Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 35,960 A→G 35.8% Y39C (TAT→TGT)  U712_00130 → Protein xpaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00688135,9600AG35.8% 73.7 / 65.3 81Y39C (TAT→TGT) U712_00130Protein xpaC
Reads supporting (aligned to +/- strand):  ref base A (3/45);  new base G (2/22);  total (14/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAA  >  CP006881/35824‑36092
                                                                                                                                          |                                                                                                                                    
gACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTAt                                                                                                                                     <  6:415164/138‑1 (MQ=255)
gACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTAt                                                                                                                                     <  3:282615/138‑1 (MQ=255)
    caGGAGTCGATTATGGCAAGCAGAGATTTTTTCACTTTTTTGTGAGGAGTTTAAATAGCAATGCGCAGTTTGTTTTAATTTGAATATTGAGCGTTTTTGGTTTTAACCCGTCGAGGTTTTTTTAT‑TATTGTTTAggggg                                                                                                                                 >  6:101904/1‑138 (MQ=255)
    caGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATTTTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGgct                                                                                                                                <  3:195374/139‑1 (MQ=255)
    caGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGgct                                                                                                                                <  1:166675/139‑1 (MQ=255)
    caGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGgct                                                                                                                                <  2:339860/139‑1 (MQ=255)
    caGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGgct                                                                                                                                <  8:352524/139‑1 (MQ=255)
    caGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGgct                                                                                                                                <  1:331987/139‑1 (MQ=255)
    caGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGgct                                                                                                                                <  1:48410/139‑1 (MQ=255)
          tCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTc                                                                                                                          <  1:221624/139‑1 (MQ=255)
               cagctcATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  3:377270/136‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGag                                                                                                                     <  8:101150/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGag                                                                                                                     <  3:18419/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGag                                                                                                                     <  1:220539/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGag                                                                                                                     <  8:7544/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  5:295570/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  7:440013/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  5:443612/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  1:33355/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  3:98826/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  7:184789/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  4:112958/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  3:14296/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  5:271328/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTGTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGag                                                                                                                     <  7:239469/138‑1 (MQ=255)
                aTCTCATGCAGAGATTTTTTAACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGag                                                                                                                     <  5:253551/138‑1 (MQ=255)
                     aTGCAGAGATTTTTTGACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGcc                                                                                                                <  3:221862/138‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGagca                                                                                                                   <  1:137753/135‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGcc                                                                                                                <  7:2789/138‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagcagc                                                                                                                 <  1:356770/137‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagcag                                                                                                                  <  1:264339/136‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagca                                                                                                                   <  5:277254/135‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGcc                                                                                                                <  6:178419/138‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGcc                                                                                                                <  7:434354/138‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGcc                                                                                                                <  8:218725/138‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGcc                                                                                                                <  1:209612/138‑1 (MQ=255)
                     aTGCAGAGATTTTTTCACTTTTTGGTGTGGAGGTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGagca                                                                                                                   <  6:312000/135‑1 (MQ=255)
                     aTGCAGAGATTTTTTAACTTTTTGGTGTGGAGTTTCACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAACCCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGcc                                                                                                                <  1:1504/138‑1 (MQ=255)
                     aTGCAGAGATTCTTTCACTTTTTGGTGTGGATGATAACTAGCAGTGCGACTTTTGTTTTCCTTTGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGcgca                                                                                                                   <  6:253713/135‑1 (MQ=255)
                      tGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGCCTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATGTATTTTTTTTTGGCTTGCTTCTGGAGccgccgg                                                                                                              >  6:271328/1‑138 (MQ=255)
                      tGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTTTATTTTTTTTTGGGTTTGTTGTGGAGCAGCCGt                                                                                                              >  5:378504/1‑139 (MQ=255)
                      tGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTATATTTTTTTTTGGGTTTGTTGTGGGGGAGCCgg                                                                                                              >  4:14296/1‑138 (MQ=255)
                        cAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTTTATTTTTTTTTGGGTTGGGTGTGGAGCAGCCGtat                                                                                                            >  2:264339/1‑139 (MQ=255)
                         agagATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGtata                                                                                                           <  3:225490/139‑1 (MQ=255)
                           agaTTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTATATTTTTTTTTGGGGTGGTTGTGGAGCCGCCGgatatt                                                                                                         >  8:113539/1‑138 (MQ=255)
                             aTTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTATATTTTTTTTTGGCTTGATTAGTGAGCAGCCGtatttatg                                                                                                       >  4:377270/1‑138 (MQ=255)
                              ttttttCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAg                                                                                                      <  2:6263/139‑1 (MQ=255)
                                 tttCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCt                                                                                                    <  1:355185/138‑1 (MQ=255)
                                 tttCACTTTTTGGTGTGGAGGTTAACGAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCt                                                                                                    <  8:179872/138‑1 (MQ=255)
                                     aCTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCAt                                                                                               <  7:170318/139‑1 (MQ=255)
                                      cTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATc                                                                                              <  1:2261/139‑1 (MQ=255)
                                       tttttGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCt                                                                                             >  5:186064/1‑139 (MQ=255)
                                         tttGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTg                                                                                            <  1:48262/138‑1 (MQ=255)
                                           tgttgtgGTCTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGT‑‑TTTTTTTATATATTTTTTATGGGCTTGCTTCTGTAGCAGCCGTATATATAGCTGGCATCTGGa                                                                                          <  8:96416/135‑1 (MQ=255)
                                                 ggAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAg                                                                                   <  1:199241/139‑1 (MQ=255)
                                                       tAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGt                                                                              <  8:56360/138‑1 (MQ=255)
                                                             gCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTTTCTATTTTTTGTGGGCTTGGTTCTGGAGCCTCCGGATTTTTTGCTGGCATCTGGTATTCAAGACGGGTAtttgt                                                                       >  2:68331/1‑137 (MQ=255)
                                                             gCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTATTTTTTTTATTGGGTTTCTTCTGGAGGAGCCCTATATATATCTGGCATCTGGAATGCAAAACGGTTAattgt                                                                       >  8:213735/1‑137 (MQ=255)
                                                                   cGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTaaaa                                                                  <  3:95332/138‑1 (MQ=255)
                                                                   cGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTATATTGTTTTTTGGGTTGGTTGTGGGGCAGCCGGATTTATATCTGGCATCGGGGATTGAAGACGGGTTTTTATTtaaa                                                                  >  3:331658/1‑138 (MQ=255)
                                                                   cGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTATCTATTTTTTTTTGGGTTGGGTCTGGAGCAGCCGGATTTATATCTGGGATCTGGAATGGAAGACGGGTTTTTATTaaaa                                                                  >  7:35692/1‑138 (MQ=255)
                                                                               tCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCGTGCTTCTTGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGa                                                      <  8:296298/138‑1 (MQ=255)
                                                                               tCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGa                                                      <  8:276317/138‑1 (MQ=255)
                                                                               tCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGa                                                      <  8:309561/138‑1 (MQ=255)
                                                                               tCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCAGCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGa                                                      <  2:363582/138‑1 (MQ=255)
                                                                               tCATTGGAATATTGAGCTTTTTTGGTGTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGa                                                      <  2:179972/138‑1 (MQ=255)
                                                                                cATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGAc                                                     <  8:2370/138‑1 (MQ=255)
                                                                                   tGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑GTTTTTTTTATTTTTTTTTTTGGGTTTGTTCTGGGGCAGCCGGATATATATCTGGCATCTGGAATTCAAGACGGGTATTTGTTAAAAAACATGAATTTACAgg                                                  >  7:177955/1‑138 (MQ=255)
                                                                                    ggAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGa                                                 <  5:38515/138‑1 (MQ=255)
                                                                                     gAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGaa                                                <  6:186064/138‑1 (MQ=255)
                                                                                     gAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGaa                                                <  2:356031/138‑1 (MQ=255)
                                                                                     gAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGaa                                                <  2:348993/138‑1 (MQ=255)
                                                                                     gAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGaa                                                <  7:113539/138‑1 (MQ=255)
                                                                                       atatTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAagag                                             <  5:150499/139‑1 (MQ=255)
                                                                                       atatTGAGATTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAagag                                             <  6:67451/139‑1 (MQ=255)
                                                                                        tatTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAagaga                                            <  6:90418/139‑1 (MQ=255)
                                                                                          ttGATCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTTTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTTGCATCTGGAATGCAAGACGGTTATTTGTTAATAAGCATGAATTGACAGGAagagag                                           <  3:283564/138‑1 (MQ=255)
                                                                                          ttGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTGTGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGt                                          <  7:75068/139‑1 (MQ=255)
                                                                                                      ggTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACAtt                               <  8:421555/138‑1 (MQ=255)
                                                                                                                             ttATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCa          <  6:378504/138‑1 (MQ=255)
                                                                                                                                     tGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGaa  >  2:162939/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                    
GACACAGGAGTCGATTATCTCATGCAGAGATTTTTTCACTTTTTGGTGTGGAGTTTAACTAGCAGTGCGACTTTTGTTTTCATTGGAATATTGAGCTTTTTTGGTTTAAACCAGTCGA‑‑TTTTTTTATCTATTGTTTATGGGCTTGCTTCTGGAGCAGCCGTATATATAGCTGGCATCTGGAATGCAAGACGGTTATTTCTTAAAAAGCATGAATTGACAGGAAGAGAGTATGCTTACATTAAGAAAAACTTAGAGGAAGCAAGGCAGAA  >  CP006881/35824‑36092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑