Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 711,294 T→C 5.6% K435R (AAA→AGA)  U712_03445 ← Uncharacterized protein yezG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881711,2940TC 5.6% 224.9 / 2.6 71K435R (AAA→AGA) U712_03445Uncharacterized protein yezG
Reads supporting (aligned to +/- strand):  ref base T (36/31);  new base C (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCTTCTTTAAGCGTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATTTGCTTGCTGCTGTTTTGACTTTGGT  >  CP006881/711169‑711421
                                                                                                                             |                                                                                                                               
gTCGTTCTTTAAGCGTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGtttttt                                                                                                                    >  1:436342/1‑139 (MQ=255)
   cttcttTAAGCGTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGa                                                                                                                 <  7:188379/139‑1 (MQ=255)
      cttTAAGCGTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGttttttttga                                                                                                                <  5:146131/137‑4 (MQ=255)
      cttTAAGCGTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATAc                                                                                                              >  3:59778/1‑139 (MQ=255)
         ctggCGTGACCCCGTTGACTGGGTTTTAAGGAACGCCGGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTc                                                                                                           <  2:198827/136‑1 (MQ=255)
          aaGCGTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTc                                                                                                           <  3:3530/138‑1 (MQ=255)
              gTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGc                                                                                                      <  5:140617/139‑1 (MQ=255)
                 aCCCCGTTGCCTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTGAAGTTTGTTTTGTCGTGTTGTTTTGTTCCTTCACTggatg                                                                                                   >  2:215375/1‑135 (MQ=255)
                 aCCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCtg                                                                                                   >  8:57237/1‑139 (MQ=255)
                 aCCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCtg                                                                                                   >  3:308714/1‑139 (MQ=255)
                 aCCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCtg                                                                                                   >  2:36878/1‑139 (MQ=255)
                     cGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGtt                                                                                               <  6:229813/139‑1 (MQ=255)
                       ttGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTgtgtctg                                                                                             >  1:198827/1‑136 (MQ=255)
                          aCTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACtt                                                                                          <  7:363232/139‑1 (MQ=255)
                          aCTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACtt                                                                                          <  7:57237/139‑1 (MQ=255)
                               gTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTtttgt                                                                                     <  4:59778/139‑1 (MQ=255)
                                   tAAGGAACGCCTGCCGGCACTAAATCATGACGGTGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTtttgtttg                                                                                  <  2:403495/138‑1 (MQ=255)
                                     aGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGAt                                                                                <  5:439847/138‑1 (MQ=255)
                                     aGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGAt                                                                                <  7:16252/138‑1 (MQ=255)
                                          cGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGc                                                                                                      >  6:310798/1‑111 (MQ=255)
                                          cGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGc                                                                                                      <  5:310798/111‑1 (MQ=255)
                                          cGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGAt                                                                           >  6:249551/1‑138 (MQ=255)
                                             cTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACtt                                                                                          >  3:88439/1‑120 (MQ=255)
                                             cTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACtt                                                                                          <  4:88439/120‑1 (MQ=255)
                                             cTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAAc                                                                       >  6:26384/1‑139 (MQ=255)
                                             cTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAAc                                                                       >  8:268159/1‑139 (MQ=255)
                                             cTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAAc                                                                       <  1:133596/139‑1 (MQ=255)
                                             cTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAAc                                                                       >  7:362676/1‑139 (MQ=255)
                                             cTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATAATTCAATGACTGTGTTTAGTTTTTGTTTGATGTGCTCAAc                                                                       >  8:5989/1‑139 (MQ=255)
                                                ccGGCCCTAAATCATGACGGGGAGCATACGGCAGAATCTCCGCAAGTGTGAGAGCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGgt                                                                    <  5:240924/139‑1 (MQ=255)
                                                ccGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCTAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGgt                                                                    <  5:50231/139‑1 (MQ=255)
                                                           tCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTgaga                                                         <  2:320301/139‑1 (MQ=255)
                                                           tCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTgaga                                                         <  8:100228/139‑1 (MQ=255)
                                                           tCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTgaga                                                         <  5:292903/139‑1 (MQ=255)
                                                              tGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGAt                                                                                <  7:31933/113‑1 (MQ=255)
                                                              tGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGAt                                                                                >  8:31933/1‑113 (MQ=255)
                                                              tGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTg                                                      >  4:238165/1‑139 (MQ=255)
                                                               gACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGt                                                     >  2:322073/1‑139 (MQ=255)
                                                                   gggATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATg                                                 <  5:4362/139‑1 (MQ=255)
                                                                     gatgatACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACttt                                                                                         <  1:28329/97‑1 (MQ=255)
                                                                     gatgatACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACttt                                                                                         >  2:28329/1‑97 (MQ=255)
                                                                         atACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCtttt                                           >  3:162820/1‑139 (MQ=255)
                                                                               gagaATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCt                                     >  7:392994/1‑139 (MQ=255)
                                                                               gagaATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCt                                     >  6:78478/1‑139 (MQ=255)
                                                                               gagaATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTGTTTGGGCt                                     >  6:246790/1‑139 (MQ=255)
                                                                                   atatCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTg                                 <  2:309823/139‑1 (MQ=255)
                                                                                    tatCCGCATGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGCGCTCAACGGTTTGTTTTGAGACTGTTATGGCTTTTAGGGCTGTGGc                                >  6:245492/1‑139 (MQ=255)
                                                                                    tatCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGc                                >  1:40352/1‑139 (MQ=255)
                                                                                    tatCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGc                                >  6:291554/1‑139 (MQ=255)
                                                                                       ccGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTAAATGCATGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGa                             >  5:147140/1‑139 (MQ=255)
                                                                                         gCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGAt                                                                                >  7:184485/1‑86 (MQ=255)
                                                                                         gCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGAt                                                                                <  8:184485/86‑1 (MQ=255)
                                                                                         gCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGAtt                           >  7:29572/1‑139 (MQ=255)
                                                                                             gtgtGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATttgct                       <  5:78478/139‑1 (MQ=255)
                                                                                                tgAGATCTCCTATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATttgcttgc                    >  5:288408/1‑139 (MQ=255)
                                                                                                tgAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAAGGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATttgcttgc                    >  2:155203/1‑139 (MQ=255)
                                                                                                tgAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTa                                                   <  3:120113/108‑1 (MQ=255)
                                                                                                tgAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTa                                                   >  4:120113/1‑108 (MQ=255)
                                                                                                tgAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATttgcttgc                    >  1:20631/1‑139 (MQ=255)
                                                                                                       tcCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGgtt                                                                   <  3:193697/85‑1 (MQ=255)
                                                                                                       tcCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGgtt                                                                   >  4:193697/1‑85 (MQ=255)
                                                                                                         cGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTCGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATTTGCTTGCTGCTGtt             <  8:362676/137‑1 (MQ=255)
                                                                                                         agatttgatttAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTCTTTACTATTAGTTTGATGTTATCAACGGTTTGTTTTGAGACTCTTATGGCTTTTTGGGCTGTTGCTGATTTGCTTGCTGCTGtttt           >  5:268211/2‑139 (MQ=255)
                                                                                                              tgatttAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATTTGCTTGCTGCTGTTTTGACtt      >  6:218722/1‑139 (MQ=255)
                                                                                                                 tttAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGgt                                                                    <  7:304879/74‑1 (MQ=255)
                                                                                                                 tttAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGgt                                                                    >  8:304879/1‑74 (MQ=255)
                                                                                                                   tAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATTTGCTTGCTGCTGTTTTGACTTTGgt  <  5:249551/138‑1 (MQ=255)
                                                                                                                          gTTCTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTga                                                           <  7:257727/74‑1 (MQ=255)
                                                                                                                          gTTCTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTga                                                           >  8:257727/1‑74 (MQ=255)
                                                                                                                          gTTCTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTga                                                           >  8:264166/1‑74 (MQ=255)
                                                                                                                          gTTCTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTga                                                           <  7:264166/74‑1 (MQ=255)
                                                                                                                             |                                                                                                                               
GTCCTTCTTTAAGCGTGACCCCGTTGACTGCGTTGTAAGGAACGCCTGCCGGCACTAAATCATGACGGGGATGATACGGGAGAATATCCGCAAGTGTGAGATCTCCGATTTGATTTAACTTGGTTTTGACGTGTTTTTTTGATACTTCAATGCCTGTGTTTACTTTTTGTTTGATGTGATCAACGGTTTGTTTTGAGACTGTTATGGCTTTTTGGGCTGTTGCTGATTTGCTTGCTGCTGTTTTGACTTTGGT  >  CP006881/711169‑711421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑