Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	572,484	T→G	19.2%	V21V (GTT→GTG)	U712_02825 →	Putative membrane protein ydgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	572,484	0	T	G	19.2%	128.0 / 5.4	66	V21V (GTT→GTG)	U712_02825	Putative membrane protein ydgH
Reads supporting (aligned to +/- strand):  ref base T (6/46);  new base G (12/2);  total (18/48)
Fisher's exact test for biased strand distribution p-value = 2.76e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTGTA  >  CP006881/572349‑572619                                                                                                                                        |                                                                                                                                        aGGAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGtttt                                                                                                                                       >  8:149700/1‑138 (MQ=255) aGGAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGtgttt                                                                                                                                      >  8:204090/1‑138 (MQ=255)   gAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCATATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGttttttg                                                                                                                                    >  4:203029/1‑136 (MQ=255)   gAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTTAg                                                                                                                                    >  7:269472/1‑139 (MQ=255)   gAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAg                                                                                                                                    >  1:166584/1‑139 (MQ=255)   gAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAg                                                                                                                                    >  3:303526/1‑139 (MQ=255)   gAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTgtg                                                                                                                                    >  1:239969/1‑137 (MQ=255)   gAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTgtg                                                                                                                                    >  6:449075/1‑137 (MQ=255)   gAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTGAg                                                                                                                                    >  4:167279/1‑139 (MQ=255)    aaCACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAgt                                                                                                                                   <  2:91383/139‑1 (MQ=255)        cTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAgtttgt                                                                                                                               <  1:321659/139‑1 (MQ=255)                cTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTcc                                                                                                                        <  8:252931/138‑1 (MQ=255)                cTATCATCGAGATATTCAACATTTTAAAAAGCAAATTTTAAGGAGTAGATTCTGCCGAATTAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACGGTCGTTTTGAGTTTGTTTTCTcc                                                                                                                        <  8:430176/138‑1 (MQ=255)                   tcatcGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGTCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTGTGTTTGTTTTCTCCGAAc                                                                                                                    >  2:107988/1‑139 (MQ=255)                   tcatcGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGtt                                                                                                                                         >  5:181546/1‑118 (MQ=255)                   tcatcGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGtt                                                                                                                                         <  6:181546/118‑1 (MQ=255)                   tcatcGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGaa                                                                                                                     <  8:304831/138‑1 (MQ=255)                   tcatcGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTTTATTTTTTTTTTACGaaa                                                                                                                    >  2:321659/1‑138 (MQ=255)                   tcatcGAGATATTCAACATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGaa                                                                                                                     <  1:79280/138‑1 (MQ=255)                       cGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTGTGTTTGTTTTGTGCGAACTTaa                                                                                                                >  8:85772/1‑139 (MQ=255)                       cGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAAATGCAGCCATAGTATTAGCACTTACCGTCGTGTTGTTTTAGTTTTTTCCCaaagaa                                                                                                                 >  4:77763/1‑134 (MQ=255)                        gagaTATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTaa                                                                                                                <  4:199451/138‑1 (MQ=255)                           atatTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTGCGAAcgttaag                                                                                                              >  4:297489/1‑131 (MQ=255)                           atatTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTTAGTTTTTTTTAACCCAACATAAccag                                                                                                            >  6:230104/1‑136 (MQ=255)                            tatTCACCATTTTAATAAGCAAATTTAAAGGAGGAGATTCTGCAGAAAGAGAGCAATCATCAAATTCAAGTGGGCAATAGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAg                                                                                                           <  1:184687/139‑1 (MQ=255)                               tCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGGTTTGTTTGTTTTTTGCGGACAGAAAGGAGCTg                                                                                                        >  3:172075/1‑139 (MQ=255)                                               cAAATTTAAAGGAGGAGATTCAGCCGAATGAGAGCAATCATCAAATTCTAGTGGTCAATTGCAGCCATAGTATTAGCACTTTCCGTCTTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                        <  6:265792/139‑1 (MQ=255)                                                     tacaggaggAGCTTCTGCCGAACGAGAGCAATCATCAAATTCAAGTCGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGcc                                                                                   <  1:128579/135‑1 (MQ=255)                                                     tAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGcc                                                                                   <  4:303526/138‑1 (MQ=255)                                                     tAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGcc                                                                                   <  6:215360/138‑1 (MQ=255)                                                          gaggagATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCtt                                                                             <  5:118075/139‑1 (MQ=255)                                                            ggagATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTcc                                                                           <  4:387644/139‑1 (MQ=255)                                                                      ccGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATg                                                                   <  4:90035/137‑1 (MQ=255)                                                                      ccGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTAGTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATg                                                                   <  6:308268/137‑1 (MQ=255)                                                                          aTGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTTAGTTTTTTTTATACGAACATAAACGAGCTGGGCAATAAAAAAGGGCAGGCCCCGCATACCGCTGATGCTGttt                                                             >  8:118282/1‑139 (MQ=255)                                                                              gagCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGa                                                         <  2:166584/139‑1 (MQ=255)                                                                                gaaaTCATCAAATTCATGTGGGCAATTGAAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGTGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGaa                                                        <  5:230104/136‑1 (MQ=255)                                                                                                aGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTTAGTTTTTTTTGTCCGCACTTAAACGAGCGGGGCAATCAAAAGGGGCCGGCCCCGCTTCCCCCGGGTGCTGTTTCAGAAAGGGCGAACGcccattt                                       >  3:47743/1‑134 (MQ=255)                                                                                                    ggCAATTGCAGCCATAGTATTAGGACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAACGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTaaaa                                   <  7:149700/139‑1 (MQ=255)                                                                                                    ggCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTaaaa                                   <  3:61021/139‑1 (MQ=255)                                                                                                    ggCAATTGCAGCCATAGTATTAGCACTTACCGTCGTGTTGTGGTTGTTTTATGCGAACATAACCGAGCTGGACAAACAAAAAGGGGACGCCCCCCTTCCCCCTGAGGCTGGTTTATCAATGGCAACCGCCAATTTtaa                                    >  6:253291/1‑138 (MQ=255)                                                                                                        aTTGCAGCCATAGTATTAGCACTTACCGGCGATTTGAGTTTGTGTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAg                               <  7:204090/139‑1 (MQ=255)                                                                                                                    gTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACa                   <  5:202738/139‑1 (MQ=255)                                                                                                                    gTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAACGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACa                   <  1:377094/139‑1 (MQ=255)                                                                                                                          gCACTTACCGTCGTTTTTAGTTTTTTTTATCCGAACATAACCGACCGGGCCAAAAAAAAAGGGGAGGCCCACCTTCCCCCGGCAGCAGGGTGAGAAAGGGCCAACCCCATTTTAAAAAAAACGGGGGAAGACaataaat             >  6:333869/1‑137 (MQ=255)                                                                                                                             cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAGGGAGCTGGCCAATCTAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGCGCAAACGCCATTTTAAAACAAGCGGGAGTAGACAATAATTCGa          <  4:172075/139‑1 (MQ=255)                                                                                                                             cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGa          <  7:3619/139‑1 (MQ=255)                                                                                                                              ttACCGTCGTTTTGAGTTTTTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  5:19451/139‑1 (MQ=255)                                                                                                                              ttACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  2:328775/139‑1 (MQ=255)                                                                                                                              ttACCGTCGTTTTGAGTGTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCCGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  4:28415/139‑1 (MQ=255)                                                                                                                              ttACCGTAGTTTTTAGTTTGTTTTCTACGAACTTAACGGAGATGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  8:205908/139‑1 (MQ=255)                                                                                                                               tACCGTCGTTTTGAGTTTGTTTTCTACGAACTTAAGGGAGCTGGCCAATCAAAAGGGGGTGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACATGCGGGAGAAGACTATAATTCGAt         <  4:79529/138‑1 (MQ=255)                                                                                                                               tACCATCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                         >  5:217360/1‑58 (MQ=255)                                                                                                                               tACCATCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                         <  6:217360/58‑1 (MQ=255)                                                                                                                                tccGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGGCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  6:180250/136‑1 (MQ=255)                                                                                                                                cccGTCGTTTTGTGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACTCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  8:113592/136‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  7:118282/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  2:239969/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  6:373571/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  7:85772/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  5:96692/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  5:53127/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  5:333869/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  3:77763/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  1:194433/137‑1 (MQ=255)                                                                                                                                aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  8:269472/137‑1 (MQ=255)                                                                                                                                aCCGGCGTTTTGAGTGTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt         <  8:434641/137‑1 (MQ=255)                                                                                                                                  cgtggtTTTGAGTTTGTTTTCTCCGAACTTAACGGATCTGGACAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTGCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAgt     <  3:203029/139‑1 (MQ=255)                                                                                                                                  cgtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAgt     <  3:167279/139‑1 (MQ=255)                                                                                                                                     cgtGTTGAGTTTGATTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTGTa  <  2:282281/139‑1 (MQ=255)                                                                                                                                      gtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAgtgaa  <  3:388950/138‑3 (MQ=255)                                                                                                                                        |                                                                                                                                        AGGAACACTTACATTCTATCATCGAGATATTCACCATTTTAAAAAGCAAATTTAAAGGAGGAGATTCTGCCGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCCATAGTATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCCGCTGATGCTGTTTCAGAAAGGGCAAACGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTGTA  >  CP006881/572349‑572619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑