BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,467	T→G	5.8%	intergenic (+736/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,467	0	T	G	5.8%	16712.1 / 182.9	7711	intergenic (+736/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (3382/3713); new base G (239/356); total (3631/4080)
Fisher's exact test for biased strand distribution p-value = 4.49e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7767 total aligned reads displayed.

GCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACC  >  pSIJ663_pHT315_araE_xy/5329‑5595

gCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                  <  7:427648/139‑1 (MQ=255)

   gACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAg                                                                                                                               >  5:408886/1‑139 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGTGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                              <  8:437071/139‑1 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                              <  4:306472/139‑1 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                              >  5:114506/1‑139 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                              <  1:234428/139‑1 (MQ=255)

    aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                              <  1:293515/139‑1 (MQ=255)

          aTCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGACACGCCTGGTATCGTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGGTGCTCGTCAGGGGGGCg                                                                                                                        >  2:21632/1‑139 (MQ=255)

                tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGcc                                                                                                                   >  5:442693/1‑138 (MQ=255)

                  aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                >  8:240850/1‑139 (MQ=255)

                          ggTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAAc                                                                                                        >  8:404435/1‑139 (MQ=255)

                              ggAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGATCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCa                                                                                                    >  6:337890/1‑139 (MQ=255)

                                    ggAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCAGACTTGAGCGTCGATTTTTTTTATTCAGGTGACGGGGGCGGCCCCGATTGGAAAACACCAGccaac                                                                                              >  1:398955/1‑135 (MQ=255)

                                     gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTCTCGTCAGGGGGGCGGAGCCTATGGAAAAACCCCCGCAAcgc                                                                                             >  6:305357/1‑139 (MQ=255)

                                       gagCGCACGAGGGAGCTTCCAGGGGCAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                           <  7:118688/139‑1 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACTCCTGGTATCTTTAAAGTCATGTCTGGTTTCGCCACCTCTGACATGAGCGTCGATTTTTGTGAAGATCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                        >  8:71107/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGGTGCGCGTCAGGGGGGGGGCGCCTATGGAAAAACGCCAGCAACGCgggcg                                                                                        >  1:161779/1‑136 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTCACGTCAGGGGGGCGGAGCCTATTGAAAAACCCCACCAACgccgccg                                                                                        >  2:85263/1‑138 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGaaa                                                                                                           <  3:396242/120‑1 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCCACGCGGCCt                                                                                        >  7:142690/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                        >  4:80206/1‑139 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCGATGGAAAAACGCCAGCAACGCGGccg                                                                                        >  5:145473/1‑138 (MQ=255)

                                          cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTAGAGCGTCGATGTTTTTGATTCTAATCAGCGGGGCGGATCCTATCGAAAAAACCCAGCAACCCGGcca                                                                                        >  6:139737/1‑138 (MQ=255)

                                             aCGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCGATGGAAAAACGCCAGCAACGCGGCCtgtt                                                                                     >  6:90895/1‑139 (MQ=255)

                                                aGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                  <  7:54022/139‑1 (MQ=255)

                                                 gggAGCTTCCCGGGGGAAACGCCTGGTATCTTTATAGTCGTGTCGGGTTGCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACg                                                                                 <  5:263190/139‑1 (MQ=255)

                                                    aGCTTCCAGGGGTAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGCGCGTCGATTTTTTTTATTCTCGTGAGGGGGGCCGGGCCGAAGGAAAAAACCCACAAACGAGGCCGTTTTAggggt                                                                              >  2:103201/1‑137 (MQ=255)

                                                    aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                              >  3:113105/1‑91 (MQ=255)

                                                    aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGtt                                                                              >  8:142370/1‑139 (MQ=255)

                                                      ctgccAGGGGGAAACGCCGGGTATGTTTATAGTCCTGGCGGGTTGCGCCACCTCTGACTGGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                            <  2:407889/136‑1 (MQ=255)

                                                      cTTCCTGGGGGAAACGCGTGGTATCTTTATAGTCCTGGCGGGGTGCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGGGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                            <  6:258200/139‑1 (MQ=255)

                                                      cTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGCCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGG‑GGAGCCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTCct                                                                           >  3:146543/1‑139 (MQ=255)

                                                       ttCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                             >  4:225997/1‑121 (MQ=255)

                                                           aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                       >  1:72847/1‑139 (MQ=255)

                                                           aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                       <  7:80546/139‑1 (MQ=255)

                                                                 aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgc                                                                 <  7:237086/139‑1 (MQ=255)

                                                                  aaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgct                                                                >  3:173036/1‑139 (MQ=255)

                                                                      ccTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCCGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcc                                                            <  5:300501/139‑1 (MQ=255)

                                                                       cTGGTTTCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTGGCGCGTCGATTTTGGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcct                                                           <  2:295220/139‑1 (MQ=255)

                                                                        tGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                          >  7:270094/1‑139 (MQ=255)

                                                                          ttATCTTTATCGTGCTGTCGGGTCGCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                          <  4:337575/136‑1 (MQ=255)

                                                                          gTATCTTTATAGTCCTGTCGGTTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAATCACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                          <  1:13143/137‑1 (MQ=255)

                                                                          gTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                          <  6:220084/137‑1 (MQ=255)

                                                                          gTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                          <  5:387562/137‑1 (MQ=255)

                                                                           tATCTTTATCGGCCTGGCGGGTGTCGCCACCTCTGACTTGCGCGGCGATTTTTGTGAGGCTCGGCCGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                       <  7:363410/139‑1 (MQ=255)

                                                                              cTTTATTGTCCTGTCGGGTTTCGCCACCTCTGACTTGCGCGTGTATTTTTGTGATGGTCGTGTGGGGGGCGGAGCCTATGCAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                     <  1:438872/138‑1 (MQ=255)

                                                                              cTTTATAGGCCTGGCGGGGTGCGCCACCGCTGACTTGCGCGGCGATTTTTGGGATGCGCGGCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTc                                                    <  3:268973/139‑1 (MQ=255)

                                                                               tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTca                                                   >  5:77522/1‑139 (MQ=255)

                                                                                 tatcctCCTGGCGGGTCTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCCCGGCCGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTc                                                    <  3:4571/131‑1 (MQ=255)

                                                                                 tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                     <  7:204999/135‑1 (MQ=255)

                                                                                 tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAAGGAAAAAAGCCCGCAACGCGGCCGTTTTACGGTTCCttgccttttgctggccttttgcgaaca                                                 >  4:142589/1‑134 (MQ=255)

                                                                                 tataGTCCTGTCGGGTGTCCCCACCTCTGCCTTGAGCGGCGATTTTTGGGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAACCGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC                                                  <  6:194629‑M1/138‑1 (MQ=255)

                                                                                 tataGGCCTGTCGGGTTTCGCCACCTCTTCCTTGAGCGTCTATTTTTGGGAGGCCGGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC                                                  <  6:16638‑M1/138‑1 (MQ=255)

                                                                                 tataGGCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGAGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                     <  8:174051/135‑1 (MQ=255)

                                                                                 tataGGCCTGTCGGGGTTCGCCACGTCTGTCTTGAGGGTGTTTTTTTGTGATGCTCTTATGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC                                                  <  2:164484‑M1/138‑1 (MQ=255)

                                                                                   taGTCCTGGCGGGTGTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG                                               <  1:266438‑M1/139‑1 (MQ=255)

                                                                                    aGTGCTGTGTGGTTTCGCCACCTTTGACTTGCGTTTCGATTTTTGTGATGCTGGTCAGGGGTGGGGAGCCTTTGTAACAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG                                               <  1:198686‑M1/138‑1 (MQ=255)

                                                                                     gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                       >  3:337295/1‑113 (MQ=255)

                                                                                     gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCACCAACGCGGCCTTTTTAAGGTTCCAGGACATTTTCTTGCCGTTTGCTCACATGtt                                             >  1:323630/1‑139 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGTTGGTCGTGAGGGGGGCGGGGGCTATGGAAAAACGCCCGCCACGCGGGCGTTTTACGGGTCCTGGCCGTTTGCTGGGCTTTTGCTGACATGtgggg                                          >  4:20140/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGGTGGGCGGGAGGGGGGCGGCGGCGATGGAAAAACGCCCGCAACGCGGGCGTTTTTCGGGTCCTGGCCGTGTTCTGGGCTTTTGGTAACAAGtgcgg                                          >  2:365724/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                  <  6:369914/99‑1 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACCTGTTcgg                                          >  8:336266/1‑137 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                          >  3:422827‑M1/1‑135 (MQ=255)

                                                                                        cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACCCCAGCAACCCGGCCGTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTTGTGACATGTTCtt                                          >  8:205264/1‑139 (MQ=255)

                                                                                         tGTCGGGTTTCGCCACCTCTGCCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGCGCCTATGGAAAAACGCCCGCAACGCGGCCGTTTTACGGTTCCGGGCCGTTTGCTGGCCGTTTGCTCACCAGTgcggc                                         >  4:268973‑M1/1‑134 (MQ=255)

                                                                                         tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                   >  2:259624/1‑97 (MQ=255)

                                                                                          gTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcg                                        <  7:298127‑M1/139‑7 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGTTTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcg                                        <  4:172099‑M1/138‑7 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCgg                                                                                                                       >  5:223665/1‑59 (MQ=255)

                                                                                           tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                     >  5:200121/1‑125 (MQ=255)

                                                                                                tttCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcgt                                  >  7:330570‑M1/1‑127 (MQ=255)

                                                                                                  tCGCCACCTCTGAGTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                 <  7:169175‑M1/138‑14 (MQ=255)

                                                                                                  tCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCCACGCGGCCATTTTACGGTTCCTGGCCGTTTGCTGGCCGTTTGCTCACATGTgcggcggggcgtgg                                >  5:331959‑M1/1‑125 (MQ=255)

                                                                                                   cGCCACCTCTGACTTGAGCTTCGATTTTTGTGATGCTCGTCAGGGGGGCTGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTTCTCACATGTgcggcggggcttgg                                <  8:304955‑M1/138‑15 (MQ=255)

                                                                                                   cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggc                               <  2:132888‑M1/139‑16 (MQ=255)

                                                                                                   cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttgg                                <  1:357075‑M1/138‑15 (MQ=255)

                                                                                                      cACCTCTGACTTGAGCGTCGTTTTTTGTTATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggc                            <  6:423780‑M1/139‑19 (MQ=255)

                                                                                                       acgtctgagTTGAGCGTCGATTTTTGTGATGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                           <  6:109273‑M1/136‑20 (MQ=255)

                                                                                                       aCCTCTGACTTGTGCGTCGTTTTTTGTGATGCTCGTCTGGGGGGCGGAGCGTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                           <  4:107541‑M1/139‑20 (MQ=255)

                                                                                                       aCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                           <  4:363020‑M1/139‑20 (MQ=255)

                                                                                                         ctctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                         <  3:74579‑M1/139‑22 (MQ=255)

                                                                                                         ctcgGACTTCAGCGTCGATTTTTGGGCTGCTCGGCAGGGGGGCGTAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcgcggcttggcggctta                         <  4:393479‑M1/139‑22 (MQ=255)

                                                                                                         cgctgaCTGGAGCGTCGATTTTTGTGATGCTGGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                         <  3:227516‑M1/137‑22 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                     <  2:57915‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                     <  5:360386‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                     <  2:330373‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                           <  4:53362‑M1/132‑20 (MQ=255)

                                                                                                              aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGAgcggcggggcttggcggcttattgt                     <  3:201112‑M1/138‑26 (MQ=255)

                                                                                                              aCTTGAGCGGCGATTTTTGTGATTCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                     <  8:27483‑M1/138‑26 (MQ=255)

                                                                                                               cTTGAGCGTCGATTTTTGTGATGCTGGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtat                   <  3:305420‑M1/139‑28 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGGCTATGGAAAAACGCCAGCAACGCGGCCTGTTTACGGTTCCTGGGCTGTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctttttgtatggc                >  4:42289‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggct               >  8:116187‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtat                   <  6:49261‑M1/135‑28 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                 >  6:395367‑M1/1‑108 (MQ=255)

                                                                                                                   aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCGGGCCTTTTGCTGGCCTTTTGCTCACCTGTgcggcggggcttggcggcggtttgtatggc                >  3:362002‑M1/1‑108 (MQ=255)

                                                                                                                        cGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTTCTCACATGTgcggcggggcttggcggcttattgtctgtctctta           >  1:100092‑M1/1‑103 (MQ=255)

                                                                                                                         gATTTTTGTGATGCTCGTCCGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgaca         <  8:3664‑M1/139‑38 (MQ=255)

                                                                                                                          aTTTTTGTGATGCTGGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgacac        <  7:395271‑M1/139‑39 (MQ=255)

                                                                                                                                         gTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                           >  5:170132/1‑41 (MQ=255)

GCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACC  >  pSIJ663_pHT315_araE_xy/5329‑5595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`