Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,107,527 A→C 23.8% G111G (GGT→GGG)  U712_10490 ← putative multidrug resistance protein ypnP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,107,5270AC23.8% 79.9 / 7.8 48G111G (GGT→GGG) U712_10490putative multidrug resistance protein ypnP
Reads supporting (aligned to +/- strand):  ref base A (29/7);  new base C (2/10);  total (31/17)
Fisher's exact test for biased strand distribution p-value = 1.35e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGCCCAAAATA  >  CP006881/2107402‑2107665
                                                                                                                             |                                                                                                                                          
cttaTAAATTTATAACCAAACAAAAAAAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGATAAAAAAACCGGCCGCGCCGa                                                                                                                               <  7:370702/136‑1 (MQ=255)
 tGATAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGaa                                                                                                                              >  7:337069/1‑139 (MQ=255)
 tGATAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGaa                                                                                                                              >  1:86933/1‑139 (MQ=255)
   aTAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATc                                                                                                                            >  3:382816/1‑139 (MQ=255)
   aTAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATc                                                                                                                            >  4:66571/1‑139 (MQ=255)
   aTAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATc                                                                                                                            >  5:345299/1‑139 (MQ=255)
   aTAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATc                                                                                                                            >  6:208976/1‑139 (MQ=255)
   aTAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCGCTGAGAGAAAAAAACCGGCCGCGCCGAATc                                                                                                                            >  1:133072/1‑139 (MQ=255)
   aTAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCAGGCTTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATc                                                                                                                            >  8:126558/1‑139 (MQ=255)
   aTAAAGTTAGAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCTGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAGGACGCAGCAAAAGCTCTGAGAGAATAAACACGGCCGCGCCGAATc                                                                                                                            >  4:419320/1‑139 (MQ=255)
        gTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATg                                                                                                                       >  6:327383/1‑139 (MQ=255)
        gTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATg                                                                                                                       >  5:228785/1‑139 (MQ=255)
           ataACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTATAAGACGCAGCAAAAGCGCTGAGAGAAAAAAACCGGCTGGGCCGCATCCGCTGCtt                                                                                                                    >  2:379376/1‑139 (MQ=255)
             aaCCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGCATCCGCTGCttgg                                                                                                                  >  3:254280/1‑137 (MQ=255)
             aaCCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCGCTGAGAGAAAAAAACCGGCCGCGCCGCATCCGCTGCTTAg                                                                                                                  >  6:16087/1‑139 (MQ=255)
                  aacaaaAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCGTAGGCCTg                                                                                                             >  7:85532/1‑139 (MQ=255)
                   acaaaAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCATCAAGCGGTATCATTGACTCCGGCGTTTTTAGATGACGCAGCCAAAGCTCTGAGAGATAAAAACCGGCCGCGCCGACTCCGATGCTTAGGCCTGt                                                                                                            >  6:332955/1‑139 (MQ=255)
                   acaaaAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGCAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGt                                                                                                            >  8:151256/1‑139 (MQ=255)
                   acaaaAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGt                                                                                                            >  1:232056/1‑139 (MQ=255)
                   acaaaAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAAACCGATGCATAGGCCTGt                                                                                                            >  5:323413/1‑139 (MQ=255)
                       aaataaaatGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGCCTCCGTCGTGTTTAGAAGCCGCAGCAAACGCTAGGAGAGACAAATCACGGCCGCGGCGAATCCAATGCTTAGGCCTGtgatt                                                                                                        >  4:236782/1‑135 (MQ=255)
                                 cataTAAATTTATTATGAAAATCGGTCTCAGCATGGGGTATCATTGAGTCCGGCGTTTTTATAAGCCGCAGCAAAAGCGTTGACAAAAAAAACCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAAcaacaa                                                                                              <  8:85532/136‑1 (MQ=255)
                                  ctaTAAATTTAATCTTTAAATACTTATCAGCAAGCGTTATAATTGACTCCGGCTTTTTTATAATACCAAGAAAAAGCTATGACACAAAAAACCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACaaa                                                                                             <  1:267689/137‑1 (MQ=255)
                                       aaTTCAATCGGTAAATAGGTCCCACCAAGCGGAATCTTTGACTCGGGCGTTTTTAGACGCCGCAGCAAAAGCTCTGAGAGAAAAACACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGt                                                                                        <  8:346352/139‑1 (MQ=255)
                                           taaTGTGAAAATACGGCTCACCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGCCGCACAAAACGCGCTGAGATAAAAAACCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTaa                                                                                     <  6:345299/137‑1 (MQ=255)
                                              tCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAataa                                                                                  >  2:58691/1‑137 (MQ=255)
                                                  tAAATCCGTCTCAGCACGCGTTATCATTGCCCCCGGCGTTTTTACAAGCCCCACCAAACCCTCTGAGACAAAAAACCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATac                                                                                <  1:48246/136‑1 (MQ=255)
                                                  tAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCCATGCTTAGGCCTGTTTATAAAACAACAAAATCGGTTATAAacgac                                                                             >  3:4666/1‑139 (MQ=255)
                                                        cGTCTCAGCACGGGGTATCATCGCCTCCGGGTTTTTTACAAGCCCCAGCAAAAGCGCTGATATAAAAAACCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCAt                                                                        <  2:86933/138‑1 (MQ=255)
                                                        cGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCAt                                                                        <  5:155161/138‑1 (MQ=255)
                                                          tctcAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCGTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTcc                                                                     >  1:249985/1‑139 (MQ=255)
                                                              aGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCttt                                                                  >  3:125853/1‑138 (MQ=255)
                                                                 acgCGTTATCATGGCCTCCGGCGTTTTTCCAAGCCCCACCAAACGCTCGTCAACAAAAAACCCTGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAACGCGTTAATATACGACGCCATTCCTTTtata                                                              <  2:133072/137‑1 (MQ=255)
                                                                   gCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGCGAGAAAAAAACCGGCCGCGCCGAATCCGATGCGTAGGCCTGTTAATAAAACAACAAAAGCGTGAATATACGACGCCATTCCTTTTatat                                                             >  1:284272/1‑138 (MQ=255)
                                                                        aTCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCtt                                                       >  4:371849/1‑139 (MQ=255)
                                                                               aCTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAACGCGTTAATATACGACGCCATTCATTTTATATCATCGTTTGCttt                                                >  6:1343/1‑139 (MQ=255)
                                                                               aCTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCAGAGAGAAAAAAACCGGCCGCGCCGAATCCGCTGCTTAGGCCTGTTAATAAAACAACAAAATCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCttt                                                >  3:417792/1‑139 (MQ=255)
                                                                                 tCCGGCGTTTTTAGAAGACGCCGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGCATCCGCGGGTTTGGCCTGGTAAAAAAACAACAAACGCGGTAATATACGCCCCCAATCCTTTTATATAATCTTTTTCgtttt                                              >  6:254049/1‑139 (MQ=255)
                                                                                 tCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGCATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCttttt                                              >  8:282914/1‑139 (MQ=255)
                                                                                   cGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCCGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGAGTTAATATACGACGCCATTCCTTTTATATAATATTTTCCTTTTTGc                                            >  3:292091/1‑139 (MQ=255)
                                                                                             ttaagaCGCAGCAAATCCTCTTACAGAAAAAAACCGGCCGCGCCGAATCCGACGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATaaa                                   <  5:208976/136‑1 (MQ=255)
                                                                                                 gaCGCAGCAAAAGCGCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGt                              <  8:136613/139‑1 (MQ=255)
                                                                                                      cgcaAACGCTCTGAGACAAAAAACCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACg                         <  1:71558/138‑1 (MQ=255)
                                                                                                         aaatcgtctGAGATAAAAAAACCGGCCTCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCg                      <  2:232056/133‑1 (MQ=255)
                                                                                                           aaGCACTGAAATAAAAAACCCGGCCGCGCCGAATCAGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCa                    <  6:207475/139‑1 (MQ=255)
                                                                                                            aGCGCTGACAAAAAACACCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCAt                   <  2:249985/139‑1 (MQ=255)
                                                                                                             gccctGAGCCAAAAACCCCGGCCGCGCCGAATCCGATGCTGAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTAGCTGAGATAAAATGGTTAACGTCGCAtt                  <  1:43438/136‑1 (MQ=255)
                                                                                                                  tagacaAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTa              <  4:382816/137‑1 (MQ=255)
                                                                                                                      taaaaaaaCCGGAAGAGCCGAATCCGATGCTTATGCCTGTTAATAAAACAACAAAAGCTTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGcc         <  7:126558/138‑1 (MQ=255)
                                                                                                                      caaaaaacCCTGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCATTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGcc         <  4:292091/138‑1 (MQ=255)
                                                                                                                      caaaaaaaCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGcc         <  3:66571/138‑1 (MQ=255)
                                                                                                                       aaaaaaaCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGcc         >  7:337051/1‑138 (MQ=255)
                                                                                                                        aaaaaaCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGCCCa       <  3:371849/139‑1 (MQ=255)
                                                                                                                             aCCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGCCCAAAATa  <  8:337069/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                          
CTGATAAAGTTATAACCAAACAAAAATAAAATGCCGATAAATTGAATCTGTAAATACGTCTCAGCAAGCGGTATCATTGACTCCGGCGTTTTTAGAAGACGCAGCAAAAGCTCTGAGAGAAAAAAACCGGCCGCGCCGAATCCGATGCTTAGGCCTGTTAATAAAACAACAAAAGCGTTAATATACGACGCCATTCCTTTTATATCATCTTTTCCTTTTTGCTGAGATAAAATGGTTAACGTCGCATTGTTAAGGCCCAAAATA  >  CP006881/2107402‑2107665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑