (MQ=255)                                              attattCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTAtttt                                                                                       >  7:175533/1‑139 (MQ=255)                                              attattCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTAtttt                                                                                       >  3:43924/1‑139 (MQ=255)                                                     aTAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCaaa                                                                                 >  5:134326/1‑138 (MQ=255)                                                     aTAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCaaa                                                                                 >  5:347464/1‑138 (MQ=255)                                                       aGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCa                                                                                   <  7:310906/134‑1 (MQ=255)                                                       aGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCa                                                                                   >  8:310906/1‑134 (MQ=255)                                                       aGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAACATCTGATTTTTGTATTTTTTCAAATAc                                                                              >  5:444842/1‑139 (MQ=255)                                                       aGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATAGGTTATAATATCTGATTTTTGTATTTTTTCAAATAc                                                                              >  8:98538/1‑139 (MQ=255)                                                       aGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAA‑TT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACt                                                                             >  1:227613/1‑139 (MQ=255)                                                            tttCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTAAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGa                                                                         >  7:390230/1‑139 (MQ=255)                                                              tctcCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGAt                                                                        <  8:362206/138‑1 (MQ=255)                                                              tctcCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGAt                                                                        <  2:370032/138‑1 (MQ=255)                                                              tctcCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGAt                                                                        <  6:105871/138‑1 (MQ=255)                                                                     tACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCaa                                                                >  4:117628/1‑139 (MQ=255)                                                                       cAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATATTCCCAACg                                                              <  6:314176/139‑1 (MQ=255)                                                                       cAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACg                                                              <  6:376007/139‑1 (MQ=255)                                                                         aaaaaaCATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCt                                                            <  6:415208/139‑1 (MQ=255)                                                                          aaaaaCATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTg                                                           >  7:68476/1‑139 (MQ=255)                                                                              acatttagacatttagATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGaaa                                                       <  7:42569/139‑1 (MQ=255)                                                                              acatttagacatttagATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGaaa                                                       <  1:73511/139‑1 (MQ=255)                                                                              acatttagacatatagATATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGaaa                                                       <  4:203308/139‑1 (MQ=255)                                                                                atttagacatttagaTATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAat                                                     <  7:98538/139‑1 (MQ=255)                                                                                atttagacatttagaTATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAat                                                     <  2:386154/139‑1 (MQ=255)                                                                                 tttagacatttagaTATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAGCGCTGGAAAata                                                    >  6:288207/1‑139 (MQ=255)                                                                                        atttagaTATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTaa                                             <  4:43924/139‑1 (MQ=255)                                                                                          ttagaTATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTaa                                             >  2:146357/1‑137 (MQ=255)                                                                                           tagaTATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACa                                           <  1:232532/138‑1 (MQ=255)                                                                                             gaTATTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGa                                         <  3:49160/138‑1 (MQ=255)                                                                                              atatTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATa                                       <  3:117628/139‑1 (MQ=255)                                                                                               tatTTGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATa                                       <  6:134326/138‑1 (MQ=255)                                                                                                 tttGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACt                                     <  7:388113/138‑1 (MQ=255)                                                                                                 tttGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACt                                     <  6:347464/138‑1 (MQ=255)                                                                                                  ttGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATACTCCCAACGATGGAAAATATGTTTAACAGATACTa                                    >  8:341107/1‑138 (MQ=255)                                                                                                  ttGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTaa                                   >  8:348048/1‑139 (MQ=255)                                                                                                  ttGTATAAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTaa                                   >  6:446704/1‑139 (MQ=255)                                                                                                  ttGTATAAATGTATTATTTTCATTATAACTTGAA‑TTCTTTTTAAAAACACCCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCACCGCTGGAAAATATGTTTACCAGATACTac                                   >  6:280582/1‑138 (MQ=255)                                                                                                  ttGTATAAATGTATTATTTTCATTATAACTTGAA‑TTCTTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTaa                                   >  6:274881/1‑139 (MQ=255)                                                                                                     tataAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAaca                                 >  7:273491/1‑138 (MQ=255)                                                                                                       taAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCaa                                                                <  7:158880/105‑1 (MQ=255)                                                                                                       taAATGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCaa                                                                >  8:158880/1‑105 (MQ=255)                                                                                                        aaaTGTATTATTTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACAtg                             >  2:285242/1‑139 (MQ=255)                                                                                                               ttattTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGttta                      >  8:438640/1‑139 (MQ=255)                                                                                                               ttattTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGttta                      >  6:241254/1‑139 (MQ=255)                                                                                                               ttattTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGttta                      >  3:184546/1‑139 (MQ=255)                                                                                                               ttattTTCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGttta                      >  8:432898/1‑139 (MQ=255)                                                                                                                    ttCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTAGtttattta                  >  4:154144/1‑138 (MQ=255)                                                                                                                     tCATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTaa                 >  5:130720/1‑138 (MQ=255)                                                                                                                      cATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTaaa                <  5:160927/138‑1 (MQ=255)                                                                                                                      cATTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTATAACAGATACTAACACATGTTGTTTATTTaaa                <  7:118722/138‑1 (MQ=255)                                                                                                                       aTTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTaaaat              <  5:409118/139‑2 (MQ=255)                                                                                                                       aTTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTaaaa               >  5:226849/1‑138 (MQ=255)                                                                                                                       aTTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTaaaa               >  8:285008/1‑138 (MQ=255)                                                                                                                       aTTATAACTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTaaaa               >  6:5730/1‑138 (MQ=255)                                                                                                                             aCTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTAAAAAAaccac        <  5:139588/139‑1 (MQ=255)                                                                                                                             aCTTGAATTT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTAAAAAAaccac        <  7:134858/139‑1 (MQ=255)                                                                                                                                   a‑TT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTAAAAAAACCACCTCtgt  <  2:227613/138‑1 (MQ=255)                                                                                                                                    |                                                                                                                                           ATCCAAAATTTTTCTCCTTGTTGGATCTGATATAGCTTTGAAAACATTATTCATAGCGTTTTCTCCTTTACAAAAAAACATTTAGACATTTAGATATTTGTATAAATGTATTATTTTCATTATAACTTGAA‑TT‑TTTTTAAAAACAACCCTCAAATTGGTTATAATATCTGATTTTTGTATTTTTTCAAATACTCGGATAATCCCAACGCTGGAAAATATGTTTAACAGATACTAACACATGTTGTTTATTTAAAAAAACCACCTCTGT  >  CP006881/3285127‑3285394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑