Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,459	T→C	100%	T91T (ACT→ACC)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,459	0	T	C	100.0%	147.4 / NA	42	T91T (ACT→ACC)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/26);  total (16/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCT  >  CP006881/3723333‑3723594                                                                                                                               |                                                                                                                                        cAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACCCCTTTTATTTCACCTATACcgcgata                                                                                                                             >  8:220063/1‑136 (MQ=255)              aTGGCTGATTCTCCGATTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTAGGGTGATTGCTTACGGAGGCCGAGAATTATTTACAGGCACCACCATTTATTTGACCTATACCGCGCTCCGGAAAAAAAtc                                                                                                                 >  4:292073/1‑137 (MQ=255)                 gctgATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTTACGGCAACACCTTTTATTTCACCTATACCGCGCACCGGAAAAAAAtcat                                                                                                               >  8:171272/4‑134 (MQ=255)                     aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTGTGATGATTGCTTACGGAGGCGGAGATTTTTTTACCGGCAACCCCTTTTTTTTGACCTATACCGCGCTCCGGGAAAAAATCAGCTGGCg                                                                                                         >  8:185297/1‑139 (MQ=255)                     aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCg                                                                                                         >  5:39017/1‑139 (MQ=255)                        ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                                       <  3:79381/138‑3 (MQ=255)                        ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                                       <  3:176745/138‑3 (MQ=255)                        ctcCGTTTGCCGTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCGTACGGAGGCGGATATTTATTTACCGGCAACACCTTTTATTTCACCTATCCCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                      <  3:427095/139‑1 (MQ=255)                        ctcCGTTTGCATTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGCTTGCTTACGGAGGCGGAGAATTATTTACCGGCAACACCTTTTATTTCACCTATAGATCGATCCTTAAAAAAATCAGCTGGCgcg                                                                                                       <  7:185297/138‑3 (MQ=255)                          ccGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCGGGCGCGaca                                                                                                    <  7:182247/139‑1 (MQ=255)                          ccGCTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGAATTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAACGCAGCTGGCGCGaca                                                                                                    <  1:393523/139‑1 (MQ=255)                           cGTTTGCCTTTCCGGGAGCCTCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                      <  3:102375/136‑1 (MQ=255)                           cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                      <  7:220063/136‑1 (MQ=255)                           cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                      <  3:200192/136‑1 (MQ=255)                           cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                      <  3:113460/136‑1 (MQ=255)                           cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                                                    <  2:142898/138‑1 (MQ=255)                           cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                                                    <  8:39947/138‑1 (MQ=255)                           cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                                                    <  8:125476/138‑1 (MQ=255)                               tGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCGCCGGAAAAAAATCAGCTGGCGCGACACCCta                                                                                               >  7:186774/1‑138 (MQ=255)                                gcctttCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCta                                                                                               <  8:410239/135‑2 (MQ=255)                                       cGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgt                                                                                        <  3:4385/138‑1 (MQ=255)                                        ggCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTgg                                                                                      <  7:125730/139‑1 (MQ=255)                                           aGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATg                                                                                   <  8:259257/139‑1 (MQ=255)                                                                          atgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTTGCGCGCCCCCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTTATCGGCGCCa                                                    >  4:387850/2‑139 (MQ=255)                                                                          atgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACCCCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                                                    >  8:126424/2‑139 (MQ=255)                                                                          atgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                                                    >  5:343392/2‑139 (MQ=255)                                                                           tgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                                                    <  4:51983/138‑1 (MQ=255)                                                                              ttGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                                <  4:103047/139‑1 (MQ=255)                                                                               tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                      >  1:255487/1‑84 (MQ=255)                                                                               tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                      <  2:255487/84‑1 (MQ=255)                                                                               tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCtg                                               >  3:139671/1‑138 (MQ=255)                                                                               tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGATGGCGCGACACACTATACTTGTGGATGTCTAGCTATGCCGGCAATTAAATCGGCGCCATGCtg                                               >  1:379812/1‑138 (MQ=255)                                                                                 cTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGtt                                             >  1:285751/1‑139 (MQ=255)                                                                                    aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                           <  4:61397/138‑1 (MQ=255)                                                                                    aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                           <  8:102653/138‑1 (MQ=255)                                                                                     cGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGcc                                         <  3:292073/139‑1 (MQ=255)                                                                                                    atttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCg                                                          >  6:193037/1‑107 (MQ=255)                                                                                                    atttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCg                                                          <  5:193037/107‑1 (MQ=255)                                                                                                    atttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGcgcg                          >  4:316533/1‑139 (MQ=255)                                                                                                                      ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga         <  3:316533/138‑1 (MQ=255)                                                                                                                      ttATATCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga         <  6:198187/138‑1 (MQ=255)                                                                                                                            cACCTATACCGAGCTCCGGAAAAAAATCAGCTGGCGCGCCCCCCTATACTTGTGGATGTCAAGCTATGCCTGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGAGACGGGACTGTTTGAGGAGCCTTCt  >  4:370115/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                        CAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCT  >  CP006881/3723333‑3723594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑