Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,136,638	A→G	5.1%	F220S (TTT→TCT)	U712_10675 ←	Xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,136,638	0	A	G	5.1%	249.1 / 2.9	79	F220S (TTT→TCT)	U712_10675	Xanthine permease
Reads supporting (aligned to +/- strand):  ref base A (43/32);  new base G (2/2);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

AATCTATCTCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTAAAACGG  >  CP006881/2136501‑2136775                                                                                                                                          |                                                                                                                                          aaTCTATCTCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTaaaa                                                                                                                                          >  6:108376/1‑139 (MQ=255)  atctatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCCCAAGGCTGACTATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGCCGGCGCTCCGAAGTAAAAt                                                                                                                                         >  6:416366/1‑139 (MQ=255)  atctatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAAt                                                                                                                                         >  6:352290/1‑139 (MQ=255)  atctatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAAt                                                                                                                                         >  2:83342/1‑139 (MQ=255)  atctatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAAt                                                                                                                                         >  7:413674/1‑139 (MQ=255)  atctatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAAt                                                                                                                                         >  2:50152/1‑139 (MQ=255)  atctatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAAt                                                                                                                                         >  4:419703/1‑139 (MQ=255)  atctatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAAt                                                                                                                                         >  6:9888/1‑139 (MQ=255)     tatctCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCTCTCCGAAGTAAAATGGc                                                                                                                                      >  8:257860/1‑139 (MQ=255)        ctctGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTg                                                                                                                                    >  5:195609/1‑138 (MQ=255)            gTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCa                                                                                                                               >  1:97650/1‑139 (MQ=255)            gTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCa                                                                                                                               >  1:243223/1‑139 (MQ=255)             tCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCAt                                                                                                                              >  1:396208/1‑139 (MQ=255)             tCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGCGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCAt                                                                                                                              >  6:429447/1‑139 (MQ=255)              cacaCGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCAtt                                                                                                                             >  7:37593/4‑139 (MQ=255)              cAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCAtt                                                                                                                             >  5:421977/1‑139 (MQ=255)                aaCGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTg                                                                                                                           <  5:9888/139‑1 (MQ=255)                       gTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACt                                                                                                                    >  3:255062/1‑139 (MQ=255)                          tGTAAGGTCCCATAAAGCAAATAAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATGGTAATTATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGc                                                                                                                  <  7:257860/138‑1 (MQ=255)                           gTCAGGTCACCTAACGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGcc                                                                                                                 <  4:250792/138‑1 (MQ=255)                                              aGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACAATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCaaa                                                                                             >  6:13673/1‑139 (MQ=255)                                              aGTAAACACCAGTTGACTCCACAAGGCTGACAAATGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCaaa                                                                                             >  4:437516/1‑139 (MQ=255)                                              aGTAAACACCAGTTCACTCCACAAGGCTGACAATTCCAACGATGGACATCGTAATGATAGGCGCTGCGTCAAAAGACGGCGCTCCGAAGTAAAATGTCAGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCaaa                                                                                             >  3:252012/1‑139 (MQ=255)                                                   acacCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGac                                                                                        >  8:398813/1‑138 (MQ=255)                                                      ccAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACtt                                                                                     <  2:243223/139‑1 (MQ=255)                                                        aGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACtttt                                                                                   >  3:135116/1‑139 (MQ=255)                                                          ttGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTcc                                                                                 >  3:58264/1‑139 (MQ=255)                                                            gACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGGGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTccc                                                                                <  4:276935/138‑1 (MQ=255)                                                               tCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAACCATTATCAAATTGAACTTTTCCCATa                                                                             >  4:341954/1‑138 (MQ=255)                                                               tCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATa                                                                             >  1:313735/1‑138 (MQ=255)                                                               tCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATa                                                                             >  4:275968/1‑138 (MQ=255)                                                                 cacaAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATaaaa                                                                          >  6:189922/1‑139 (MQ=255)                                                                 cacaAGGCTGACAATTGCAACGATGGACATCG‑AATGATAGGCGCTGCGTGAAAAGCCGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATaaaa                                                                          >  7:103201/1‑138 (MQ=255)                                                                   caAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAata                                                                        >  4:384565/1‑139 (MQ=255)                                                                    aaGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCTCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAatat                                                                       >  4:172523/1‑139 (MQ=255)                                                                                 gCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCTATAAAATATGCGATGAAGGTGc                                                          >  6:276774/1‑139 (MQ=255)                                                                                 gCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTg                                                           <  3:437516/138‑1 (MQ=255)                                                                                  cAACGCTGGACATCGTAATGAACGGCGCGGCGTGAAACGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  6:57885/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCTCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  3:172523/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCTATAAAATATGCGATGAAGGTGcc                                                         <  5:276774/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATCTGCGATGAAGGTGcc                                                         <  2:313735/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  7:105551/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  8:413674/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  1:50152/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  1:83342/139‑1 (MQ=255)                                                                                  cAACGATGGACATCGTAATGATAGGCGCTGCGCGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  5:429447/139‑1 (MQ=255)                                                                                       aTGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGcc                                                         <  1:388702/134‑1 (MQ=255)                                                                                       aTGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATc                                                     >  5:339929/1‑138 (MQ=255)                                                                                       aTGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGAGGAAGGTGcc                                                         >  2:388702/1‑134 (MQ=255)                                                                                              tCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATAcc                                             <  4:255062/139‑1 (MQ=255)                                                                                               cGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATAcc                                             >  4:56564/1‑138 (MQ=255)                                                                                                    tGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCa                                        >  2:259398/1‑138 (MQ=255)                                                                                                        aGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATc                                   >  5:326268/1‑139 (MQ=255)                                                                                                         ggCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCcg                                                                                                                >  7:123523/1‑61 (MQ=255)                                                                                                         ggCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCcg                                                                                                                <  8:123523/61‑1 (MQ=255)                                                                                                         ggCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAAt                                    >  4:199109/1‑137 (MQ=255)                                                                                                         ggCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAAt                                    <  3:199109/137‑1 (MQ=255)                                                                                                         ggCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATc                                   >  7:384456/1‑138 (MQ=255)                                                                                                         ggCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATc                                   >  5:92078/1‑138 (MQ=255)                                                                                                           cgcTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCgag                                <  5:352290/139‑1 (MQ=255)                                                                                                              tGCGTGAAAAGACGGCGCTCCGAAGTAAGATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACg                             >  7:200382/1‑139 (MQ=255)                                                                                                              tGCGTGAAAAGACGGCGCTCCGAAGTAAGATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACg                             >  7:206215/1‑139 (MQ=255)                                                                                                                cGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCGCATAAAATATGCGATGAAGGTGCCACTCAAAATACCGATCAAAATCGAGACGGa                           >  5:238763/1‑139 (MQ=255)                                                                                                                cGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGa                           >  7:309176/1‑139 (MQ=255)                                                                                                                  tGAAACGACGGCGCTCCGAAGTAAAATGGCAGAATCATTTGCACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGCTGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACt                         <  2:213049/139‑1 (MQ=255)                                                                                                                          cGGCGCTCCGAAGTAAAATGGCTGAAACATTTTAACAACTGCCGCCTCCGAAACATTATCAAATTGAACTTTTCACATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCTAGCCGGACATGATaaac                 >  2:128298/1‑138 (MQ=255)                                                                                                                               cTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCttt            >  3:408646/1‑139 (MQ=255)                                                                                                                                 ccGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTg          <  4:283344/139‑1 (MQ=255)                                                                                                                                    aaGTTAAATGGCGCACTCCTTAGAACAACTTCCGCGTACGAAACATTATCAAATTGAACTTTACCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGCCGGACTTGATAAAGCCTTTTGTaa       <  4:252012/139‑1 (MQ=255)                                                                                                                                      gTAAGATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  8:206215/139‑1 (MQ=255)                                                                                                                                      gTAAGATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  8:200382/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGTAACATTATCATTTTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  1:128298/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCACATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  8:37593/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  6:92078/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  1:377420/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  6:421977/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  1:259398/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  8:309176/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  1:200954/139‑1 (MQ=255)                                                                                                                                      gTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTaaaa     <  5:13673/139‑1 (MQ=255)                                                                                                                                         aaaTGTCTGAATCAATAGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTAAAAc    <  6:238763/137‑1 (MQ=255)                                                                                                                                          aaTGGCTGAGTCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTAAAACgg  >  6:247811/1‑138 (MQ=255)                                                                                                                                          |                                                                                                                                          AATCTATCTCTGTCAAACGGCGGTTTGTCAGGTCACCTAAAGCAAAGTAAACACCAGTTGACTCCACAAGGCTGACAATTGCAACGATGGACATCGTAATGATAGGCGCTGCGTGAAAAGACGGCGCTCCGAAGTAAAATGGCTGAATCATTTGAACAACTGCCGCGTCCGAAACATTATCAAATTGAACTTTTCCCATAAAATATGCGATGAAGGTGCCAATCAAAATACCGATCAAAATCGAGACGGACTTGATAAAGCCTTTTGTAAAACGG  >  CP006881/2136501‑2136775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑