Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,455,144 C→T 5.5% L13L (CTG→CTA)  U712_12510 ← Hypothetical Protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,455,1440CT 5.5% 233.0 / 3.0 72L13L (CTG→CTA) U712_12510Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base C (29/39);  new base T (2/2);  total (31/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

TCTCTTTCATCATCCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGAAATGATGCTGTAGATTCAGCTGT  >  CP006881/2455009‑2455281
                                                                                                                                       |                                                                                                                                         
tctctTTCATCATCCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCCGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACcag                                                                                                                                         >  3:195530/1‑138 (MQ=255)
   ctTTCATCATCCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACcagaa                                                                                                                                       >  1:40537/1‑137 (MQ=255)
    tttCATCATCCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACcagaa                                                                                                                                       >  6:203650/1‑136 (MQ=255)
         tcatcCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAAc                                                                                                                               >  4:54075/1‑139 (MQ=255)
          catcCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACg                                                                                                                              <  3:54075/139‑1 (MQ=255)
           atcCCCTTCTATCCTAAATTGAGATTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATAATAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACg                                                                                                                              <  2:440318/138‑1 (MQ=255)
            tcCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACTAGAACAGAAAACGt                                                                                                                             >  3:187854/1‑138 (MQ=255)
            tcCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACTAGAACAGAAAACGt                                                                                                                             >  3:180979/1‑138 (MQ=255)
            tcCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGt                                                                                                                             >  5:308858/1‑138 (MQ=255)
                cTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCt                                                                                                                        <  6:308858/139‑1 (MQ=255)
                  tCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCt                                                                                                                        >  6:168689/1‑137 (MQ=255)
                           aaTTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTc                                                                                                                 >  3:343511/1‑135 (MQ=255)
                           aaTTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTc                                                                                                                 >  3:190694/1‑135 (MQ=255)
                                aGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGAttttt                                                                                                        <  4:122881/139‑1 (MQ=255)
                                aGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGAttttt                                                                                                        >  6:87737/1‑139 (MQ=255)
                                aGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGAttttt                                                                                                        >  7:76336/1‑139 (MQ=255)
                                   ttGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCa                                                                                                      >  1:76833/1‑138 (MQ=255)
                                        aaaTAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACaa                                                                                                <  2:40537/139‑1 (MQ=255)
                                        aaaTAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACaa                                                                                                <  2:288039/139‑1 (MQ=255)
                                        aaaTAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACaa                                                                                                <  6:49762/139‑1 (MQ=255)
                                         aaTAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAAt                                                                                               <  1:114430/139‑1 (MQ=255)
                                          aTAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAAtt                                                                                              <  8:76336/139‑1 (MQ=255)
                                             gTAAAGAGGAAGCCTGACTGCTGCATCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCa                                                                                           <  1:13499/139‑1 (MQ=255)
                                              tAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCtt                                                                                                                       >  4:279555/1‑110 (MQ=255)
                                              tAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCtt                                                                                                                       <  3:279555/110‑1 (MQ=255)
                                                aaGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCAtt                                                                                         <  2:3549/138‑1 (MQ=255)
                                                aaGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCAtt                                                                                         <  3:424337/138‑1 (MQ=255)
                                                aaGACGAAGCCTGACTGCTGCTTCCCCATTAATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCAtt                                                                                         <  5:47500/138‑1 (MQ=255)
                                                     gAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACt                                                                                    >  1:398143/1‑138 (MQ=255)
                                                     gAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACt                                                                                    >  2:315487/1‑138 (MQ=255)
                                                      aaGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACtt                                                                                   >  1:47563/1‑138 (MQ=255)
                                                       aGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTaa                                                                                 <  2:47563/139‑1 (MQ=255)
                                                        gCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGACAACGTTTCCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAag                                                                                <  4:437104/139‑1 (MQ=255)
                                                        gCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAag                                                                                <  2:398143/139‑1 (MQ=255)
                                                        gCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAag                                                                                <  8:367412/139‑1 (MQ=255)
                                                         ccTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACTAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAagt                                                                               <  4:187854/139‑1 (MQ=255)
                                                         ccTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACTAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAagt                                                                               <  4:180979/139‑1 (MQ=255)
                                                         ccTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAagt                                                                               <  1:70242/139‑1 (MQ=255)
                                                               tgctgcTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAagtcatt                                                                           >  7:249145/1‑135 (MQ=255)
                                                                 ctgctTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCagg                                                                       <  1:155699/139‑1 (MQ=255)
                                                                 ctgctTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCagg                                                                       <  5:323663/139‑1 (MQ=255)
                                                                 ctgctTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCagg                                                                       <  1:83687/139‑1 (MQ=255)
                                                                 ctgctTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCagg                                                                       <  3:13190/139‑1 (MQ=255)
                                                                 ctgctTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCagg                                                                       <  3:182254/139‑1 (MQ=255)
                                                                 ctgctTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCagg                                                                       <  1:156050/139‑1 (MQ=255)
                                                                      tCCCCATTTATATTACACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGa                                                                  <  4:57923/139‑1 (MQ=255)
                                                                         ccATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATaa                                                               <  5:168689/139‑1 (MQ=255)
                                                                           aTTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCGTGTAAAAAGGCGATAATCACCAGAACAGCAACCGTTACCTTAGCTTCTAGATTTTGCAAAACAATTCGATTATACTTAAGTCAGTCAGGAGGGATAAAg                                                             >  8:110198/1‑139 (MQ=255)
                                                                            tttATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGAttttt                                                                                                        <  7:285091/95‑1 (MQ=255)
                                                                            tttATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGAttttt                                                                                                        >  8:285091/1‑95 (MQ=255)
                                                                                     aaCGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAagt                                                                               >  4:317721/1‑111 (MQ=255)
                                                                                     aaCGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAagt                                                                               <  3:317721/111‑1 (MQ=255)
                                                                                     aaCGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGAtt                                                   >  7:86339/1‑139 (MQ=255)
                                                                                          cTGTTCGCAAGCCCCCGGGCCCGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGGTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGa                                               >  5:206852/1‑138 (MQ=255)
                                                                                          cTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGa                                               >  4:281075/1‑138 (MQ=255)
                                                                                          cTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGa                                               >  2:350455/1‑138 (MQ=255)
                                                                                          cTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGa                                               >  3:7054/1‑138 (MQ=255)
                                                                                               cGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGaaaaaa                                          <  8:182794/138‑1 (MQ=255)
                                                                                                    gCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACaa                                                                                                >  2:48997/1‑79 (MQ=255)
                                                                                                    gCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACaa                                                                                                <  1:48997/79‑1 (MQ=255)
                                                                                                        ccGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAg                                <  1:418611/139‑1 (MQ=255)
                                                                                                         cGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAg                                >  2:319636/1‑138 (MQ=255)
                                                                                                            gACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCaaa                            <  1:319636/139‑1 (MQ=255)
                                                                                                                gCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACaa                                                                                                <  1:132951/67‑1 (MQ=255)
                                                                                                                gCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACaa                                                                                                >  2:132951/1‑67 (MQ=255)
                                                                                                                gCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAag                                                                                >  5:102444/1‑83 (MQ=255)
                                                                                                                gCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAag                                                                                <  6:102444/83‑1 (MQ=255)
                                                                                                                gCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGa                         >  2:209268/1‑138 (MQ=255)
                                                                                                                gCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGa                         >  2:288973/1‑138 (MQ=255)
                                                                                                                gCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGa                         >  1:439432/1‑138 (MQ=255)
                                                                                                                 cTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAAc                                                                                                  <  3:346309/64‑1 (MQ=255)
                                                                                                                 cTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAAc                                                                                                  >  4:346309/1‑64 (MQ=255)
                                                                                                                 cTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGaaa                       <  3:108281/139‑1 (MQ=255)
                                                                                                                 cTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGaaa                       <  4:7054/139‑1 (MQ=255)
                                                                                                                    gTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGAAatg                     <  2:76833/138‑1 (MQ=255)
                                                                                                                                      ccAGAACAGAAAGCGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGAAATGATGCTGTAGATTCAGCTGt  >  3:375205/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                         
TCTCTTTCATCATCCCCTTCTATCCTAAATTGAGTTTGCAAAATAGTAAAGAGGAAGCCTGACTGCTGCTTCCCCATTTATATTAAACGCCTGTTCGCAAGCCCCCGGGACAGCTTGTAAAAAGGCGATAATCACCAGAACAGAAAACGTTACCTTAGCTTCTAGATTTTTCAAAACAATTCCATTATACTTAAGTCAGTCAGGAGGGATAAAGTTGAATGATTTTGAAAAAAACGTTCAAAGCAAACGAAATGATGCTGTAGATTCAGCTGT  >  CP006881/2455009‑2455281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑