Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,643,024 A→C 17.0% F204V (TTT→GTT)  U712_13595 ← Single‑stranded‑DNA‑specific exonuclease recJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,643,0240AC17.0% 93.9 / 4.4 55F204V (TTT→GTT) U712_13595Single‑stranded‑DNA‑specific exonuclease recJ
Reads supporting (aligned to +/- strand):  ref base A (33/12);  new base C (2/8);  total (35/20)
Fisher's exact test for biased strand distribution p-value = 2.77e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGCCTTTCAAGTCCTAGTGTTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTAATGATGACATCCAGTCCGAG  >  CP006881/2642888‑2643156
                                                                                                                                         |                                                                                                                                    
cGAAGCCTTTCAAGTCCTAGTGTTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAAcg                                                                                                                                    >  7:268587/1‑139 (MQ=255)
 caaGCCTTTCAAGTCCTAGTGTTGCTATTAATCTGTTTTCGTCATGCCGCGGGACTAGAGCGCCAA‑TGGGGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAACGCGTGGGCAAGCTTAAAcgc                                                                                                                                   <  1:273420/138‑1 (MQ=255)
     ccTTTCAAGTCCTAGTGTTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACt                                                                                                                               >  8:264057/1‑139 (MQ=255)
                cTAGTGTTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGCTCGCCGCCAGGTCAAGCAGGTCATCAGGCAGTTCACCCAATAACGCGTGGGCCAGCTGAACCGCGACTCCCCCCCCGGc                                                                                                                    >  8:403254/1‑139 (MQ=255)
                cTAGTGTTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCACCCCCgg                                                                                                                     >  7:290039/1‑138 (MQ=255)
                  aGTGTTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCACCCCCGGCac                                                                                                                  >  6:209144/1‑138 (MQ=255)
                    tgtTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAg                                                                                                                 <  8:268587/138‑1 (MQ=255)
                    tgtTGATTTTAATTTGTTTTCTTCTTCCAGCGGTACTTTATCGGAAT‑TGGTGCTGTTTGCCGCCTGTTAATGATTTTCATCTGGAAGTTCACCAATTAATGGGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAg                                                                                                                 <  6:400091/138‑1 (MQ=255)
                       tGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAACGCGTGGGCAAGCTTAACCGCGACTCCACCCCCGGCAAGctct                                                                                                             >  1:68658/1‑139 (MQ=255)
                        gCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCtt                                                                                                            >  4:386833/1‑139 (MQ=255)
                        gCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCtt                                                                                                            >  3:334539/1‑139 (MQ=255)
                        gCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGAGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCtt                                                                                                            >  4:162321/1‑139 (MQ=255)
                         cTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCTTGGGCAAGCTTAAACGCTACTCCAACCCAGGCAAGCTCtt                                                                                                            >  8:351195/1‑138 (MQ=255)
                         cTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCtt                                                                                                            >  1:362611/1‑138 (MQ=255)
                         cTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCAGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCtt                                                                                                            >  5:400091/1‑138 (MQ=255)
                            ttAATATTTTTTCGTCATGCCGCGTGACTAGATCGGAAC‑TGGTGCCTATAGCGCCGATGTCAATCATGTGATATGGATTTTCACCAAATAACGCGTGGTCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTaaa                                                                                                        <  8:257042/139‑1 (MQ=255)
                                    ttttCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAg                                                                                                >  7:257042/1‑139 (MQ=255)
                                    ttttCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAg                                                                                                >  3:208679/1‑139 (MQ=255)
                                    ttttCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAg                                                                                                >  1:113215/1‑139 (MQ=255)
                                              gCAGGGGCACTTGAT‑GGCAACTGGTGCCGATAGCCGACTGTTCAAGCTTTTAATCAGTAAGTTCACCCAATAAAGCGTGGGCAAGCTAAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAgg                                                                                      <  8:7083/139‑1 (MQ=255)
                                              gCAGCGGCAATAGCGCGGCAT‑TGGGCCCGATAGCCGCCAGGTCAAGCAGTTCATCAGTCAGAACACCCAATAACGCGTGGGCATGCTTAACCGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAgg                                                                                      <  7:351195/139‑1 (MQ=255)
                                                  gggcaCTACATCGGAAT‑TGGTGCCGATCGCCGCCAGGTCAAGCAGTTCATCAGTAAGTCCACCCAATAACGGGTGGCCAAGCTAAACCGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGgctgc                                                                                  <  4:378260/135‑1 (MQ=255)
                                                          gATCGGCAA‑TGGTGCCGATAGCCGCCCGGTCAAGCAGTTCCTCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTACACGCGACTCCACCCCCGGCAAGCGCTGTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTg                                                                          >  3:208089/1‑139 (MQ=255)
                                                               gCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTGACCCAATAAAGCGTGGGCAAGCTTAAACGCGCCTCCAGCCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCCGGCTGCTTCGGGTGGACGa                                                                     >  6:392620/1‑139 (MQ=255)
                                                               gCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCATTAAAGCGTGGGCAAGCTTAAACGCGACTCCACCCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCGTCGGGTGGACGa                                                                     >  3:422560/1‑139 (MQ=255)
                                                               gCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGtctgtct                                                                     >  3:38775/1‑133 (MQ=255)
                                                               gCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGa                                                                     >  5:208552/1‑139 (MQ=255)
                                                                        gCCTATAGCCGCCAGGTAAAGCTGTTCATCAGTAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACgg                                                             <  8:290039/139‑1 (MQ=255)
                                                                         ccGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGa                                                            >  3:127897/1‑139 (MQ=255)
                                                                         ccGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGAGACTCCCACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGCCGATCGCACGGa                                                            >  6:97573/1‑139 (MQ=255)
                                                                                            gCAGTTCATCAGGAAGTACACCCAATAACGCGTGGCCAAGCTTAACCGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTcc                                         <  3:409868/139‑1 (MQ=255)
                                                                                            ccAGATCATCAGGACGTTCACCCAATAACGCGGGGGCAAGCTTAACCGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTcc                                         <  3:321285/138‑1 (MQ=255)
                                                                                             ctgTTCATCAGGAAGTTCACCCAATAAAGCGGGGTCAAGCTTAACCGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTccc                                        <  2:362611/137‑1 (MQ=255)
                                                                                                ttCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGt                                     >  1:317730/1‑139 (MQ=255)
                                                                                                  catcaGGAAGTTCACCCAATAAAGCGGGGGCAAGCTTAAACGCGACTCCCACCCCGGCACGCTCTTTAAACGGGTAAGTGCCGCCCGGCTGCGTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGtt                                    >  6:328459/1‑138 (MQ=255)
                                                                                                     caGGAAGTTCAACCAATAAAGATTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAATCTCTTTAAACTGGTAAGTGCATCCAGGCTGCTTCGGGTGGACGATCTCACGGACATCCTGAATCTCCGGTCCCGGTTCat                                 >  4:243429/1‑138 (MQ=255)
                                                                                                          tgtacACCAAATAAAGGGTGCGCACGTTAAACCGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCATGGACATCCGGAAGCTCCGGTCCCGGTTCATgatgat                           <  3:320854/135‑1 (MQ=255)
                                                                                                          tgTTCACCAAATAATGGGTGGGCATGTTTAACCGCGACTCCAACCCCGGCTAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATgatgat                           <  3:162321/138‑1 (MQ=255)
                                                                                                          agatCACCAAATAATGCGTGGTCAATATAAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATgatgat                           <  3:386833/136‑1 (MQ=255)
                                                                                                          aGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATgatgat                           >  4:430115/1‑139 (MQ=255)
                                                                                                          aGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATgatgat                           >  7:331727/1‑139 (MQ=255)
                                                                                                              cACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCgg                        >  7:250281/1‑138 (MQ=255)
                                                                                                                cccAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTaa                     <  8:331727/139‑1 (MQ=255)
                                                                                                                ccaaaTAACGGGTGGGCAATATAAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTaa                     <  2:317730/136‑1 (MQ=255)
                                                                                                                  cAATAACGCGTGGCCAAGCTTAACCGCGACTCCAACCCCGGCAAGCTCTTTATAGGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGGTCATGATGATCGGTAatg                   <  4:422560/139‑1 (MQ=255)
                                                                                                                     tAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTAatgat                 >  3:418076/1‑138 (MQ=255)
                                                                                                                          cGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTAATGATGACATc           >  6:339644/1‑139 (MQ=255)
                                                                                                                           gTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGCTGCTCGGTAAGGATGCCATcc          >  6:32130/1‑139 (MQ=255)
                                                                                                                             gggCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTAATGATGACATCCAg        >  2:308030/1‑139 (MQ=255)
                                                                                                                              ggCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCTGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCAtgatga                            <  7:394727/118‑1 (MQ=255)
                                                                                                                              ggCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGATGCTCCGGTCCCGGTTCATGTTGATCGGTAATGATGACATCCAGt       >  7:26099/1‑139 (MQ=255)
                                                                                                                              ggCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCAtgatga                            >  8:394727/1‑118 (MQ=255)
                                                                                                                                   gattAAACGCGACTCAAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCGGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTAATGATGACATCCAGTCCGAg  <  6:333522/137‑1 (MQ=255)
                                                                                                                                    cTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGt                                                                                                   <  6:443380/41‑1 (MQ=255)
                                                                                                                                    cTTAAACGCGACTCAAACCCCGGCAAGCTCTTTAAACGGGt                                                                                                   >  5:443380/1‑41 (MQ=255)
                                                                                                                                         |                                                                                                                                    
CGAAGCCTTTCAAGTCCTAGTGTTGCTATTAATCTGTTTTCGTCATGCAGCGGGACTAGATCGGCAA‑TGGTGCCGATAGCCGCCAGGTCAAGCAGTTCATCAGGAAGTTCACCCAATAAAGCGTGGGCAAGCTTAAACGCGACTCCAACCCCGGCAAGCTCTTTAAACGGGTAAGTGCAGCCAGGCTGCTTCGGGTGGACGATCGCACGGACATCCGGAAGCTCCGGTCCCGGTTCATGATGATCGGTAATGATGACATCCAGTCCGAG  >  CP006881/2642888‑2643156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑