Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,467,379 C→A 24.2% I20I (ATC→ATA)  U712_07540 → Putative efflux system component yknX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,467,3790CA24.2% 53.6 / 6.0 42I20I (ATC→ATA) U712_07540Putative efflux system component yknX
Reads supporting (aligned to +/- strand):  ref base C (19/12);  new base A (5/6);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATCATTCTATTCGGAATTTTAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCAAATGAA  >  CP006881/1467242‑1467505
                                                                                                                                         |                                                                                                                              
cAATCATTCTATTCGGAATTTTAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCa                                                                                                                               <  2:416484/139‑1 (MQ=255)
cAATCATTCTATTCGGAATTTTAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCa                                                                                                                               <  3:309929/139‑1 (MQ=255)
  aTCATTCTATTCGGAATTTTAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAat                                                                                                                             <  5:292149/139‑1 (MQ=255)
  aTCATTCTATTCGGAATTTTAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAat                                                                                                                             <  5:98683/139‑1 (MQ=255)
   tCATTCTATTCGGAATTTTAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGATGGAATCAata                                                                                                                            >  8:389954/1‑139 (MQ=255)
                     tAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTGTTTTTGTTGGTATAAAAATATAACGGTCTGCCGcgg                                                                                                          >  3:248296/1‑137 (MQ=255)
                     tAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCAGCta                                                                                                          >  3:352037/1‑138 (MQ=255)
                                             gTTTATTCAATTCTGTGGCGGGGGCATACGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGAAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGGCTGCCGCTCCGACAAGCGGCAGCGCCGGCAagg                                                                                  <  4:352037/139‑1 (MQ=255)
                                             gTTTAATCAATTCTGGGGCGGGGGCATACGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATAAATATATACCGGGCTGCCGCTCCGACACGCGGCAGCGCCGGCAagg                                                                                  <  1:71702/139‑1 (MQ=255)
                                                  aTCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCa                                                                                                                               >  1:418878/1‑89 (MQ=255)
                                                  aTCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCa                                                                                                                               <  2:418878/89‑1 (MQ=255)
                                                       ttCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTGTTTATGTTGTAATCAAAAAATAAACGACTTGCCCTCCGCCAAGCCGCAGCCGCCGCAAGAGGGTaaaacc                                                                        >  1:221479/1‑134 (MQ=255)
                                                            tgGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCGTTTTTTTGGAATAAATATATAAACGTAATCCGCTCAGACAATCGGCAGCCCCCGCAAGGACGTGAAGAAggg                                                                     >  3:58654/1‑137 (MQ=255)
                                                            tgGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCGGTTTTTGTTTGGATCAATATATAACGGTATGCCCCACCGCACAGCGGCAGCGCCCGCCAGGAGGTACCGACggggc                                                                   >  2:177656/1‑138 (MQ=255)
                                                            gggCGGGTGCATAACCACATGAAAAAAGCCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGGCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACggg                                                                     <  7:415524/136‑1 (MQ=255)
                                                                gggTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATCCCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGt                                                               <  5:331824/139‑1 (MQ=255)
                                                                 ggTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTTTTGGAATCAATATATAACGGTCTGCCGCTCCGACAAGCCGCAGCGCCGGCAAAGAGGGACAGACGGGGAGCGtt                                                              >  2:91043/1‑139 (MQ=255)
                                                                 ggTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCCGCGCCGGCAAGGAGGTTCAGACGGGGAGCGtt                                                              >  3:338677/1‑139 (MQ=255)
                                                                 ggTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCGTTTTGTTGGAATCAATATATACCGGTCTGCCCCGCCCACAAGCCGCAGCGCCGCCAAGGCGGTACAGACGGGGAGCGtt                                                              >  8:65902/1‑139 (MQ=255)
                                                                             cATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGa                                                   >  6:99111/1‑138 (MQ=255)
                                                                             cATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGa                                                   >  6:125676/1‑138 (MQ=255)
                                                                             cATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATAACGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGa                                                   >  6:213518/1‑138 (MQ=255)
                                                                             cATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCGCTTTTTGTTGGCATCAATATATACAGGTCTGCCGCTCCGACCAGCGGCCGCGCCGGCAAGGCGGTACAGCCGGGGCGCGGGGAAGAAAAAGat                                                  >  4:92446/1‑138 (MQ=255)
                                                                              aTGAAAAAAGTCTGGATAGGAATTGGAATCGCGGTTATAGTCGCGCTTTTTGTTGGTATAAATATATAACAGTATTCCGCTCCGACAACCGGCAGCGCCGTCAAGGAGGTACATCCGGGGAGCGTTGAAGAAAATGaa                                                  >  7:423587/1‑138 (MQ=255)
                                                                               tGAAAAAAGTCTGGATCGGAATTGGATTCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCGCCGCCAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGCGCGGTGAAGAAAATGaaa                                                 >  2:356989/1‑138 (MQ=255)
                                                                               tGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCGTTTTGTTGTAATAAATATATACCGGTCTTCAGCGCAGACAAGCGGCCGCGCCGGCAAGGAGGTACAGCCGGGGAGCGTTGAAGAAAATGAAAt                                                >  8:106984/1‑139 (MQ=255)
                                                                                        tCTGTATCGTAATTGGAATGGATTTTATAGTAGCTCTTTTTGTTGGAATCAATATATACAGGTCTGACGCTCCGACACGCGGCAGCGCCGGCAAGTAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAAc                                       <  7:335194/139‑1 (MQ=255)
                                                                                                       gAATCGCAGTTATAGTCGCTGATTTTGGTGGAATAAATATATACCCGTCTGCCGCACCGACAATCGGCAGCGCCGGCAACGAGGTACAAAAGCGGACCGGTGAATAAAATGAAATCTCATCAACCGGTAAGGTTCCTgg                        >  8:186519/1‑139 (MQ=255)
                                                                                                            gCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTgg                        >  8:433902/1‑134 (MQ=255)
                                                                                                            gCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTgg                        <  7:433902/134‑1 (MQ=255)
                                                                                                                  ataGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAAt             >  7:108896/1‑139 (MQ=255)
                                                                                                                    aGTCGCTTTTTTTTTTGTAATAATTTTCTACCGGTCGGCCGGCCCCACAGGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAAtt            <  7:186519/138‑1 (MQ=255)
                                                                                                                        gcgttttttGTGTCAATAAATATATCCCGGTCGGCCGCGCCGACATGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCaa       <  4:168539/135‑1 (MQ=255)
                                                                                                                        gCTGTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCa        >  6:114907/1‑138 (MQ=255)
                                                                                                                        gCTGTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAACGAGGTTCAGACGGGGAGCGGTGAAGAAAATGAAATATCATCAACGGTTATTGTTCCTGGCACGCTAAAATTTTCa        >  3:132059/1‑138 (MQ=255)
                                                                                                                        gCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCa        >  5:342061/1‑138 (MQ=255)
                                                                                                                         ctctTTTTGTTGTAATAAATATATCCCGGGCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCaaa      <  3:407016/139‑1 (MQ=255)
                                                                                                                          tctTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCAAAt     >  3:422763/1‑139 (MQ=255)
                                                                                                                          gatttttGTTGTATTCAATATATACGGGTCTGCGGCGGCAACAAGGTTCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTGCCTGGCACGCTAAAATTTTCaa       <  7:27469/135‑1 (MQ=255)
                                                                                                                              tttGTTGTAATAAATATATCCCGGTCGGCCGCTCCGACACGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCAAATg    <  4:338677/136‑1 (MQ=255)
                                                                                                                              tttGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCAAATGaa  <  5:125676/138‑1 (MQ=255)
                                                                                                                              tttGTGGTAATAAATATAACCGGGGCGGCCGCCCCCACACGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCAAATGaa  <  3:243791/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                              
CAATCATTCTATTCGGAATTTTAGTTGTATTTTCGCTTTTTTCAGGTTTAATCAATTCTGTGGCGGGTGCATAAGCTCATGAAAAAAGTCTGGATCGGAATTGGAATCGCAGTTATAGTCGCTCTTTTTGTTGGAATCAATATATACCGGTCTGCCGCTCCGACAAGCGGCAGCGCCGGCAAGGAGGTACAGACGGGGAGCGTTGAAGAAAATGAAATCTCATCAACGGTTATGGTTCCTGGCACGCTAAAATTTTCAAATGAA  >  CP006881/1467242‑1467505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑