Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,289,100 G→T 100% P184T (CCG→ACG)  U712_11585 ← putative oxidoreductase yqjQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,289,1000GT100.0% 131.5 / ‑6.6 44P184T (CCG→ACG) U712_11585putative oxidoreductase yqjQ
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (20/21);  total (20/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCCGCTTGAGA  >  CP006881/2288967‑2289235
                                                                                                                                     |                                                                                                                                       
aTCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCg                                                                                                                                    >  6:320628/1‑139 (MQ=255)
     ccGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTg                                                                                                                               <  1:114501/139‑1 (MQ=255)
     cagCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGACTTTATCAGCAAAGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGtt                                                                                                                                >  1:7142/3‑138 (MQ=255)
              cGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACtgttgt                                                                                                                       <  1:208289/138‑1 (MQ=255)
                           gCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCgg                                                                                                         <  1:345463/139‑1 (MQ=255)
                           gCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCGGTATCAGCAATGGAAAAAAAGTCCGTCTGACTCGTGCCCGGGGTGCCTGTTGTCCCCTTCATGCCgg                                                                                                         >  4:282857/1‑139 (MQ=255)
                               tAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGtt                                                                                                       <  2:7142/137‑1 (MQ=255)
                                      aGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGaagg                                                                                               >  8:82807/1‑136 (MQ=255)
                                      aGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGc                                                                                              >  8:274261/1‑139 (MQ=255)
                                                tcatcaGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCa                                                                                    <  5:252962/139‑1 (MQ=255)
                                                tcatcaGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCa                                                                                    <  1:285541/139‑1 (MQ=255)
                                                       gATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGG‑AAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGttt                                                                            >  8:124984/1‑139 (MQ=255)
                                                           caGCATCCAGCGGCCTACATATTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAACTCCGTCTGAATCGTGCCCGGGTTGACTGATGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  3:305916/138‑1 (MQ=255)
                                                           aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  5:320628/139‑1 (MQ=255)
                                                           aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  1:52776/139‑1 (MQ=255)
                                                           aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  7:274261/139‑1 (MQ=255)
                                                           aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  4:176621/139‑1 (MQ=255)
                                                           aaGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGG‑AAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  7:124984/138‑1 (MQ=255)
                                                            aGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         >  7:328977/1‑138 (MQ=255)
                                                            aGCATCCAGCGGCCGACATTATTGGCGTAGTCCCCGCCGTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTGGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         >  6:128360/1‑138 (MQ=255)
                                                                 ccAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAAcc                                                                    >  4:183246/1‑138 (MQ=255)
                                                                  cAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGG‑AAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         >  2:69786/1‑131 (MQ=255)
                                                                  cAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGG‑AAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  1:69786/131‑1 (MQ=255)
                                                                   aGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTaa                                                                 <  8:328977/139‑1 (MQ=255)
                                                                       gCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATCGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGCCTGTTGTCCCATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGCGTAGCCTaccccg                                                             >  2:114527/1‑134 (MQ=255)
                                                                                  tGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGAGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTgg                                                   <  7:31141/138‑1 (MQ=255)
                                                                                    gCGTATGCCCCGCCTATATCAGCAATGGAAAAAAAGGCCGTCTGAATCGCGCCCGGGTGGCCTGTTGTCACATAACTGCCGCTTCCCGAAAGCGCCATCCGCAAAGCGTTTGAGAAACCTAACACGGCATGTTTGGGcg                                                <  7:207354/139‑1 (MQ=255)
                                                                                     cGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGGCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTcgc                                               <  5:167458/139‑1 (MQ=255)
                                                                                       tAGTCCCCGCGTTTATCAGCAAAGGAAAAAAAGTCCGTCTGAATCGTGGCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCCCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTcgcg                                              >  2:198054/1‑138 (MQ=255)
                                                                                         gTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGaa                                           >  2:217476/1‑139 (MQ=255)
                                                                                           ccccGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTGGACTGTTGTCACATAAATGCCGGTGCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATa                                         <  2:37329/139‑1 (MQ=255)
                                                                                                ccTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGCTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGc                                    <  8:338258/139‑1 (MQ=255)
                                                                                                    tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTa                                  <  7:435069/137‑1 (MQ=255)
                                                                                                    tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTa                                  >  8:435069/1‑137 (MQ=255)
                                                                                                    tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGa                                >  6:107027/1‑139 (MQ=255)
                                                                                                    tATCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCAGCAAAGCGTTTGAGTAACCTAACACGGCAAGTTTGGTCGCGGAATACAGGCTAGa                                >  5:35051/1‑139 (MQ=255)
                                                                                                      tCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGa                                                                                                  >  1:92720/1‑71 (MQ=255)
                                                                                                      tCAGCAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGa                                                                                                  <  2:92720/71‑1 (MQ=255)
                                                                                                          cAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         >  4:390509/1‑92 (MQ=255)
                                                                                                          cAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAg                                                                         <  3:390509/92‑1 (MQ=255)
                                                                                                          cAATGGAAAAAAAGTCCGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCgg                          >  2:116380/1‑139 (MQ=255)
                                                                                                                          cGTCTGAATCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTcccc          >  1:375798/1‑139 (MQ=255)
                                                                                                                                 aTCGTGCCCGGGTTGACTGTTGTCACATAAATGCCGGTGCCCGAAAGCTCCAGCCGCACAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCCGCTTgag   <  1:401382/139‑1 (MQ=255)
                                                                                                                                   cGGGCCGGGGTTGTATGTTGTAACATAAATGCCGTTTCCCGAATGCTCCTTCCGAAAAGGTTTTGATTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCCGCTTgaga  <  6:35051/138‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
ATCTCCCGCTTTTTCGTAAAAATTGCAGCTGTAATTTGAGCTGCCACGTCATCAGGATCAAGCATCCAGCGGCCGACATTTTTGGCGTAGTCCCCGCCTTTATCAGCAATGGAAAAAAAGTCCGTCTGAATCGGGCCCGGGTTGACTGTTGTCACATAAATGCCGGTTCCCGAAAGCTCCATCCGCAAAGCGTTTGAGTAACCTAACACGGCATGTTTGGTCGCGGAATACAGGCTAGACTTCGGTGTGGCGATTTTCCCCGCTTGAGA  >  CP006881/2288967‑2289235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑