BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,049	C→T	100%	intergenic (+318/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,049	0	C	T	100.0%	25360.8 / ‑6.6	7185	intergenic (+318/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (2321/4822); minor base G (15/15); total (2346/4839)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 7379 total aligned reads displayed.

TTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGA  >  pSIJ663_pHT315_araE_xy/4914‑5184

 tttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCtt                                                                                                                                      <  2:134726/138‑1 (MQ=255)

  ttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                     <  2:242815/138‑1 (MQ=255)

     tGCGTGTAATCTGCTCCTTCCAAAAAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgt                                                                                                                                 <  2:201395/139‑1 (MQ=255)

      gcgcGTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                >  7:194353/1‑139 (MQ=255)

       cgtgTAATCTGGTGCTTGCAAACAAAAAA‑‑CCCACCTCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  3:159031/135‑1 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTTCCGGATCAAGAGCTACCAACTCGTTTTCCGAAGGTTACTGGCTTCAGCAGAGCGCAGATACCAAAAACTGTTCTTCTAttgt                                                                                                                               >  1:220156/1‑139 (MQ=255)

       cgcgTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtg                                                                                                                                <  1:100496/138‑1 (MQ=255)

          gTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGc                                                                                                                            <  7:419859/139‑1 (MQ=255)

           tAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                           <  4:380136/139‑1 (MQ=255)

           tAACCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATTAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                           <  6:205569/139‑1 (MQ=255)

                tgctgcTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATGAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGt                                                                                                                      <  5:257137/139‑1 (MQ=255)

                 gctgctTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTGTGTTTGCCGGAGCCAGCGCTACCCACGCGTTTTCCGAAGGGAACTGGCTTCCGCCGCGCGCAGATACCAAATACTGTTCTGGTATTGTAGCCGTGGtt                                                                                                                     >  1:70779/1‑139 (MQ=255)

                   tgctTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTACGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAg                                                                                                                   >  6:365167/1‑139 (MQ=255)

                    gctTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCACCTCTTTTTCCGCAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAgg                                                                                                                  >  8:397164/1‑139 (MQ=255)

                        gCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              >  5:169269/1‑139 (MQ=255)

                         caaacaaaAAC‑‑CCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  3:369577/139‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  1:45906/139‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  1:310160/139‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  2:401698/139‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  4:445008/139‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  5:139028/138‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  3:104183/138‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  5:108945/138‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  3:187034/138‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                              <  7:166105/138‑1 (MQ=255)

                         caaacaaaAAA‑‑ACCACCGCTACCAGCGGCGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  6:173615/139‑1 (MQ=255)

                           aacaaaAAACCACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacc                                                                                                             <  2:303415/139‑1 (MQ=255)

                              aaaaaa‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc                                                                                                        <  6:109340/139‑1 (MQ=255)

                               aaaaa‑‑aCCACCGCTACCAGCGGTGGTTTTTTTGCCGGATCAAGAGCTACCAACTCGTTTTCCCAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACtga                                                                                                        >  2:438543/1‑136 (MQ=255)

                               aaaaa‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc                                                                                                        >  4:215392/1‑138 (MQ=255)

                                aaaa‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa                                                                                                      <  7:295381/139‑1 (MQ=255)

                                 aaa‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                     <  3:340040/139‑1 (MQ=255)

                                   a‑‑aCGACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCTACTCTTTTTCCGAAGGTAACTGGCTTGAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCTCCACTTCAAGaa                                                                                                   <  8:212766/139‑1 (MQ=255)

                                   a‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGTTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  5:386318/139‑1 (MQ=255)

                                   a‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTGTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  1:406339/139‑1 (MQ=255)

                                   a‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  8:431271/139‑1 (MQ=255)

                                   a‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  4:74368/139‑1 (MQ=255)

                                   a‑‑aCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  8:257342/139‑1 (MQ=255)

                                     ccccaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  6:98804/137‑1 (MQ=255)

                                     acccaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  7:385575/137‑1 (MQ=255)

                                       ccaccGCTACCAGCGGTGGTTTTTTTGCCGGATCAAGAGATACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCATATACCAAATACTGTTCTTCTAGTGTATCCGTAGTTAGGCCACCACTTAAAGAAct                                                                                                 <  8:113342/139‑1 (MQ=255)

                                          ccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCCCCACTTCAAGAACTCTg                                                                                              >  1:374949/1‑139 (MQ=255)

                                                cccgCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  5:47474/136‑1 (MQ=255)

                                                ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  3:3986/139‑1 (MQ=255)

                                                ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  4:340385/139‑1 (MQ=255)

                                                ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        <  6:268260/139‑1 (MQ=255)

                                                 ccgCGGTGGTTTGTTTGCCGGATCCAGAGCTACCAACTCTTTTTCCGAATGTAACTGGCTTCAGCAGAGCGCCGATACCAAATACTGTTCTGCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                       <  1:249467/137‑1 (MQ=255)

                                                 cAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                       <  5:196650/139‑1 (MQ=255)

                                                   gCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGc                                                                                     <  4:312355/139‑1 (MQ=255)

                                                   gCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGc                                                                                     <  2:333083/139‑1 (MQ=255)

                                                    cGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAACGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTGTTCTAGTGTAGCCGTAGTTTGGCCACCCCTTCAACAACTCTGTAGCACCGc                                                                                     >  1:158367/1‑138 (MQ=255)

                                                        ggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   >  8:360801/1‑120 (MQ=255)

                                                            tgtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                        >  3:24466/1‑127 (MQ=255)

                                                             gtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                            <  6:331155/138‑1 (MQ=255)

                                                                tgCCTGATCAAGAGCTACCATCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGACCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGTCCACCACTTCAAGAACTCTGTAGCTCCGCCTACATACCTCg                                                                         <  7:130608/138‑1 (MQ=255)

                                                                tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                         <  3:93342/138‑1 (MQ=255)

                                                                 gCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                        <  2:276351/138‑1 (MQ=255)

                                                                 gCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                        <  1:279496/138‑1 (MQ=255)

                                                                  ccGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                      <  1:127455/139‑1 (MQ=255)

                                                                  ccGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                      <  3:209706/139‑1 (MQ=255)

                                                                      aTCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTGAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCt                                                                  <  5:79483/139‑1 (MQ=255)

                                                                         aaGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGTACCGCCTACATACCTCGCTCTGCTAAt                                                               <  4:414537/139‑1 (MQ=255)

                                                                         aaGACCTACCAACTCTTTATCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAAt                                                               <  6:433652/139‑1 (MQ=255)

                                                                                aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCg                                                                                      <  6:296121/109‑1 (MQ=255)

                                                                                aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTa                                                        >  3:5750/1‑139 (MQ=255)

                                                                                aCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAAAACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCACACCAAATCCTGTTa                                                        >  3:131598/1‑139 (MQ=255)

                                                                                      tctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctc                                                                      <  5:28343/119‑1 (MQ=255)

                                                                                          tttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgct                                               <  6:401957/138‑1 (MQ=255)

                                                                                           ttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgctg                                              <  3:432819/138‑1 (MQ=255)

                                                                                                aaGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCtt                                                                                                                                      <  6:62252/45‑1 (MQ=255)

                                                                                                aaGGTAACTGGCTTCAGCAGAGCGCAGATACCAAACACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                         <  8:179651/106‑1 (MQ=255)

                                                                                                  ggTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAg                                       <  1:99863/138‑1 (MQ=255)

                                                                                                    tAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   >  8:292232/1‑76 (MQ=255)

                                                                                                      aCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCg                                  >  1:188004/1‑139 (MQ=255)

                                                                                                       cTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGa                                 <  7:81316/139‑1 (MQ=255)

                                                                                                          gCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAt                                <  5:320577/137‑1 (MQ=255)

                                                                                                             tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGc                                                                   <  1:345800/99‑1 (MQ=255)

                                                                                                             tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAt                                >  7:353723/1‑134 (MQ=255)

                                                                                                                gcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgtg                        <  4:250224/139‑1 (MQ=255)

                                                                                                                    agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   <  2:368003/60‑1 (MQ=255)

                                                                                                                     gcgcAGATACCAATTACTCTTCTTCTTGTGTAGCCGTAGTTAGGCCCCCACTTCAAGCACTCTGTAGCACCGCCTCCTGACCTCGCTCGGCTACTACTGTTACGAGAGGCTGCTGCCACTCTCGATGAGTCGTGTCTTa                   >  7:323372/1‑139 (MQ=255)

                                                                                                                     gcgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTACTCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTa                   >  7:22639/1‑139 (MQ=255)

                                                                                                                         aGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   >  2:7265/1‑55 (MQ=255)

                                                                                                                           aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCCCCACTTCAAGACCTCTGTCGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTCCCAGTGGCTGATGCCTGTGGCGATAATGCGTGGATGACCGGGt             >  2:167234/1‑139 (MQ=255)

                                                                                                                           aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   >  5:419556/1‑53 (MQ=255)

                                                                                                                           aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTGTTACCgggg             >  7:52502/1‑138 (MQ=255)

                                                                                                                           aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGt             <  4:153027/139‑1 (MQ=255)

                                                                                                                              ccAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                         >  8:147420/1‑76 (MQ=255)

                                                                                                                              ccAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTgg          >  6:145673/1‑139 (MQ=255)

                                                                                                                              ccAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTg           <  8:324864/138‑1 (MQ=255)

                                                                                                                                aattACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAGACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGAc        >  5:224574/4‑139 (MQ=255)

                                                                                                                                aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGaa                                                                                                   >  4:127997/1‑48 (MQ=255)

                                                                                                                                aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAg                                       <  3:278377/108‑1 (MQ=255)

                                                                                                                                aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAg                                       >  4:278377/1‑108 (MQ=255)

                                                                                                                                aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGa         >  8:709/1‑138 (MQ=255)

                                                                                                                                 aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCGGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACt       <  7:261231/139‑1 (MQ=255)

                                                                                                                                 aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACt       <  6:233331/139‑1 (MQ=255)

                                                                                                                                      tGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                       <  3:313197/54‑1 (MQ=255)

                                                                                                                                      tGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGa  >  4:184923/1‑139 (MQ=255)

                                                                                                                                      tGTTCTTCTAGTGTAGCCGTAGTTAGGACACCACTTCAAGAACTCTGTAGCAcc                                                                                       >  5:354723/1‑54 (MQ=255)

TTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA‑‑ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGA  >  pSIJ663_pHT315_araE_xy/4914‑5184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`