Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,287,928	G→T	10.4%	V141L (GTA→TTA)	U712_06535 →	Positive control factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,287,928	0	G	T	10.4%	204.1 / 2.5	77	V141L (GTA→TTA)	U712_06535	Positive control factor
Reads supporting (aligned to +/- strand):  ref base G (50/16);  new base T (2/8);  total (53/24)
Fisher's exact test for biased strand distribution p-value = 1.09e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGGACAGGTATCAGAAGAAGACAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACAC  >  CP006881/1287793‑1288063                                                                                                                                        |                                                                                                                                        gacggacAGGTATCAGAAGAAGACAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTa                                                                                                                                       >  1:179889/1‑138 (MQ=255)               agaagaagaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTg                                                                                                                        >  8:42254/1‑139 (MQ=255)                gaagaagaCAGAGATAGAATTCAAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGATCGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTg                                                                                                                        >  6:41123/1‑138 (MQ=255)                    aagaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAac                                                                                                                   >  2:29173/1‑139 (MQ=255)                    aagaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAac                                                                                                                   >  7:192589/1‑139 (MQ=255)                    aagaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAac                                                                                                                   >  6:25097/1‑139 (MQ=255)                    aagaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAac                                                                                                                   >  2:201225/1‑139 (MQ=255)                    aagaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGAAAac                                                                                                                   >  2:253872/1‑139 (MQ=255)                     agaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGT‑AAAAAATCGACAGTGCAAacga                                                                                                                 >  7:261934/1‑139 (MQ=255)                           agaTAGAATTCGTGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCTGAAAAAAATCTACAGTGCAAACGCCGAt                                                                                                             >  2:114770/1‑138 (MQ=255)                           agaTAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGAt                                                                                                             >  8:105884/1‑138 (MQ=255)                           agaTAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGAt                                                                                                             >  8:218348/1‑138 (MQ=255)                           agaTAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGT‑AAAAAATCGACAGTGCAAACGACGAtt                                                                                                            >  2:37010/1‑138 (MQ=255)                           agaTAGAATTCGAGAAGCATTAGCCATGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGACGATCTTCTCGGAGTAAAAAAATCGACAGTGCAAACGACGAt                                                                                                             >  5:34579/1‑138 (MQ=255)                              tAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGAGCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTaaa                                                                                                         <  3:216612/139‑1 (MQ=255)                                   ttCGACAAGCATTAGCCCTGTTACCGCACACACAAACGCAAATGTTTTTGCTCCATAAGGTCGAATGTTTTTCTTATGAACGGAGCGCCGATCTTCTCGGCGTAAAAAACTCGACAGTGCAAACGACGATTAAACGGGc                                                                                                    <  6:100964/139‑1 (MQ=255)                                      gagaAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCTAg                                                                                                 >  4:15423/1‑139 (MQ=255)                                            cATTAGCCTTTTTAACGTACAGATAAAAGTAAATGTTTTTGCTGAATAAGGTAGATTGTTTTTCTTATTAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTaaa                                                                                           <  5:400569/139‑1 (MQ=255)                                            cATTAGCCCTGTAACCGGACAGAGAAAAGGAAATGTTTTTGCGGCATAAGGTAGAATGTTTTTCTTATGAACGGAGCGCCGATCTTCTCGGCGAAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTaaa                                                                                           <  4:364891/139‑1 (MQ=255)                                                     tGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTAGGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCCACAGTGAAAACGACGATTAAACGGGCGATATTAAAGATGCa                                                                                     >  5:19215/1‑136 (MQ=255)                                                      gTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCATACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGa                                                                                 >  8:46898/1‑139 (MQ=255)                                                      gTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGa                                                                                 >  1:186125/1‑139 (MQ=255)                                                      gTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGa                                                                                 >  2:237333/1‑139 (MQ=255)                                                      gTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGa                                                                                 >  3:367019/1‑139 (MQ=255)                                                      gTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGa                                                                                 >  3:360998/1‑139 (MQ=255)                                                       ttAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGTATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGAGAGTTTAAAGATGCAAAGa                                                                                 >  6:80861/1‑138 (MQ=255)                                                             gACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGAcagcag                                                                           >  6:193859/1‑138 (MQ=255)                                                                 tagaAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCTTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCTAGTTTAAAGATGCAAAGACAGCAGgaac                                                                       >  1:219436/2‑137 (MQ=255)                                                                 gagaAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGgaag                                                                       >  8:154868/1‑138 (MQ=255)                                                                 gagaAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGgaag                                                                       >  5:314945/1‑138 (MQ=255)                                                                    aaatctaaaTGTTTTTGCTTGCTAAGGTAGATTTTTTTTATTATTAACGGATCCCCCATCTTGACGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAAt                                                                   <  7:93599/133‑1 (MQ=255)                                                                         gAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCTATCTGCTCGGCGT‑AAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCg                                                             <  8:261934/139‑1 (MQ=255)                                                                         gAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATc                                                              >  6:262273/1‑139 (MQ=255)                                                                            aTGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGa                                                                                 >  2:326254/1‑117 (MQ=255)                                                                            aTGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGa                                                                                 <  1:326254/117‑1 (MQ=255)                                                                            aTGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGATGAAATGAATCGAt                                                           >  4:442034/1‑139 (MQ=255)                                                                            aTGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGAt                                                           >  2:111386/1‑139 (MQ=255)                                                                            aTGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGAt                                                           >  1:20044/1‑139 (MQ=255)                                                                                 tttGCTCCATAAGTTAGATTGTTTTTATTATCACCGGATCGCCGATATTCTCGGCTTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACt                                                       <  5:376851/138‑1 (MQ=255)                                                                                   tgctgcATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGATTAAAGATGCAAAGACAGCAGGAAGAAATCAATCGATCACTTGc                                                    >  5:17751/1‑139 (MQ=255)                                                                                       gcATAAGGTAGAATTTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCCTAAAAAAATCGACAGTGCAAACGACGATTAAACGCGCGAGTTTAAAGATCCAATGACAGCTGGAAGAAATGAATCGATCACTTGCCTg                                                 >  1:409537/1‑138 (MQ=255)                                                                                        cATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCGAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGa                                                >  6:98527/1‑138 (MQ=255)                                                                                        cATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGctgtct                                               >  3:408390/1‑134 (MQ=255)                                                                                        cATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGa                                                >  1:140999/1‑138 (MQ=255)                                                                                          tacggcaGAATGTTTTGCTAATGACGGGAGCGCCGAGATCCACGCGTTAAAAAACTCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAg                                             <  3:263705/133‑1 (MQ=255)                                                                                           aaGGAATAATTTTTTTCTTATGACCGCAGCCCCGATCTTCGCGGCTAAAAAAAATCGACAGTGCACACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGc                                            <  5:25097/139‑1 (MQ=255)                                                                                            cggtttaaTTTTTTTTTTATTAAGGGATGTGCGATTTTATCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACTGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGc                                            <  1:253872/132‑1 (MQ=255)                                                                                                  aaTGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCa                                      >  5:29419/1‑138 (MQ=255)                                                                                                  aaTGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCa                                      >  3:76009/1‑138 (MQ=255)                                                                                                  aaTGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCa                                      >  3:57003/1‑138 (MQ=255)                                                                                                  aaTGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCa                                      >  6:253131/1‑138 (MQ=255)                                                                                                  aaTGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCa                                      >  6:157454/1‑138 (MQ=255)                                                                                                       ttttCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTATAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATCCGtt                                >  2:29581/1‑139 (MQ=255)                                                                                                       ttttCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGtt                                >  5:59629/1‑139 (MQ=255)                                                                                                         ttCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTg                              >  5:442105/1‑139 (MQ=255)                                                                                                         ttCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTg                              >  5:360715/1‑139 (MQ=255)                                                                                                         ttCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTg                              >  3:358629/1‑139 (MQ=255)                                                                                                         ttCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAACACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTg                              >  5:29787/1‑139 (MQ=255)                                                                                                          tattATCAACGGAGCGCCGATCTTCCCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTg                              <  7:42254/136‑1 (MQ=255)                                                                                                              aTGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCAcc                         >  3:97567/1‑139 (MQ=255)                                                                                                              aTGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCAcc                         >  7:384671/1‑139 (MQ=255)                                                                                                               tGAACGGATCGCCGAGCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCAcc                         <  4:216924/138‑1 (MQ=255)                                                                                                                 aagggATCGCCTATCTTCTGGGCTAAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                       <  3:15423/135‑1 (MQ=255)                                                                                                                   gggATGGCCGTTCTTCTCGGCTAAAAAAAATCTACTGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                    <  7:46898/138‑1 (MQ=255)                                                                                                                   cgtaTCGCCTATCTTCTCGGGTTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATAAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                    <  4:367019/136‑1 (MQ=255)                                                                                                                     gATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGt                  <  7:154868/139‑1 (MQ=255)                                                                                                                      aTCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGt                  >  1:217052/1‑138 (MQ=255)                                                                                                                          ccGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGaa               >  2:324342/1‑137 (MQ=255)                                                                                                                          ccGATCTTCGCGGCTAAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATa             <  4:57003/139‑1 (MQ=255)                                                                                                                          ccGAGCTCCTCGGCGTAAAAAAAACCCCCTCGCACACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATa             <  6:146373/139‑1 (MQ=255)                                                                                                                               cttctCGGCGTAAAAAACTCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa        <  2:287178/139‑1 (MQ=255)                                                                                                                               cttctCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa        <  7:105884/139‑1 (MQ=255)                                                                                                                                ttctGGGCTAAAAAAACTCCACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa        <  2:179889/138‑1 (MQ=255)                                                                                                                                  ctcGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGc                                            >  5:311755/1‑100 (MQ=255)                                                                                                                                  ctcGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGc                                            <  6:311755/100‑1 (MQ=255)                                                                                                                                  ctcGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa        <  6:314945/136‑1 (MQ=255)                                                                                                                                     ggCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGacac  >  7:316356/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GACGGACAGGTATCAGAAGAAGACAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGTTTAAAGATGCAAAGACAGCAGGAAGAAATGAATCGATCACTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACAC  >  CP006881/1287793‑1288063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑