| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 72,897 | G→A | 6.0% | R790H (CGC→CAC) | U712_00330 → | Stage II sporulation protein E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 72,897 | 0 | G | A | 6.0% | 213.5 / 3.0 | 67 | R790H (CGC→CAC) | U712_00330 | Stage II sporulation protein E |
| Reads supporting (aligned to +/- strand): ref base G (32/31); new base A (2/2); total (34/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01 | |||||||||||
TGATGAGTGATGGCATTTTTGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCTTCCGTAT > CP006881/72765‑73032 | tgatgaGTGATGGCATTTTTGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGcac > 8:28442/1‑136 (MQ=255)tgatgaGTGATGGCATTTTTGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGcac > 3:27387/1‑136 (MQ=255)tgatgaGTGATGGCATTTTTGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGcac > 3:303293/1‑136 (MQ=255) gaGTGATGGCATTTTTGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATc < 4:415725/138‑1 (MQ=255) gCATTTTTGAGGGGCCTAAGCATGTGGAAAATCCCGACTTATGGATGAGGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAAt > 2:73329/1‑139 (MQ=255) aTTTTTGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTg < 5:71484/139‑1 (MQ=255) tttGCGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 4:223601/138‑1 (MQ=255) tttGCGGGGCCTAAGCATGTGGAAAAACACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 5:383150/138‑1 (MQ=255) tttGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACATATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 4:331598/138‑1 (MQ=255) tttGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 1:93480/138‑1 (MQ=255) tttGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 5:441797/138‑1 (MQ=255) tttGAGGGGCATAAGCATGATGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 5:114096/138‑1 (MQ=255) tgcggggCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGa < 3:68265/135‑1 (MQ=255) tGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGa < 5:348370/138‑1 (MQ=255) gCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACTatat > 1:17094/1‑138 (MQ=255) tgtgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACtgttgtt < 4:134048/139‑1 (MQ=255) tgtgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACtgttgtt < 5:295838/139‑1 (MQ=255) tgGAAAATCACGACTTATGGATGTAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCACACAAGATCCGGCCAAATTGAGGACGATATGACtgttgttgt > 7:368517/1‑139 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 5:43964/119‑1 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg > 6:43964/1‑119 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGatat > 3:429923/1‑127 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGatat < 4:429923/127‑1 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACtgttgttgt > 6:211387/1‑139 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACtgttgttgt > 6:249600/1‑139 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACtgttgttgt > 8:56952/1‑139 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACtgttgtggt > 4:265199/1‑139 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCAGCCGCACAAGATCCGGCCAAATTGAGGACGATATGACtgttgttgt > 7:144636/1‑139 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGAGTGCCGATTTGCTTATGGAAGAAGTCATCCACACAAGATCCGGCCAAATTGAGGa > 3:227553/1‑121 (MQ=255) tgGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGACTGCCGATTTGCTTATGGAAGAAGTCATCCACACAAGATCCGGCCAAATTGAGGa < 4:227553/121‑1 (MQ=255) cACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGa < 1:187104/139‑1 (MQ=255) aCGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCTGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGa < 3:425259/138‑1 (MQ=255) aCTTATGGAAGCAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGa < 6:252277/139‑1 (MQ=255) cTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGAt < 3:398180/139‑1 (MQ=255) cTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGAt < 7:28442/139‑1 (MQ=255) cTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGAt < 7:297272/139‑1 (MQ=255) cTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGAt < 2:291688/139‑1 (MQ=255) cTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGAt < 5:211387/139‑1 (MQ=255) cTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGAt < 4:303293/139‑1 (MQ=255) taGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCACACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATaca < 8:368517/137‑1 (MQ=255) aaGCGTAAAATGAAAGGGTTGAAAACAAATGCCCCGCAGGAGATTGCCGAGTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAATTTGAGGACGATATTCCTGGTGGTGTCGTCCGGAGTGATCCCAATacac > 8:315847/1‑139 (MQ=255) aaGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATacac > 6:213858/1‑139 (MQ=255) aaGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTGGCTTATGGAAGAAGTCATCCGCACAAGATCCGGGCAACTTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGCTGACAATacac > 8:321733/1‑139 (MQ=255) aGCGTAAAATGCAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATcaca < 1:211835/132‑1 (MQ=255) aGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATcaca > 2:211835/1‑132 (MQ=255) aGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATca < 8:201346/130‑1 (MQ=255) aGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATca > 7:201346/1‑130 (MQ=255) aaaTGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTg < 7:54811/139‑1 (MQ=255) aaaTGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTg > 7:323168/1‑139 (MQ=255) aaaTGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATAGAGGACGATATGAATGTTGTTGTCGTCCGGATTGATCACAATACACAGAAgag > 8:294683/1‑137 (MQ=255) aaTGAACGGGGTGAACACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACACGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGGCCGGATTGATCACAATACACCGAAGTgg < 6:258488/139‑1 (MQ=255) aaTGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTgg < 5:249600/139‑1 (MQ=255) aaGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg > 4:155561/1‑83 (MQ=255) aaGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAg < 3:155561/83‑1 (MQ=255) aaGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGCTCACAATACACCGCAGTGGGCGTc > 1:205111/1‑139 (MQ=255) aaGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATc > 5:134177/1‑139 (MQ=255) aaGGGGTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATc < 7:437601/139‑1 (MQ=255) aTGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCgg > 5:140175/1‑138 (MQ=255) tGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAaaacaaa > 4:126521/1‑139 (MQ=255) ccGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAaaacaaac > 5:408142/1‑138 (MQ=255) gaagaaGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATac < 6:408142/138‑2 (MQ=255) aagTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCtt > 8:420097/1‑139 (MQ=255) aagTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCtt > 4:8641/1‑139 (MQ=255) aTCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGTGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCTTCCGta > 4:200175/1‑139 (MQ=255) aTCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCTTCCGta > 2:205572/1‑139 (MQ=255) aTCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCTTCCGta > 6:323203/1‑139 (MQ=255) aTCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCGTCCGta > 1:342097/1‑139 (MQ=255) ccGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCTTCCGtat > 4:2704/1‑138 (MQ=255) | TGATGAGTGATGGCATTTTTGAGGGGCCTAAGCATGTGGAAAATCACGACTTATGGATGAAGCGTAAAATGAAAGGGTTGAAAACAAATGACCCGCAGGAGATTGCCGATTTGCTTATGGAAGAAGTCATCCGCACAAGATCCGGCCAAATTGAGGACGATATGACTGTTGTTGTCGTCCGGATTGATCACAATACACCGAAGTGGGCATCCATTCCTGTTCCGGCAATCTTTCAAAACAAACAAGAAATTTCATAACGCTTCCGTAT > CP006881/72765‑73032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |