| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 1,690,479 | A→G | 7.5% | E103E (GAA→GAG) | U712_08705 → | DNA polymerase III polC‑type |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,690,479 | 0 | A | G | 7.5% | 165.8 / 3.4 | 58 | E103E (GAA→GAG) | U712_08705 | DNA polymerase III polC‑type |
| Reads supporting (aligned to +/- strand): ref base A (24/27); new base G (2/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
TTTTCAAATATATGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAACAAATACAGTTCCAT > CP006881/1690341‑1690613 | ttttCAAATATATGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTgaa < 5:321632/139‑1 (MQ=255) ttCAAATATATGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTgaag < 5:359918/138‑1 (MQ=255) ttCAAATATATGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTgaag < 4:439026/138‑1 (MQ=255) ttCAAATATATGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATATTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTgaa < 5:98311/137‑1 (MQ=255) cAAATATCTGCCACATTAACCACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTgaagaa < 8:195226/138‑1 (MQ=255) tatatGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCa < 5:404489/139‑1 (MQ=255) tGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAggg > 6:433924/1‑138 (MQ=255) tAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAAGTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCACGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTcgcc > 8:38640/1‑139 (MQ=255) aacGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGccgccg < 6:34200/139‑1 (MQ=255) aGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAGGAACTGCAGGGCATTTCGCCGCCGATTa > 2:63120/1‑139 (MQ=255) aGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTa > 6:113305/1‑139 (MQ=255) aGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTa > 2:295679/1‑139 (MQ=255) aGGCTCACGCAATCGTTTGCCCACATAGCAAAACTGACATCTACAATTCAACTTCAGGATGCCGACGTCAGTGAAAGTATCGTTCACGACTACTGGACCCGCTGCATTGATGAACTGCAGGGCATTTCGCCGCCGAtt > 1:345592/1‑138 (MQ=255) cTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCa < 7:268262/139‑1 (MQ=255) aTCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAGGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCtttt > 6:137188/1‑138 (MQ=255) tCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTa > 1:140135/1‑138 (MQ=255) tGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAg > 8:32417/1‑139 (MQ=255) aTAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGTACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAAc > 6:391777/1‑139 (MQ=255) aTAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAAc > 6:214441/1‑129 (MQ=255) aTAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAAc < 5:214441/129‑1 (MQ=255) aTAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAAc > 5:89026/1‑139 (MQ=255) aTAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAAc > 1:144775/1‑139 (MQ=255) aTAGCAAAAGTGACATCATCAATTGAAGTTCAGGATGCCGAGGTCTGGGAAAGTAGCGTTGAAGACTACTGGGCACGGTGCATTGATGAACTGCAGGGCATATCGCCGCCGATTATCAGTCTATTAAACCAGCACAAAc < 6:193988/139‑1 (MQ=255) gCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAGGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCg < 1:63120/138‑1 (MQ=255) gttaCATCTTCAATTGAAGTTAAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGCGCAGTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAACACCCAAGCTg > 4:434745/4‑138 (MQ=255) tGACATATTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGaa < 1:293296/139‑1 (MQ=255) gTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTg > 4:99426/1‑139 (MQ=255) cAGGCTGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACTCTGCATTGAAGAACTGCAGGGCAGTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTc < 1:95937/138‑1 (MQ=255) cAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTc < 4:18869/138‑1 (MQ=255) ccGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTcaaaaccaa < 5:113305/139‑1 (MQ=255) cGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCTTTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTcaaaaccaa > 1:104939/1‑138 (MQ=255) acagcAGTTAAAGTCTCGTTCAATACTACTGGTCACTCTTCATTGAATAACTGCATGGCATTTCGCCGCCGATTATCAGTCTTTTATACCATCAAAAACCTAATATTAAGGGCAATAAACTGATTGTcaaaaccaaaac < 7:310205/135‑1 (MQ=255) aGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGAt < 2:144775/138‑1 (MQ=255) aGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATa > 7:263164/1‑139 (MQ=255) tCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGc > 5:400006/1‑139 (MQ=255) tCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGAAAAAACCGAATCTTAAGGGCAATAAACTGATTGTCAAAAACAAAACAGATAAATAAGctgt > 8:310205/1‑136 (MQ=255) gACTACTGGTCACGCTTCATTGAGTAACTGCAGGGCATTTCGCCGCCTATTATCAGTCTTTTAAACCACCAAAAACCGAAGCTGAAGGGAAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGTCTGCGCTaa < 5:137188/139‑1 (MQ=255) gACTACTGGTCACGCTGCATTGATGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTaa < 8:427396/139‑1 (MQ=255) gACTACTGGTCACGCTGCATTGAAGACCTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTaa < 1:283535/139‑1 (MQ=255) gACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTaa < 2:140135/139‑1 (MQ=255) gACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTaa < 6:400006/139‑1 (MQ=255) gACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTa > 7:324546/1‑138 (MQ=255) gACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCAATTCGCCGCCGATTATCAGTCTTTTAAACAAGCAAAAACCGATGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTaa < 7:363264/139‑1 (MQ=255) ctactGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCACCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACAAAAACAGATACAGAAGCGGCTGCGCTAAAg < 1:295679/139‑1 (MQ=255) actGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGaa > 7:258491/1‑139 (MQ=255) ctGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCgg > 1:3675/1‑125 (MQ=255) ctGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCgg < 2:3675/125‑1 (MQ=255) ctGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTg < 3:339975/128‑1 (MQ=255) ctGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTg > 4:339975/1‑128 (MQ=255) ctGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGa < 3:99426/137‑1 (MQ=255) ctGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAAc < 7:32417/139‑1 (MQ=255) ctGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAAc < 8:263164/139‑1 (MQ=255) ctGGTCACGCTGAATTGAATAACTGCAGGGCATTTCCCCGCCGATTATCAGTCTTTTAAACCAGCAAAACCCGAAGCTGACGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAAc < 5:34100/139‑1 (MQ=255) cACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACTGAAGCGGCTGCGCTAAATAACaaaa > 3:51949/1‑137 (MQ=255) cACGCTGATTTGAAGAACTGCTGGGCATTTCGCCGCCTATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAACAAATa < 8:258491/139‑1 (MQ=255) aCGCTGCATTGAAGTACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAACAAATa < 5:391777/138‑1 (MQ=255) ggagaaTTGAAGAAAGGCAGGGCATTTCGCCGCCGTTTATCAGTCTTTTAAACATGCAAAATCCGTTGCTGATGGGCAATATACTTATTGGCTAAACCAAAACCGTAAGCCCAGGGTCTGCGCTAAAGAACTAATTCAg < 2:345592/134‑1 (MQ=255) gCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCGAAAGAACAAATACAg > 4:124354/1‑139 (MQ=255) cATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAACAAATACAGTTcc < 5:433924/139‑1 (MQ=255) aTTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCaa > 7:368554/1‑74 (MQ=255) aTTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCaa < 8:368554/74‑1 (MQ=255) aTTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCaa < 4:323431/74‑1 (MQ=255) aTTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCaa > 3:323431/1‑74 (MQ=255) ttcaagaagTGCAGGGAATTTCGCCGCCGATTATCAGTATTTTAAACCAGCAAAAACCGAAGCTTAAGGGCAATAACCTGATTGACAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAACAAATACAGTTCCAt < 6:42803/136‑1 (MQ=255) | TTTTCAAATATATGACACATTAACAACGAGGCTGACGCAATCGTTTGCCCACATAGCAAAAGTGACATCTTCAATTGAAGTTCAGGATGCCGAGGTCAGTGAAAGTATCGTTCAAGACTACTGGTCACGCTGCATTGAAGAACTGCAGGGCATTTCGCCGCCGATTATCAGTCTTTTAAACCAGCAAAAACCGAAGCTGAAGGGCAATAAACTGATTGTCAAAACCAAAACAGATACAGAAGCGGCTGCGCTAAAGAACAAATACAGTTCCAT > CP006881/1690341‑1690613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |