| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 66,157 | T→G | 19.2% | V447V (GTT→GTG) | U712_00295 → | putative membrane protein yabM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 66,157 | 0 | T | G | 19.2% | 92.7 / 4.3 | 48 | V447V (GTT→GTG) | U712_00295 | putative membrane protein yabM |
| Reads supporting (aligned to +/- strand): ref base T (9/29); new base G (8/2); total (17/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTAAACACACTTTTGGTTCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGAGAGTCTTTCTGCTGTTGCCATTGGTGG > CP006881/66021‑66294 | tgtTAAACACACTTTTGGTTCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGtttt > 3:379875/1‑139 (MQ=255) tAAACACACTTTTGGTTCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGAt > 2:125059/1‑139 (MQ=255) tAAACACACTTTTGGTTCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGTTGAt > 2:118463/1‑139 (MQ=255) acTTTTGGTTCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGatt > 2:356441/1‑139 (MQ=255) ttGGTTCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGatta‑t < 6:133129/137‑1 (MQ=255) ttCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTT‑TTTGATc > 4:340290/1‑139 (MQ=255) cgctCTATGGGATTGTAGGAGCATCTCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCTGCAAAATGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTg < 5:356259/138‑1 (MQ=255) cgcgCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTg < 6:406891/138‑1 (MQ=255) cgcgCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTg < 1:95993/138‑1 (MQ=255) cgagcTATGGGATTGAAGGCGCATCGCTTGCGTCAGCGGCTTCCTTTGCGGCTGGCGCCTGCCTTTAATTGTTTCATTTTCTGCATTAGGAATGGCTCGATAAGCTTTGAGGCGTGTTGACTCCGATTA‑TTGGATCTg < 4:427650/135‑1 (MQ=255) aaGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGTTTAGGCTTA‑TTTGATCTGCTCTTTTGAtttgagg > 1:391309/1‑133 (MQ=255) aaGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGTTGGTTGGGGTTA‑TTGGTTCTGCTCTTTTGATGTGAgg > 7:388671/1‑138 (MQ=255) aaGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGTTGATTACGATTA‑TTGGATCTGGTCTTTTGATGTCAGc > 6:211503/1‑139 (MQ=255) aTCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTGCGATTT‑TTTGATATGGTCTTTTGATGTCAGCTGTTttg > 8:394062/1‑139 (MQ=255) tCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTttgc < 7:38131/139‑1 (MQ=255) tCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTttgc < 3:145475/139‑1 (MQ=255) tCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTttgc < 7:65763/139‑1 (MQ=255) gCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGTTGCTTGCGATTA‑TTGGGTGTGGTCGTTTGATGTCAGGTGGTTTGGTTGGGt > 1:289668/1‑139 (MQ=255) aGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATAcg < 5:364896/139‑1 (MQ=255) aGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATAcg < 7:394062/139‑1 (MQ=255) aGCGGCGTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTGCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATAcg < 1:149302/139‑1 (MQ=255) aGCGGCGTCCTTTGCGGCTGTCGCCGGCCGTAACTTGTATCCGCTGCGGCAACAGGAATGGCTCGCTAAGCTTAGAGGCGTTTTGATTACGATTA‑TTGGATCTGCTCTTTTGATGGCAGCTGTTTTGCGGGCGTATAcg < 4:226457/139‑1 (MQ=255) tCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGTTGTTTGCGTATT‑TTTGTTGTGGTCTTTTGTTGTGAGCGGGTTTGGTTGGGGATAggcgggggt > 3:295892/1‑134 (MQ=255) tGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGa < 5:211503/137‑1 (MQ=255) tGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGa < 6:202068/137‑1 (MQ=255) tGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGa < 1:356441/137‑1 (MQ=255) tGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGa < 8:90239/137‑1 (MQ=255) tGCGGCGGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGa < 6:337320/137‑1 (MQ=255) tGCGGCGGGCGCCGGCCTTTACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGGTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGGCTCTGGa < 7:111503/137‑1 (MQ=255) gTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGTTTGCGATTA‑TTGGTTCTGGGCGTTTGATGTGAGCTGTTTTTCTTTGGTATTAGCGTCTCTGGGCTTtgttgt > 7:298100/1‑139 (MQ=255) cGGCCTTAACTTGTATCAGCGTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTGCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTGGCGTATACGCGTCTCTGGACTTTCTTGTATCCt < 3:103578/139‑1 (MQ=255) ccTTAACTTTTATCAGCTTCGTCAATAGGAATGGCTCGATATGATTAGAGGCGTGTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTTTTTTGCTTGCTTTTACGCGTCTCTGGACTTTCTTGTTTGCTGCa < 2:8832/139‑1 (MQ=255) ccTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCa < 7:407844/139‑1 (MQ=255) gTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGATGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAag < 3:37509/138‑1 (MQ=255) tATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTTATTACGATTA‑TTTTTTGTGGGATTTTTTTTTGATGTGGTTTTGTTTCGTATACCCGTCTATGTACTTTTATTTTTTATTCAACAGGAagag > 4:64598/1‑139 (MQ=255) tATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGTTGTTTGCGATTT‑TTTGGTATGCTGTTTTGATGTGAGCTGTTTTGCTTGGGGATACGCGTGTCTGGGCGTTGTTGTTTCCTGCGAAAGGAagag > 5:128029/1‑139 (MQ=255) tATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGGTGATTAAGATTATTTGGAT‑TGCTCTTTTGATGTGAGGAGTTTTTCTTGCGTATTCTCTGGGCTGGTGTGTCTTGTTTCATGCAACGGGAagag > 8:240600/1‑139 (MQ=255) cTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTGGTTTTTACGATTA‑TTTGATCGGGTGTTTTTTTGTAAGCGGGTTTGGTTTGGTTTACTCGGCGCGGGTCTTTGTTTTTTGCTGGAACAGGAAGAGGcgcgc > 6:279626/1‑138 (MQ=255) ggCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTTGAt > 6:178556/1‑54 (MQ=255) ggCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGAt < 5:178556/54‑1 (MQ=255) aaTGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGagag < 3:177293/139‑1 (MQ=255) aTGTCTCTATAATCTTAGAGGCGTTTTGATTCCTTTTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGGGTATCAGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGagag < 3:64598/138‑1 (MQ=255) cTCGATAAGCTTAGAGGCGTTTTGATTCGGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTTCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGAGAGTCttt < 3:325022/139‑1 (MQ=255) gATACGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGAGAGTCTTTctg < 1:118463/139‑1 (MQ=255) gATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCGGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGGGATTGAGAGTCTTTctg < 1:338105/139‑1 (MQ=255) gATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGGCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGAGAGTCTTTctg < 1:125059/139‑1 (MQ=255) ttaGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATATGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGAGAGTCTTTctgct < 7:240600/137‑1 (MQ=255) tAAGCTTAGAGGCGTTTTGATTGGGATTT‑TTGGAGGTGCTGTTTTGTTTTAAGCGGTTTTTGTTGGGTATACGCGGGGGTGGACTTTCTTGTTTGCTGGGACAGGGAGTGGGGGGGCGGGGGTTGAGAGGCTTTctggg > 6:105913/1‑137 (MQ=255) tAAGATTAGAGGCGTTTTGATTCCGATTA‑TTGTATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGAATTTCTTGTTTCCTGCAACAGCAAGAGGAGCGGCTGTGATTGAGAGTCTTTctgct < 1:14828/139‑1 (MQ=255) ttttGATTCCGATTA‑TTTGTTCTGCTGTTTTTTTTTGAGCGGATTTGCTTGCGTATAAGCGTCTCTGGGCATTATTGTTTACTGCAACACGAATAGGAGCGGCGGTGATTGAGAGTCTTTCTGCTGTTGCGATtggtgg > 8:105571/1‑139 (MQ=255) | TGTTAAACACACTTTTGGTTCCGCGCTATGGGATTGAAGGCGCATCGCTTGCGACAGCGGCTTCCTTTGCGGCTGTCGCCGGCCTTAACTTGTATCAGCTTCGGCAAAAGGAATGGCTCGATAAGCTTAGAGGCGTTTTGATTCCGATTA‑TTGGATCTGCTCTTTTGATGTCAGCTGTTTTGCTTGCGTATACGCGTCTCTGGACTTTCTTGTTTCCTGCAACAGGAAGAGGAGCGGCTGTGATTGAGAGTCTTTCTGCTGTTGCCATTGGTGG > CP006881/66021‑66294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |