| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 389,046 | T→G | 17.1% | R437R (CGT→CGG) | U712_01810 → | Surfactin synthase subunit 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 389,046 | 0 | T | G | 17.1% | 51.4 / 2.2 | 43 | R437R (CGT→CGG) | U712_01810 | Surfactin synthase subunit 2 |
| Reads supporting (aligned to +/- strand): ref base T (12/23); new base G (6/2); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGCAAGCGAAACGGACGCCGGCGAGCACCG > CP006881/388934‑389182 | tCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGGGGGGTTACGGGCTTGTGGGAGCCGaa > 4:351564/1‑137 (MQ=255)tCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCGTTTGTGCGTGAGGTCGGGCGTTGGGGGGACGAagc > 7:34809/1‑138 (MQ=255) cAAAATAAAATATAACGGAGCCGCCTTTGATTCCGCTGTTATCGACCGGACAGCGGAGCACCATACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGGGAGTACGGGCTTGTGGGAGACGAagag < 2:105142/139‑1 (MQ=255) aaTATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCa < 3:222821/139‑1 (MQ=255) tataACGGAGCCGCCGTTGATTCCGCTTTTATCGAACGGACCGCGGAGCACCTAACCCGCATGATGGAAGCAGCTGGCGATCAGCCGGCCGCTTTTGTGCGGGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCaaa < 8:364562/139‑1 (MQ=255) cGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCGTTTTTGGGGGCGTATGGGGGTGGGGGGGGCGAAGCGGCGCGGGCAAAtttg > 1:232347/1‑137 (MQ=255) ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCg < 1:18836/139‑1 (MQ=255) ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCg < 8:300601/139‑1 (MQ=255) ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCg < 8:146736/139‑1 (MQ=255) ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCg < 4:430889/139‑1 (MQ=255) ggAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGGGGGAGACGAAGAGCAGC‑GGCAAATTGTCg < 8:301493/139‑1 (MQ=255) aGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAg < 3:236302/139‑1 (MQ=255) ccgccTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTGTTTTGCGTGAGTACCGGGGTGTGGGGGCCGAAGAGCCCC‑GGGAAAATGtgcaggg > 1:125262/1‑133 (MQ=255) ccgttGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCGGTCGATCAGCCGGCCGCGTTTTTGGGGGGGTTAGGGGGGGGGGGGGGCGAAGAGGCGC‑GGCGAATTGTCGAGGTGtt > 4:354409/4‑139 (MQ=255) ttGATTGCGCTTTTTTCGAACGGACAGCGGAGCACCGTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCGTTTTTGGGGGTGTTGGGGGGTGTTGGGGGCGCAGGGGCGC‑GGCCAAATTTGGAGGTGtttt > 6:376817/1‑137 (MQ=255) gcttCCGCTTTTCTCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAAc < 3:295595/136‑1 (MQ=255) ttCCGCTTTTATCGAACGGACAGCGGCGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCGTTTGTGCGGGTGTACGGGCGTGTGGGGGACGAAGAGCCGC‑GGCCAATTGGGGGGGGGTTTAACAGc > 8:326289/1‑139 (MQ=255) cGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTTCGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACg > 6:73789/1‑139 (MQ=255) ttATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGaaa < 5:73789/137‑1 (MQ=255) aTCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCGTTTGTGCGTGAGTAAGGGCTTGTGGGAGACGAAGAGCCGC‑GGCAAATTTTAGAGGTGTTTTACAGCACGAAAGCCg > 2:102232/1‑139 (MQ=255) cGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGaa < 3:80248/139‑1 (MQ=255) gACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCt < 1:322342/139‑1 (MQ=255) cGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTTTGGGTGAGTTAGGGCGTGGGGGGGTCGCAGAGCAGC‑GGCCAATTTTAGAGGGGTTTAAACTCACGAAAGCCGAACACCCAGAAgg > 4:80248/1‑139 (MQ=255) gAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGaa < 1:281812/139‑1 (MQ=255) aCCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGc < 3:215878/139‑1 (MQ=255) cTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGGGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTg < 5:6860/139‑1 (MQ=255) tACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCGTTTGTTGGTGAGTAAGGGCTTGTGGGTGTCGAAAAGCAGC‑GGCAAATTGTGGAGGTGGTTTAAAGCCCGGAACGCCAACAGCCTGAAGTAATGTCTGt > 4:433618/1‑138 (MQ=255) ccGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCAc < 3:354409/139‑1 (MQ=255) gCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTTTTCGTGAGTACGGGCTTTTGGGAGAAGAAGACCACC‑AGCAAAATTTAGAGGTGTTTAACAACACGAAATCCGAACTCCCTGAAGTAATTGCTTTTAAcc > 6:6860/1‑138 (MQ=255) gCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCa < 1:102232/139‑1 (MQ=255) gCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCa < 8:34809/139‑1 (MQ=255) gCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTAAGGGCTTGTGGGAGCCGAAGAGCAGC‑GGCAAAATGTCGAAGTGTTTTAAAGCACGCAAGCCGAACACCCAGAAGGAAAGGCGGttaccc > 4:309439/1‑134 (MQ=255) gCATGATGGAACCAGCTGGCGAGCAGCCGGCCGCTTTTGTGCGTGAGTCCGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCa < 6:197036/139‑1 (MQ=255) tgatgGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGGGGGAGTACGGGGGTGTGGGAGACGCAGAGCAGC‑GGCAAATTTTCGCGGTGTTTAACAGCACGAAAGCCGCACACCCGGAAGGGATGGCTGGTCACCAAgt > 4:296177/1‑139 (MQ=255) tgatgGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTa < 1:255327/46‑1 (MQ=255) tgatgGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTa > 2:255327/1‑46 (MQ=255) tgatgGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAagag < 7:348902/68‑1 (MQ=255) tgatgGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAagag > 8:348902/1‑68 (MQ=255) gcagcTGTCGATCAGCCGGCCGCGTTTGTGCGTGAGGACGGGGGTGTGGGTGGCGAAGAGCCGC‑GGCAAATTGTCGCGGTGTTTTACAGCACGAAAGCCGAACTCCCGGCAGGAATGGCTGTTCACCAAGGGGTTGGag > 2:293654/1‑139 (MQ=255) agcTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAATTTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAagag < 3:309439/139‑1 (MQ=255) gccggccgCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCt < 2:234201/94‑1 (MQ=255) gccggccgCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCt > 1:234201/1‑94 (MQ=255) cGTGAGTACGGGCTTGGGGGAGACGACGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGCAAGCGAAACGGACGCCGGCGAGCAccg < 7:326289/139‑1 (MQ=255) | TCAAAATTAAATATAACGGAGCCGCCTTTGATTCCGCTTTTATCGAACGGACAGCGGAGCACCTTACCCGCATGATGGAAGCAGCTGTCGATCAGCCGGCCGCTTTTGTGCGTGAGTACGGGCTTGTGGGAGACGAAGAGCAGC‑GGCAAATTGTCGAGGTGTTTAACAGCACGAAAGCCGAACTCCCTGAAGGAATGGCTGTTCACCAAGTGTTTGAAGAGCAAGCGAAACGGACGCCGGCGAGCACCG > CP006881/388934‑389182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |