Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,960,740 C→A 10.4% G397G (GGG→GGT)  U712_20420 ← Hypothetical Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,960,7400CA10.4% 306.1 / 2.3 122G397G (GGG→GGT) U712_20420Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base C (61/43);  new base A (14/2);  total (77/45)
Fisher's exact test for biased strand distribution p-value = 2.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GTACGCCGCAATACAGCGCGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTGCGCAAACTTTGTCAG  >  CP006881/3960607‑3960874
                                                                                                                                      |                                                                                                                                      
gcaCGCCGCAATACAGCGCGATACGCTGCTCCGGAACAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGcc                                                                                                                                   <  6:79597/137‑1 (MQ=255)
 tACGCCGCAATACAGCGCGATACGCTGCTCCGGCTCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTGAAATCTTCGATCATCCCGccc                                                                                                                                  >  3:395274/1‑139 (MQ=255)
 tACGCCGCAATACAGCGCGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGccc                                                                                                                                  >  3:349908/1‑139 (MQ=255)
 tACGCCGCAATACAGCGCGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGccc                                                                                                                                  >  3:178957/1‑139 (MQ=255)
   cgccgcAATACAGCGCGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTc                                                                                                                                >  5:260411/1‑139 (MQ=255)
         aaTACAGCGCGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGat                                                                                                                          <  4:52629/139‑1 (MQ=255)
           tACAGCGCGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCATATATATTCCCGCCCTCACGGata                                                                                                                         >  6:244263/1‑138 (MQ=255)
           tACAGCGCGATACGCGGCTGCGGATGAAATTCAAGACTGATGAGCACGACCGTATTTAACGTTAGGGCGATCCGCGGAAGCCCCGGGTTCCGCGGT‑GGTTTTAATTTCAAATCTTGGATCTTTCCGCCCTCCCGGgtat                                                                                                                        >  4:441902/1‑139 (MQ=255)
               gcgcGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTGTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATAttt                                                                                                                    >  5:356614/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGTATCAAATTCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACATGGTACAGAGGT‑GTTTTAAATTTAAAATCTTATATCTTACCGCCCTACCGGATATATTTg                                                                                                                   >  4:79423/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTGTCTTACCGCCCTCACGGATATATTTg                                                                                                                   >  8:179254/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTGCCGCCCTCACGGATATATTTg                                                                                                                   >  7:233400/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCAc                                                                                                                              >  5:218697/1‑128 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATAttt                                                                                                                    >  8:85444/1‑138 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTg                                                                                                                   >  7:301627/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTg                                                                                                                   >  7:323619/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTg                                                                                                                   >  8:100750/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTg                                                                                                                   >  4:199840/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTAAAATATTCTATATTCCCGCCCTCACGGATATAATTg                                                                                                                   >  2:201444/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTAAAAGGCTGTATCTTCCCGCCCCCACCGAGATttttt                                                                                                                   >  5:5035/1‑138 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GGTTTAAATTTCAAATCTTCTATCTTACCGCCCGGACGGGTATATTTg                                                                                                                   >  3:252961/1‑139 (MQ=255)
                cgcgATACGCTGCTCCGGATCAAATGAAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCAc                                                                                                                              <  6:218697/128‑1 (MQ=255)
                   cttccGCTGCTCCGCATCAAATGCATTACTGATCCCCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGt                                                                                                                <  3:23183/135‑1 (MQ=255)
                     tccggtgctCCGTATAAAATCCAAGACTGAAGACCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGt                                                                                                                <  1:201444/132‑1 (MQ=255)
                     tACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAAACTTCTATCTTCCCGCCCTGCCGCATAAATTTGCGtgt                                                                                                              >  1:390811/1‑137 (MQ=255)
                        gctgctCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGaa                                                                                                           <  7:100750/139‑1 (MQ=255)
                          ttctCCGGAAAAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACa                                                                                                         <  5:244263/137‑1 (MQ=255)
                            ctCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGTGGTTTTAAATTT‑AAAAAATATATATTACCGCCCACACGGATATAATTGCTTGTGAACAt                                                                                                        >  3:260280/1‑138 (MQ=255)
                            ctCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCCCGGAGATATTTTCGTGTGAACAt                                                                                                        >  3:58425/1‑138 (MQ=255)
                            ctCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGAAATATTTGCGTCGGAACAt                                                                                                        >  7:102022/1‑138 (MQ=255)
                            ctCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GGATTAAAATTCAAATAATCTATATTCCCGCCCACACGGATATAATTGCGTCTGAACAt                                                                                                        >  3:392071/1‑138 (MQ=255)
                                  aTCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTgg                                                                                                 <  4:89664/139‑1 (MQ=255)
                                      aaTCCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAAACTTCTCAATTCCCGCCCGCCCCGATATTATTGGGTGTGAACAAGAtgtgtgg                                                                                               >  5:40390/1‑134 (MQ=255)
                                          cAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCga                                                                                         <  7:85444/139‑1 (MQ=255)
                                                  gtcaGCACGACCGTATTTACGGTTTGGGCGATCCGCGGAAGCACCGGGTACAGCGGG‑TTTTTAAATTTCAATTCTTCTATCTTCCGGCCCACACGGATATATTTGGTTCTCAACATGTTTTGCTAACGCGTGCTCGTaa                                                                                 <  4:395274/136‑1 (MQ=255)
                                                  atgaGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTaa                                                                                 >  1:80327/1‑139 (MQ=255)
                                                  atgaGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTa                                                                                  >  8:267081/1‑138 (MQ=255)
                                                       cACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCAATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAg                                                                            >  6:305951/1‑139 (MQ=255)
                                                       cACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAAATTAAAAACTTAAATATTACCGCCCTCCCGTCTATTTATTGGTCAGAACATGATTTTGGAATACGAGATCGGaaaaatg                                                                            >  6:258970/1‑134 (MQ=255)
                                                        aCGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTAAAATCTTCTATCTTCCCGCCCTCACGGATATATTTTGGTCTGGACAAGATTTGGGTAAGCGAGATCCTAATACAGc                                                                           >  5:14875/1‑139 (MQ=255)
                                                                 tttAACGGTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTgcg                                                                  <  1:432290/139‑1 (MQ=255)
                                                                    aaCGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTTCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTgcgcgc                                                               >  7:387028/1‑139 (MQ=255)
                                                                    aaCGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTgcgcgc                                                               >  6:169364/1‑139 (MQ=255)
                                                                      cGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTTAATTTCAAATCTTCTATGTTACCGCCCTCCCGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATAGTAATCCAGCCGACCTGCGCGCt                                                              >  7:310644/1‑138 (MQ=255)
                                                                      cGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTc                                                             >  7:235141/1‑139 (MQ=255)
                                                                          aGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GGTTTAAATTTCAAATCTTCTATCTTACCGCCCTCACGGATATATTTGCGTCTGGACATGATTTTGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGc                                                         >  4:137651/1‑139 (MQ=255)
                                                                           ggCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAAATTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGcc                                                        >  5:138397/1‑139 (MQ=255)
                                                                               gATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCTCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGATAGCGAGATCGTATTCGAGCCGACCTGCGCGCTCATGCCCGca                                                    <  4:392071/139‑2 (MQ=255)
                                                                               gATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGcc                                                    <  3:73349/139‑1 (MQ=255)
                                                                               gATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGcc                                                    <  6:260411/139‑1 (MQ=255)
                                                                                aTCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCCTGCCCGcc                                                    >  1:41297/1‑138 (MQ=255)
                                                                                aTCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTACCGCCCTCCCGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGcc                                                    >  8:228516/1‑138 (MQ=255)
                                                                                aTCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTAGATCTTACCGCCCTACCGGATATATTTTCGGCTGAACATGATTTTTGTAAGCGAGCTCGTAATCCAGCCGACCTGCCCGCGCAAGcccccc                                                    >  8:393043/1‑138 (MQ=255)
                                                                                aTCAGCGCAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTACCGCCCTCACGGATATATTTGCGTCTGAACATGATTTTGGACAGCGAGATAGTAAACCAGCCGACCTGCGCTCTCATGCCCGcc                                                    >  8:429476/1‑138 (MQ=255)
                                                                                   aGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCaaa                                                 <  2:80327/138‑1 (MQ=255)
                                                                                   aGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCaaa                                                 <  2:269151/138‑1 (MQ=255)
                                                                                   aGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCaaa                                                 <  4:130707/138‑1 (MQ=255)
                                                                                   aGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCaaa                                                 <  7:393043/138‑1 (MQ=255)
                                                                                   aGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCaaa                                                 <  8:301627/138‑1 (MQ=255)
                                                                                   aGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGGCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCaaa                                                 <  6:371176/138‑1 (MQ=255)
                                                                                       gAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATaa                                            <  4:231797/139‑1 (MQ=255)
                                                                                       gAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATaa                                            <  7:267081/139‑1 (MQ=255)
                                                                                       caaGCAACGGGTACAGCCGT‑GTTTTAAATTTCAAATATTCTATCTTACCTCCCTCAATGATATATTTGCGTCTGAACTTGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGAGCTCATGCCCGCCAAAGATaa                                            <  1:203315/138‑1 (MQ=255)
                                                                                         aGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACa                                          <  6:150315/139‑1 (MQ=255)
                                                                                          gCACCGGGTACAGCGGT‑GGTTTTAATTTCAAATCTTCTATGTTCCCGCCCTCCCGGATATATTTGCGTCTGAACATGATGTGGGAAAGCGAGATCGTAAGCCAGCCGCCCTGCGCGCTCCTGCCCGCCAAAGGTAAccg                                         >  4:282633/1‑137 (MQ=255)
                                                                                          gCACCGGGTACAGCGGT‑GGTTTAAATTTGACATCTACGAACTTACCGCCCCCACCGATATATTTGCCTCTGAACATGATTTGGTAACGCCAGATCGTAATCCAGACCAACTTCGCGCTCAATCCCGCCAAAGATAACa                                          >  8:30032/1‑138 (MQ=255)
                                                                                           cACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGt                                        <  3:123999/139‑1 (MQ=255)
                                                                                           cACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGt                                        <  1:217875/139‑1 (MQ=255)
                                                                                           cACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGt                                        <  4:178957/139‑1 (MQ=255)
                                                                                           cACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGt                                        <  6:356614/139‑1 (MQ=255)
                                                                                             ccGGGTACATCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGTATATATTTGCTTCTTAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTAc                                      <  4:260280/139‑1 (MQ=255)
                                                                                               gggTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTc                                                                                                                                >  6:183046/1‑47 (MQ=255)
                                                                                               gggTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTc                                                                                                                                <  5:183046/47‑1 (MQ=255)
                                                                                               gggTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATaa                                            >  6:406747/1‑131 (MQ=255)
                                                                                               gggTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATaa                                            <  5:406747/131‑1 (MQ=255)
                                                                                                     aGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGt                                        <  6:15559/129‑1 (MQ=255)
                                                                                                     aGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGt                                        >  5:15559/1‑129 (MQ=255)
                                                                                                     aGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCAtatata                              <  3:441902/139‑1 (MQ=255)
                                                                                                     aGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCGGAACATGATTTGGGTAAGCGAGAGCGTAATCCAGCCGCCCTGCGCGCTCATGCCCGCCAAAGCTAACAGTACCAtatata                              >  2:46023/1‑139 (MQ=255)
                                                                                                     aGCGGT‑GTTTTAAATATCACATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTCAACATGATTAGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCGCATGCCCGCCAAAGATAACAGTCCCAtatata                              <  3:264231/139‑1 (MQ=255)
                                                                                                     aGCGGT‑GTTATAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCAtatata                              <  4:252961/139‑1 (MQ=255)
                                                                                                       cGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACg                            >  2:386588/1‑139 (MQ=255)
                                                                                                       cGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCGCATGCCCGCCAAAGATAACAGTACCATATATACg                            >  7:63696/1‑139 (MQ=255)
                                                                                                          t‑gtTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTTCGTCTGAACATTATTTGGGAAAGCGAGATCGTAATCCACCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGtt                         >  3:186558/1‑139 (MQ=255)
                                                                                                          t‑gtTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGCCCTGCGCGCGCATGCCCGCCAAAGATAACAGTACCATATATACGGtt                         >  6:440754/1‑139 (MQ=255)
                                                                                                          t‑gtTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATaa                                            >  7:245636/1‑120 (MQ=255)
                                                                                                          t‑gtTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGtt                         >  3:223673/1‑139 (MQ=255)
                                                                                                          t‑gtTTTAAATTTCAAATCTTCTATCTTACCGCCCGCACGGATATATTTGAGTCTGAACATGATTTGGGAAATCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGtt                         >  3:7459/1‑139 (MQ=255)
                                                                                                          t‑gtTTTAAATTTCAAATATTATATCTTCCCGCCCGCACGGATATATTTTCGTCTGAACATGATTTGGGAAAGCGAGATAGTAATCCAGCCGACCTTCGCGCTCATTCCCGCCAAAGATAAAAGTAACATATATACGGtt                         >  8:232827/1‑139 (MQ=255)
                                                                                                          t‑gtTTTAAATTTAAAATCTTGTATCTTCCCGCCCGCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGtt                         >  6:121596/1‑139 (MQ=255)
                                                                                                             ttttAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGCACTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATAAACGGttt                        >  8:321181/1‑138 (MQ=255)
                                                                                                             ttttAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGttt                        >  6:308739/1‑138 (MQ=255)
                                                                                                              tttAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGttt                        >  1:25946/1‑137 (MQ=255)
                                                                                                                 aaaTTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGc                    <  6:14875/138‑1 (MQ=255)
                                                                                                                     ttCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTgcg               >  1:279461/1‑139 (MQ=255)
                                                                                                                      tCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTgcg               <  6:138397/138‑1 (MQ=255)
                                                                                                                                      cccGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTGCGCAAACTTTGTCAg  <  8:229082/135‑1 (MQ=255)
                                                                                                                                      cccGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTGCGCAAACTTTGTCAg  <  2:25946/135‑1 (MQ=255)
                                                                                                                                      cccGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTGCGCAAACTTTGTCAg  <  5:305951/135‑1 (MQ=255)
                                                                                                                                      cccGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTGCGCAAACTTTGTCAg  <  3:152800/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GTACGCCGCAATACAGCGCGATACGCTGCTCCGGATCAAATGCAAGACTGATGAGCACGACCGTATTTAACGTTAGGCCGATCAGCGGAAGCACCGGGTACAGCGGT‑GTTTTAAATTTCAAATCTTCTATCTTCCCGCCCTCACGGATATATTTGCGTCTGAACATGATTTGGGAAAGCGAGATCGTAATCCAGCCGACCTGCGCGCTCATGCCCGCCAAAGATAACAGTACCATATATACGGTTTCAGCCTGCGCAAACTTTGTCAG  >  CP006881/3960607‑3960874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑