Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,725,082 A→C 100% A29A (GCT→GCG)  U712_19205 ← Uncharacterized protein ywcI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,0820AC100.0% 113.7 / NA 33A29A (GCT→GCG) U712_19205Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/19);  total (14/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAG  >  CP006881/3724993‑3725214
                                                                                         |                                                                                                                                     
ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                                                      <  3:310819/139‑1 (MQ=255)
       ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  1:29804/139‑1 (MQ=255)
       ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  1:314053/139‑1 (MQ=255)
       ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  3:417530/139‑1 (MQ=255)
       ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  7:419929/139‑1 (MQ=255)
       ccATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  3:316044/139‑1 (MQ=255)
          tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCGTTTCTTTTGTTTGTAAGCGGAGCCGAATATACCGCGGCAGCCGCAGTTTTTATGTGCGGCGGCACCCCGGTACACAAAAACACCATCCccac                                                                            >  8:227681/1‑139 (MQ=255)
                                                 cagccgccTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTGATAGAAAAACATcc                                     <  7:227681/137‑1 (MQ=255)
                                                    ccgcGTTTCGTTGGTATGTACGCTGGGCCGAATTGACCGCGGCAGCCGCAGTTTTTCTGTCCGGGGGCACCCCGTTGCTGAAAAAGCCCCTCCCCACCCGGAAAGCTACAAGCATAGGGTGCTTTGAAAAACGTGCCTc                                  >  4:425947/1‑139 (MQ=255)
                                                    ccgcCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATcccac                                  >  1:23880/1‑137 (MQ=255)
                                                    ccgcCTTTCTTTGGTATGTAAGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTc                                  >  1:16656/1‑139 (MQ=255)
                                                     cgccgttCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCCCACCCGGAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTcc                                 >  1:121887/6‑139 (MQ=255)
                                                              ttGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCAAACCCCTAAACATACAAGCAAACGTATCATAGAAAAACATCCATCCGCTGAtttt                        >  7:94858/1‑139 (MQ=255)
                                                                      tACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggc                <  3:189644/139‑1 (MQ=255)
                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTACAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  3:404922/138‑1 (MQ=255)
                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  5:9091/138‑1 (MQ=255)
                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  5:365729/138‑1 (MQ=255)
                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  7:398208/138‑1 (MQ=255)
                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  8:50026/138‑1 (MQ=255)
                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  3:289795/138‑1 (MQ=255)
                                                                        cGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  1:400457/138‑1 (MQ=255)
                                                                         gCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGc             <  6:161143/139‑1 (MQ=255)
                                                                           tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               >  4:134592/1‑135 (MQ=255)
                                                                           tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTggcaggca               <  3:134592/135‑1 (MQ=255)
                                                                           tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGgccgccg           >  1:339178/1‑138 (MQ=255)
                                                                           tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑t          >  2:1625/1‑139 (MQ=255)
                                                                            gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCCCACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAGCC‑tt         >  1:140874/1‑139 (MQ=255)
                                                                            gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTGTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGgccgccgt          >  6:365729/1‑137 (MQ=255)
                                                                              gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               >  3:87859/1‑68 (MQ=255)
                                                                              gCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCca                                                                               <  4:87859/68‑1 (MQ=255)
                                                                                  aaTAGACCGCGGCAGCCGCTGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGCCCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATACCTCCGCTTCGTTTTGGCAGGCAGCC‑tttttag    >  3:27401/1‑135 (MQ=255)
                                                                                  aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCCCACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑tttttag    >  3:222290/1‑135 (MQ=255)
                                                                                    tAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTagcag  <  5:133854/139‑1 (MQ=255)
                                                                                         |                                                                                                                                     
TTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAG  >  CP006881/3724993‑3725214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑