BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,146	A→G	90.6%	intergenic (–/‑832)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,146	0	A	G	90.6%	37245.5 / inf	12449	intergenic (–/‑832)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base A (584/585); new base G (5667/5544); total (6268/6181)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-04

Only 100 of 12463 total aligned reads displayed.

CGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGG  >  pSIJ663_pHT315_araE_xy/2009‑2284

 gATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGt                                                                                                                                          <  3:187948/139‑1 (MQ=255)

     tCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTg                                                                                                                                      >  3:416160/1‑139 (MQ=255)

     tCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGATTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTTGAGGTTTTg                                                                                                                                      >  2:260126/1‑139 (MQ=255)

          tATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCcac                                                                                                                                  >  8:70625/1‑138 (MQ=255)

          tATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCcac                                                                                                                                  >  6:211944/1‑138 (MQ=255)

          tATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCAGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCcac                                                                                                                                  >  5:14223/1‑138 (MQ=255)

             gtgCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCacaa                                                                                                                                >  4:242453/1‑137 (MQ=255)

                cGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGtt                                                                                                                           >  5:389316/1‑139 (MQ=255)

                cGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGGTTTGCCACAACAGtt                                                                                                                           <  7:201729/139‑1 (MQ=255)

                cGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAGTTTTACATCGTTAGAAGTGCAAGTCATGTTTTATGTAGCTATAATGATTTGTGGAGGTTTTGCCACAACAGtg                                                                                                                           >  2:388255/1‑138 (MQ=255)

                 gATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGt                                                                                                                            <  6:303215/137‑1 (MQ=255)

                               aTAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATc                                                                                                            >  2:321737/1‑139 (MQ=255)

                               aTAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCAGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATc                                                                                                            >  2:419811/1‑139 (MQ=255)

                                   aTACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCtttt                                                                                                        >  6:241699/1‑139 (MQ=255)

                                                    cTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCaaa                                                                                       >  5:176560/1‑139 (MQ=255)

                                                      ttACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAAtt                                                                                     >  1:363756/1‑139 (MQ=255)

                                                           tGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTcc                                                                                <  7:113267/139‑1 (MQ=255)

                                                             cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCt                                                                              >  1:1941/1‑139 (MQ=255)

                                                             cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCt                                                                              <  8:209124/139‑1 (MQ=255)

                                                             cTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCt                                                                              >  5:300659/1‑139 (MQ=255)

                                                                 ataAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCAt                                                                           <  3:323447/138‑1 (MQ=255)

                                                                 ataAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCAt                                                                           <  6:54409/138‑1 (MQ=255)

                                                                  taAATCCGGGAAACATTTTTACATCGGTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtct                                                                         <  2:220245/139‑1 (MQ=255)

                                                                     ctCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  5:53878/138‑1 (MQ=255)

                                                                     aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  4:108783/139‑1 (MQ=255)

                                                                     aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  7:267397/139‑1 (MQ=255)

                                                                     aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  7:299905/139‑1 (MQ=255)

                                                                     aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  1:4271/139‑1 (MQ=255)

                                                                     aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  8:236284/139‑1 (MQ=255)

                                                                     aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  7:84957/139‑1 (MQ=255)

                                                                       ccGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGtct                                                                    >  1:162087/1‑139 (MQ=255)

                                                                        cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACACCAGTGGGTTTATTTACAATCTTTTTTTTATTAGCCGTCACATGTGGCCCTCATCGCGtctc                                                                   >  6:18210/1‑139 (MQ=255)

                                                                          ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtct                                                                         <  8:403644/131‑1 (MQ=255)

                                                                          ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                 >  8:335738/1‑139 (MQ=255)

                                                                          ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCACTCATCTCGTCTCtt                                                                 >  7:391808/1‑139 (MQ=255)

                                                                          ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGAGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                 >  1:96217/1‑139 (MQ=255)

                                                                           gAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCttt                                                                <  4:277346/139‑1 (MQ=255)

                                                                            aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTta                                                               <  8:177529/139‑1 (MQ=255)

                                                                                       cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                    <  1:576/139‑1 (MQ=255)

                                                                                       cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                    <  1:128437/139‑1 (MQ=255)

                                                                                       cATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                    <  2:55252/139‑1 (MQ=255)

                                                                                         tCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACTATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTATATATCtttatttta                                                  <  5:359799/139‑1 (MQ=255)

                                                                                          cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCAGAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCttttttttat                                                 >  6:270459/1‑139 (MQ=255)

                                                                                          cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattttat                                                 >  6:83284/1‑139 (MQ=255)

                                                                                           gTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATc                                                <  1:398817/139‑1 (MQ=255)

                                                                                            ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  1:329628/116‑1 (MQ=255)

                                                                                            ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAGTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCa                                               <  6:424626/139‑1 (MQ=255)

                                                                                              aGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCAt                                              <  7:331583/138‑1 (MQ=255)

                                                                                               gAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATaa                                            <  2:155831/139‑1 (MQ=255)

                                                                                               gAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATaa                                            <  4:308811/139‑1 (MQ=255)

                                                                                                  gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                         <  6:144252/139‑1 (MQ=255)

                                                                                                  gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                         <  3:262093/139‑1 (MQ=255)

                                                                                                  gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                         <  7:234828/139‑1 (MQ=255)

                                                                                                     caagtcaagtTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  5:239284/107‑1 (MQ=255)

                                                                                                     caagtcaagtTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                       >  2:290553/1‑138 (MQ=255)

                                                                                                     caagtcaagtTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                       >  7:143029/1‑138 (MQ=255)

                                                                                                     caagtcaagtTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAg                                       >  4:20499/1‑138 (MQ=255)

                                                                                                     caagtcaagtTATATGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGt                                      >  6:363998/1‑139 (MQ=255)

                                                                                                               tataTGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                            >  7:419218/1‑139 (MQ=255)

                                                                                                                atatGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                            <  4:168394/138‑1 (MQ=255)

                                                                                                                atatGTATCTATAATGATTTGAGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCg                            <  6:393674/138‑1 (MQ=255)

                                                                                                                   tGTATCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcg                         <  8:142595/138‑1 (MQ=255)

                                                                                                                   tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCATCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACAGTcgc                        >  5:42420/1‑139 (MQ=255)

                                                                                                                       tCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTta                                                               <  8:306476/96‑1 (MQ=255)

                                                                                                                       tCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttta                                                  >  1:29781/1‑109 (MQ=255)

                                                                                                                       tCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                    >  3:228220/1‑139 (MQ=255)

                                                                                                                       tCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                    >  2:352396/1‑139 (MQ=255)

                                                                                                                       tCTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                    >  6:335273/1‑139 (MQ=255)

                                                                                                                       tCTATAATGATTTGAGGATGTTTTGCCACAACAGGTGGTTTATTGACAATGTTTTGTTTATTAGCCGTCAAATGTCTCCCTCAGCTTTGGTGTTTTTATCTTTATTTTATCATAAAGTGTTATTTGAACAGTGGCgggg                    >  2:124898/1‑139 (MQ=255)

                                                                                                                        cTATAATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCggg                   >  5:111638/1‑139 (MQ=255)

                                                                                                                        cTATAATGATTTGTGGAGGTTATGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                    >  8:275150/1‑138 (MQ=255)

                                                                                                                          ataATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                 <  1:232834/139‑1 (MQ=255)

                                                                                                                           tattGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGTAGTATTTGAACCGTCGCGCGGGAct                >  5:84596/4‑138 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATGTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  <  5:331362/137‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                       >  3:355846/1‑132 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  <  3:47581/137‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  <  8:113880/137‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  <  5:271702/137‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  <  4:379710/137‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  <  7:359897/137‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                 <  1:269317/138‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                 <  8:435695/138‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                 <  2:88576/138‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                 <  3:152237/138‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                 <  8:365783/138‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                 <  6:116815/138‑1 (MQ=255)

                                                                                                                           taATGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTATATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  <  4:411455/137‑1 (MQ=255)

                                                                                                                              tGATTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGt             <  3:296003/139‑1 (MQ=255)

                                                                                                                                aTTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt           >  3:78424/1‑139 (MQ=255)

                                                                                                                                aTTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt           <  4:13557/139‑1 (MQ=255)

                                                                                                                                aTTTGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt            >  7:53407/1‑138 (MQ=255)

                                                                                                                                aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt           >  2:190992/1‑139 (MQ=255)

                                                                                                                                 tttGTGGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTGTCTTTATATCTTTTTTTTTTAATATAGGATTATTTGAACCGTCGCGCCTGACAGGTTTa          >  4:3459/1‑139 (MQ=255)

                                                                                                                                   tgtgGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                      <  4:444612/77‑1 (MQ=255)

                                                                                                                                   tgtgGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                  >  3:385407/1‑129 (MQ=255)

                                                                                                                                     tgGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTTTTTGAACCGTCGCGCGGGACAGGTTTATGa       >  7:173438/1‑138 (MQ=255)

                                                                                                                                     tgGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACa                >  4:75333/1‑129 (MQ=255)

                                                                                                                                     tgGAGGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa       <  5:210658/138‑1 (MQ=255)

                                                                                                                                     tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa       >  3:121615/1‑138 (MQ=255)

                                                                                                                                     tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt      >  1:100044/1‑139 (MQ=255)

CGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGG  >  pSIJ663_pHT315_araE_xy/2009‑2284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`