| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 3,410,425 | T→C | 6.6% | I275M (ATA→ATG) | U712_17515 ← | putative membrane protein yvoD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,410,425 | 0 | T | C | 6.6% | 183.2 / 2.1 | 62 | I275M (ATA→ATG) | U712_17515 | putative membrane protein yvoD |
| Reads supporting (aligned to +/- strand): ref base T (25/32); new base C (2/2); total (27/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATCGTCGTTACTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACACCCGCTCCG > CP006881/3410287‑3410557 | gAATCGTCGTTACTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGt < 5:419737/139‑1 (MQ=255)caaTCGTCGTTACTTGTTTGGCACTCATATTGCTTCCTTTCCAACGTCTGCGGGTTTCCATTTTTACCCCGTGGGCGCAATCCCCATTGTCAGCACAACTGCTGTGGTTACACGAAAAAAATGACGCGGCCAG‑ACCGGt < 4:319985/138‑1 (MQ=255) tttACTTTTTTGTCACTCATATTGCTTCCTTACCAACCGGTGTGGGTTTCCTTTTTTTCCACGTGTGCGAAATAGCCTTTGTCAGCAGAACTGCTGTGGTGACACTAATCAAAACACGCGGCCAG‑ACCGGTATGCCAAg < 4:256220/138‑1 (MQ=255) gtTACTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAg < 7:305068/139‑1 (MQ=255) gtTACTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCGGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAg < 8:266291/139‑1 (MQ=255) gtTACTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCCCAATAGCCATTGTCACCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAg < 1:264267/139‑1 (MQ=255) gtTACTTGTTTGTCACTCATATTGCTTCCTTACCAACCGCTTAGGGTTCCCATTTTTTCCACGTGGGCGCAATCCCCATTGGCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAg < 8:64323/139‑1 (MQ=255) ttACTTGTTTGTCACTCATCTTGCTTCCTTCCCAACGGCTGAGGGTTTCCATTTTTTCCACGTGGGCGCACACGCCATTGTCCGCAGAACTGCTGGGGGGACACGACTCAAACTACGCGGCCAG‑ACCGGTATGCCAAGc < 8:232963/139‑1 (MQ=255) ttACTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCTTTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGGGACACGAATCAAAAGACGCGGCCAG‑ACCGGTATGCCAAGc < 3:310641/139‑1 (MQ=255) tACTGGTTGGTCACTCATATTGCTAGCTTTCCACCCGCTCAGGGTTTCCATTTTTCCCCCGTGACCCAAATAGCCATGCTCCGCAGTACTGCGGTGGTCACACTAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGc < 8:225989/138‑1 (MQ=255) aCTTGTTTGTCACTCATATTGCTTCCTTTCCAACCTCTGCGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCg < 4:321144/138‑1 (MQ=255) aCTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGGGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCgg < 7:65511/139‑1 (MQ=255) ttttgtCACTCATTTTTCTTCATTTCCACCCGCTGTGGGTTTCCATTTTTTCCCCGTGTGCGCAATAGCCATTGTCAGCAGACCGGTGGTGGTGACACGAATCAAACCACGCGGCCAG‑ACCGGTATGCCAAGCGGAAt < 6:113033/137‑1 (MQ=255) tttgtCACACATAATGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCAACGTGTGCGCAATAGCCATTGTCCGCAGAACTGCTCTGCTGACACGAAACAAAAGAAGCGGCCAG‑ACCTGTATGCCAAGCTGAATa > 4:193706/1‑138 (MQ=255) tgtCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATaaa < 7:57294/138‑1 (MQ=255) cACTCATATTGCTTCTTTTCCACCCGCTGTGGGTTTCCATTTTTTCCAGGTGGGCGCAATAGCCATTGTCAGCATAACTGCGGTGGTGACACGAATCAAAACAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACa < 4:138732/139‑1 (MQ=255) atatTGCTTCCTTTCCAACCGCTGCGGGTTTCCATTTTTTCCACGGGTGCGCAATAGCCATTGCCAGCAGACCTGCTGGGGGGACACGAATCAAAACACGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAgt < 3:263638/137‑1 (MQ=255) atatTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAg < 8:27113/121‑1 (MQ=255) atatTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAg > 7:27113/1‑121 (MQ=255) tattcattCCTTACCATCCGGTGAGGGTTTCAATTTTTTCCAGGTGTGCGAAATAGCAATTGTCACCATAAGTGGTGTGGTGACACGAAACAAATTACGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTa < 6:214204/133‑1 (MQ=255) tatTGCTTCCTTTCCACCCGCTGCGGGTTTCCATTTTTTCCACGTGGGCGCAATAGCAATTGTCAGCAGAACTGCGGGGGGGACACGAATCAAACGACGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAACACAAGTGTa < 7:131100/139‑1 (MQ=255) tatGGCTCCCTTTCCAACCGCGGAGGGTTTCCTTTTTTCCCACGTGTGCGCAATAGCAATTGTCCCCAGACCTGCGGGGGGCACACCAATAAAAACACGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTa < 4:359290/139‑1 (MQ=255) tttGCTTCTTTTCAACCGGCTGTGGTTTTCAATTTTTACCAGGGGGGCGAAATCGCCATTGTCAGAAGACCGGGTGGGTACACCCGATACAAAACAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACATGTGTa < 6:109178/137‑1 (MQ=255) tttGCTTCCTTGCCAACCGCTGCGGGTTTCAATTTTTACCAGGTGTGCGAAATAGCCTTTGTCCGCAAAACTGGTGTGGTGACACTAAAAAAATCACGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTa < 3:264587/137‑1 (MQ=255) tGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑t > 5:92200/1‑139 (MQ=255) gCTTCCTTTCCAACCGCAGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCACAACTGCTGTGGTGACACGAATCAAAAGAAGCTGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑tt > 5:109178/1‑139 (MQ=255) cTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTc > 6:270161/1‑139 (MQ=255) cTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGCATGCCAAGCGGAATAAAGACAAGTGTATC‑TTc > 4:199863/1‑139 (MQ=255) cTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGCATGCCAAGCGGAATAAAGACAAGTGTATC‑TTc > 4:192933/1‑139 (MQ=255) tCCTTTCCAACCGCTGAGGGTTTCCATTTTTACCACGTGAGCGCAATAGCCATTGTCAGCAGCACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCa > 3:369165/1‑139 (MQ=255) tCCTTTCCAACCGCTGAGGGTTTCATTTTTTACCCGGTGTCCGC‑ATTGCCCTTTTAAGCCGGACTTCTGTTGTGCCCCGGATGAAAAGAAGCGGCCCGCACCGTTTTCCCAA‑CGGAATAAAGGCAATTGGATCGTTCCa > 2:404894/1‑139 (MQ=255) aaCCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACcgcg > 3:151982/1‑139 (MQ=255) gAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTggcagg > 1:434863/1‑139 (MQ=255) cATTTTTTCCCCGTGGGCCCAATAGCCTTTGTCAGCACAACTGGTGTGGTCACACTAAAAAAACCACGCGGCCAG‑ACCGGTATGCCAAGCGTAAAAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGaa < 6:361176/139‑1 (MQ=255) ttttCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGCCAAGTGTATC‑TTCCACCACCCCGTGGCAGGCAACCAGAAata > 6:271093/1‑138 (MQ=255) ttCCCCGTGTGCGAAAATCCCTTTGCCAGCTAATCGGTTGTGGTCACACTAAAAAAAACACGCGGCCAG‑ACCGGTATGCCAAGCGTAATAAAGACATGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAAtata < 6:92200/138‑1 (MQ=255) atttGTCAGCAGACCTGCTGGGGTCACACTAATCAAACGACGCGGCCAG‑ACCGGTATGCCACGCGTAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGc < 8:297068/137‑1 (MQ=255) gTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTccctgtctc > 7:345487/1‑133 (MQ=255) agcagAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCa > 6:237912/1‑139 (MQ=255) cagAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGAc < 6:385045/66‑1 (MQ=255) cagAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGAc > 5:385045/1‑66 (MQ=255) cagAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACg > 3:17292/1‑139 (MQ=255) cagAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGACAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGAAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACg > 8:227148/1‑139 (MQ=255) tgctgTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACaa < 7:61505/62‑1 (MQ=255) tgctgTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACaa > 8:61505/1‑62 (MQ=255) tgctgGGGCCACACAAAAAAAACGACGCGGCCAG‑CCCGGTAGGCCAAGCGGAATAAACACAAGTGTATC‑ATCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCa < 7:277325/139‑1 (MQ=255) gtgACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGa > 1:145594/1‑139 (MQ=255) gtgACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGa > 5:262192/1‑139 (MQ=255) gtgACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGa > 4:68610/1‑139 (MQ=255) acacGAATCAAAAGCAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCTACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAg > 4:275823/1‑139 (MQ=255) acacGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAg > 8:132145/1‑139 (MQ=255) aaacacgCGGCCAC‑CCGGTTATGCCAAGCGCAATAACGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCAt < 6:262192/133‑1 (MQ=255) gCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTa < 7:36760/41‑1 (MQ=255) gCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTa > 8:36760/1‑41 (MQ=255) gCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGa < 8:355891/120‑1 (MQ=255) gCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGa > 7:355891/1‑120 (MQ=255) gCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACAcc > 7:110184/1‑139 (MQ=255) cGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACAccc > 4:412/1‑139 (MQ=255) ggCCAG‑CCCGGAAGGCCAACCGGAAAACACCCAAGGGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACACCCg < 5:204884/139‑1 (MQ=255) ggCCAG‑ACGGTTATGCCATGCGTAAAAAAGACCTGGGTATC‑CTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACACCCg < 6:57805/139‑1 (MQ=255) ggCCAG‑ACGGGTATGCCATGCTGAATAACGACAATTGTATC‑TACCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTGATCCTCGACAGCTTTGATCATGACACCCg < 6:86460/139‑1 (MQ=255) ggCCAG‑ACCGGCATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACACCCg < 3:199863/139‑1 (MQ=255) ggCCAG‑ACCGGCATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACACCCg < 3:192933/139‑1 (MQ=255) caCCGGTACGCCAAGCGTAATAACCACAAGTGTTTC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACACCCGCTCCg < 4:151982/138‑1 (MQ=255) | GAATCGTCGTTACTTGTTTGTCACTCATATTGCTTCCTTTCCAACCGCTGAGGGTTTCCATTTTTTCCACGTGTGCGCAATAGCCATTGTCAGCAGAACTGCTGTGGTGACACGAATCAAAAGAAGCGGCCAG‑ACCGGTATGCCAAGCGGAATAAAGACAAGTGTATC‑TTCCACGACCGCGTGGCAGGCAACGAGAAATATAAATGCCAATGTCATGTCCCGCTTGCTCACGCCGTCATCCTCGACAGCTTTGATCATGACACCCGCTCCG > CP006881/3410287‑3410557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |