BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,649	A→C	8.0%	Y260S (TAC→TCC) ‡	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,649	0	A	C	8.0%	11774.6 / 216.0	4640	Y260S (TAC→TCC) ‡	ampR
Reads supporting (aligned to +/- strand): ref base A (3033/1131); new base C (15/457); total (3052/1588)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4772 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTA  >  pSIJ663_pHT315_araE_xy/4511‑4787

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                         >  8:183076/1‑139 (MQ=255)

      gACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACacgac                                                                                                                                      >  7:148513/1‑139 (MQ=255)

         cACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                   >  5:223558/1‑139 (MQ=255)

         cACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                   >  7:62012/1‑139 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                   >  1:252313/1‑137 (MQ=255)

             tCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGt                                                                                                                               >  3:299102/1‑139 (MQ=255)

                 cgcgcgGCGCTTCCGGCTGGGTGGTTTTTTTGTGAAAAATCTGGTGCGGGTCAGCGGGGGGCGCGGGGTATCTTTCCCGCACGGGGGCCA‑TTTTTAAAGCCCACCCTTATCTTATTTATCTCCACGACGGGGAGTCAgg                                                                                                                           <  6:356849/139‑1 (MQ=255)

                 cgcgcgGCCCTTCCGGGGGGGTGTTTTATTGCTTAAAAACGTGCCCCCGGTGCGGGGGGGCCTCGCGTTATCATTGCCGCAGTGGGGCCT‑TTTGGTAAGCCCTCCCGTTTCGTATTAATCAACACGACGGGGAGTCAgg                                                                                                                           <  4:417038/139‑1 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                           >  2:252681/1‑139 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                           >  3:194416/1‑139 (MQ=255)

                  gcgcGGCCCTTCCGGCTGGCTGGTTTATTGCTTATAAAGCTGGAGCCGGTGAGCGGGGGTCGCGCGTTATCATTGCAGCACGGGGCCCA‑GATGGTAACCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGc                                                                                                                          <  7:8489/136‑1 (MQ=255)

                  gccgggCCCTGCGGGCGGGCGGGTTTATGGCTTATAACTCGGGCGCCGGGGCGGGGGGGGCGCGGGGTAACATTCCCCCAGGGGGCCCA‑TATGTAAAGCCCGCCCGTATCTTATTAAACACCACGACGGGGAGTCAGGc                                                                                                                          <  4:837/135‑1 (MQ=255)

                   cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGc                                                                                                                          >  1:75812/1‑138 (MQ=255)

                   cTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCAGTATCGTAGTTATCTACACGACGGGGAGTCAGGc                                                                                                                          >  5:322712/1‑138 (MQ=255)

                      ggCCCTTCCGGGTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGCCGGGGAGTCGGGCacc                                                                                                                       >  8:246070/1‑136 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCTGTAGCATTGCAGCACTGGGGCCA‑GATGGTAAGCCATCCCTAATCGTAGTTATATACACGACGGGGAGTCAGGCAAc                                                                                                                       >  5:293408/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCTTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  2:389699/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  7:179227/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  3:73846/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  5:50887/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GAGGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                       >  5:90429/1‑138 (MQ=255)

                          cTTCCGGCTGGCTGTTTTATTGCTTATAAATCTGGCGCGGGTGAGGGTGGGGCGCGGTTTATCATTCCAGCACTGGGGCCA‑GATGGTACGCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATgg                                                                                                                  <  2:283357/139‑1 (MQ=255)

                            tCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAt                                                                                                                >  7:267262/1‑139 (MQ=255)

                                gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACCCGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                                            >  1:404956/1‑139 (MQ=255)

                                gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                                            >  6:315465/1‑139 (MQ=255)

                                gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                                            >  3:129020/1‑139 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                        >  1:280673/1‑139 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                           >  6:195794/1‑138 (MQ=255)

                                                     aaaTCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                           >  4:288498/1‑135 (MQ=255)

                                                              gCCGGTGCGGGTGGGGCTCGGGGTATCTTTCCCGCCCGGGGGCCT‑GATGGTAAGCCCGCCCGTCTCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCt                                                                              <  7:365805/139‑1 (MQ=255)

                                                                gggTGAGCGGGGGTCGCGTGGTATCTTTGCAGCCGTGGGCCAT‑TATGGAAAGCCCCCCCGTATCTTTGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  4:320291/138‑1 (MQ=255)

                                                                cGGTGCGGGTGGGCCGCGCGTTATCATTCCACCCCGGGGGCCA‑GTTGGAAAGCCCTCCCGTTTCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                                            <  1:308362/139‑1 (MQ=255)

                                                                           ggTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGACCGAAATAGCCAGATCGCGGAGATAGGTGCCTCACGGATTAAGCa                                                                 >  4:478/1‑139 (MQ=255)

                                                                           ggTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCa                                                                 >  4:382700/1‑139 (MQ=255)

                                                                            gTCTCGGGTTATGATGGCAGCTCTGGGGCAA‑GATGTTACCCCCTCCCGTTTCGTAGTTCTCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                                                <  7:316189/139‑1 (MQ=255)

                                                                            gTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                                                >  1:335730/1‑139 (MQ=255)

                                                                               tcGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACTAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTgg                                                             >  3:165106/1‑139 (MQ=255)

                                                                                cgcgGATTCATTGCAGCCCTGGGGCCA‑GTGGGAAACCCCGCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                            <  8:401295/139‑1 (MQ=255)

                                                                                         tttccagccCGGGGGCCA‑GATGGAAAGCCCCCCGTTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAg                                                   <  2:17892/135‑1 (MQ=255)

                                                                                         aTTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAg                                                   >  6:418964/1‑139 (MQ=255)

                                                                                           tGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                 >  7:122628/1‑139 (MQ=255)

                                                                                                 ccgggggCCT‑GTTGGTACGCGCGCCCGTTGCGTTGTTACAAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTTATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  5:112257/136‑1 (MQ=255)

                                                                                                 ccTGGGGCCA‑GCTGGCCACCCCCCCCGTCGCGTCGTAACCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGACAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  4:352187/138‑1 (MQ=255)

                                                                                                 agtGGGGCCA‑TTTGGTAAGCCCTCCCGTTTCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  2:232046/137‑1 (MQ=255)

                                                                                                 aCTGGGCCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  2:139770/139‑1 (MQ=255)

                                                                                                 aCTGGGCCAA‑GATGGAACCCCCCCCCGTATCGTAGTAATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                           <  1:141962/139‑1 (MQ=255)

                                                                                                   tGGGGCCA‑GATGGAAACCCCTCCCGTTTCGTAGGTAGCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  7:343216/139‑1 (MQ=255)

                                                                                                   tGGGCCCA‑GAGGGTACGCCCGCCCGTAGCGTAGTCATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  4:192000/139‑1 (MQ=255)

                                                                                                   gggggCCA‑GATGGTACGCCCCCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  5:364062/138‑1 (MQ=255)

                                                                                                   ggggcacA‑GATGGTAAGCCCTCCGGTAACGTCGTTTTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  8:150624/133‑1 (MQ=255)

                                                                                                   ggggcCCA‑GTTGGTAAGCCCTCCCGTATCGTAGTAAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  4:130795/138‑1 (MQ=255)

                                                                                                    ggggCCATGTTGTTACGCCCCCCCTTATCGTTTTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  8:365721/139‑1 (MQ=255)

                                                                                                     gggCCT‑GTTGGAAAGCCCACCCGTAGCGTAGTTAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  6:120139/137‑1 (MQ=255)

                                                                                                     gggCCC‑TATGGTACCCCCCCCCGCAGCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  5:196532/137‑1 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGATATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACGAAGTTTACt                                       >  2:69790/1‑139 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGCTCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                       >  5:25759/1‑139 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  6:154658/137‑1 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  7:213936/137‑1 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                       >  1:368045/1‑139 (MQ=255)

                                                                                                     gggCCA‑GATGGTAAGCCCCCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  6:387539/137‑1 (MQ=255)

                                                                                                       gCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCa                                     >  2:311345/1‑139 (MQ=255)

                                                                                                         caccttGGTAAGCCCCCCCGTATGTTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAGAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCat                                    <  8:218986/134‑1 (MQ=255)

                                                                                                          a‑gagGGTAACCCCTCCCGTCTCGTAGTTAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  5:144228/139‑1 (MQ=255)

                                                                                                          a‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCGGACCAAGTTTACTCata                                   >  1:48554/1‑138 (MQ=255)

                                                                                                          a‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGGCTGGCAACTATGGAGGAACGAAATAGCCAGATCGGTGAGCTAGGTGCCTCAGTGTTTATGGATGGGGACCTGTCTGACCAAGGTTACTCatat                                  >  7:243136/1‑139 (MQ=255)

                                                                                                          a‑GATGGAAAGCCCGCCCGTATCGTCGATAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  8:305533/139‑1 (MQ=255)

                                                                                                          a‑GATGGAAAGCCCCCCCTTATCGTAGTAATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                  <  3:262942/139‑1 (MQ=255)

                                                                                                            gagggaaaGCCCACCCTTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                 <  8:438958/133‑1 (MQ=255)

                                                                                                             aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                 >  1:10353/1‑138 (MQ=255)

                                                                                                                gTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt                             >  3:392911/1‑139 (MQ=255)

                                                                                                                 tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                            >  7:427423/1‑139 (MQ=255)

                                                                                                                 tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt                            >  4:159932/1‑139 (MQ=255)

                                                                                                                       cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  6:48587/120‑1 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGCGTCAGGCAACTATGGATGAACGAAATAGCCAGCTCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgagtga                    >  8:67739/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      >  6:173359/1‑105 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc                                      <  8:282250/121‑1 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                    >  5:393735/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                    >  1:215927/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                    >  4:21204/1‑139 (MQ=255)

                                                                                                                         cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattct                    >  3:341191/1‑137 (MQ=255)

                                                                                                                               tctgaGTTATCTACACGACGGGGAGTCAGTCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGGGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa               <  2:34388/134‑1 (MQ=255)

                                                                                                                               tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCTTTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa               >  4:239083/1‑138 (MQ=255)

                                                                                                                               tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                                >  8:116560/1‑105 (MQ=255)

                                                                                                                               tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATTTATACTTTAGATTGATTTaa               >  6:224885/1‑138 (MQ=255)

                                                                                                                               tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa               >  1:178369/1‑138 (MQ=255)

                                                                                                                               tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa               >  3:421280/1‑138 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                      <  2:106976/96‑1 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  7:290818/109‑1 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                         <  7:391865/109‑1 (MQ=255)

                                                                                                                                  tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt           >  2:99205/1‑139 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                                >  3:221391/1‑100 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt          >  8:335844/1‑138 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt          >  5:425627/1‑138 (MQ=255)

                                                                                                                                    gTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACtt          >  3:4656/1‑138 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                            <  3:371065/85‑1 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt      >  6:269141/1‑139 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt      >  5:168896/1‑139 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt      >  6:89832/1‑139 (MQ=255)

                                                                                                                                       aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt      >  3:99306/1‑139 (MQ=255)

                                                                                                                                         cTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                                            >  2:436020/1‑83 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA‑GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTA  >  pSIJ663_pHT315_araE_xy/4511‑4787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`