Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,725,239 2 bp→AA 100% intergenic (‑71/‑457) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,2390GA100.0% 209.8 / NA 61intergenic (‑71/‑458)U712_19205/U712_19210Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/21);  total (40/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*CP0068813,725,2400GA100.0% 177.8 / ‑6.6 61intergenic (‑72/‑457)U712_19205/U712_19210Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (40/20);  minor base C (0/1);  total (40/21)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTT  >  CP006881/3725109‑3725369
                                                                                                                                       ||                                                                                                                                 
cGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTTGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCCCAAGaaaa                                                                                                                              >  5:122742/1‑138 (MQ=255)
     cccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTATAAGCCGCCGCCCGCAACAAAAGACGGc                                                                                                                        >  2:80637/1‑139 (MQ=255)
          ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt                                                                                                                   >  4:378708/1‑139 (MQ=255)
          ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCAGCAAGAAAAGACGGCCGaat                                                                                                                   >  8:429851/1‑137 (MQ=255)
          ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CGTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt                                                                                                                   >  1:29366/1‑139 (MQ=255)
          ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAGC‑CGTTT‑T‑A‑TCCGCCCGGTTTCTCAGCCGCACCCCCCAAGAAAAGAAGGCCgcgg                                                                                                                   >  1:160090/1‑136 (MQ=255)
               caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAGC‑C‑‑TT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttttc                                                                                                            >  3:100804/1‑139 (MQ=255)
               caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttt                                                                                                              >  8:126288/1‑139 (MQ=255)
                aaaaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttctttt                                                                                                             >  4:382248/1‑139 (MQ=255)
                    aGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTATTTTTGGCAGGCAGC‑CTTTT‑T‑A‑ACAGCCCGTTTTCTCATCCCCACCCCGCAACAAAAGACCGCCGATTTATTTTCACg                                                                                                         >  7:160644/1‑139 (MQ=255)
                                cccGAAAAGAAACAAGCAACCGTTTCAAAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGc                                                                                             <  1:408660/139‑1 (MQ=255)
                                agcttAAAGAAACAAGCAATGGTTTCATAGAAAAACATCCCAGCGATTATTTTTGGCAGGCAGC‑T‑TTT‑T‑ATGCCCCCCTTTTTCCCCGCCGCCGCCCGCACGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGc                                                                                             <  8:123947/135‑1 (MQ=255)
                                    tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCCGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGGCGAGTTCTTTTGTCGGAAACTAGGGGctc                                                                                           >  1:279595/1‑137 (MQ=255)
                                    tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                                                           >  7:136224/1‑137 (MQ=255)
                                    tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCggggc                                                                                           >  7:397391/1‑134 (MQ=255)
                                    tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                                                           >  8:242218/1‑137 (MQ=255)
                                       acaTACAAGCAAACTTTTCATACAAAAACATCCCTCCGCTTCTTTTTGGCCGGCTGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCCGCCGCCGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                                                      <  7:429851/137‑1 (MQ=255)
                                       acaTACAACCAACCTTTGCATAAAAAAACACCCCACCGATTATTTTGGCCCGGCTCC‑A‑TTT‑TAA‑GCAGCCCGTTTCCTCCCCCGCACCCCCAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                                                      <  5:140466/137‑1 (MQ=255)
                                       aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                                                      <  7:126288/139‑1 (MQ=255)
                                       aGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                                                      <  1:80637/139‑1 (MQ=255)
                                         aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCGCTAGCaa                                                                                     >  1:49152/1‑138 (MQ=255)
                                          tACAAGCAAACGTTTCATAGAAAAAAATCCCTCCCCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GAAGCCCGTTTTCTCAGCAGCAGCCCGCAAGAAAAGACGGCCGATTTCTTATCTAGCAAACAATCGGCTCTAGCaac                                                                                    >  7:22241/1‑137 (MQ=255)
                                           aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                                                  >  3:124657/1‑139 (MQ=255)
                                           aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                                                  >  7:345503/1‑139 (MQ=255)
                                           aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                                                  >  3:299563/1‑139 (MQ=255)
                                           aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                                                  >  3:118354/1‑139 (MQ=255)
                                           aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCCGCTCAAGCaaaa                                                                                   >  8:282559/1‑138 (MQ=255)
                                           aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTAATTTATAGCAAACAAGCGGCTCTAGCaaaa                                                                                   >  8:175919/1‑138 (MQ=255)
                                           aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCGTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                                                  >  6:120380/1‑139 (MQ=255)
                                             aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTGTTTTTTGCAGGCATCGT‑TTT‑T‑A‑GCAGCCCGTTTTATAAGCCGCAGCCCGCAAGAAAAGACGGACGATTTCTTGTGTGTCAAACATGCGGTTCGAACaaaaat                                                                                 >  6:353776/1‑136 (MQ=255)
                                             aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCTCAAGCCAAACt                                                                                 >  4:103100/1‑138 (MQ=255)
                                             aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTGTAGCCAACTATCGGCGCTAGCAAAAca                                                                                 >  1:230933/1‑137 (MQ=255)
                                             aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCCCAGCCCGCAAGAAAATACGGCCGAGTTCTTTTCTCGCCAACTAGCGGCTCTAGCCAAAcct                                                                                >  7:123947/1‑137 (MQ=255)
                                               gCAACCGTTTCATACAAAAAAAGCCCCCCGCTTCTTTTTGGCCGCCACC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCCCAGCCCCAAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                                                              <  3:398258/139‑1 (MQ=255)
                                               gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑C‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                                                              <  1:1625/139‑1 (MQ=255)
                                               gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                                                              <  8:320780/139‑1 (MQ=255)
                                                 aaaCTTTTCATATAAAAACATCCCTCCGCTTCTTTTTGCCAGGCACCAT‑TTT‑T‑C‑CCAGCCCTTTTTCACCGCCGCAGCCCGAAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                                                            <  8:345503/139‑1 (MQ=255)
                                                  aaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCGAGACAAACTCATGa                                                                            >  6:216563/1‑138 (MQ=255)
                                                   ccttttCATCCAAAAACATCCCGCCGCTTCTTTTTGGCAGGACGC‑C‑TTT‑T‑ACGCACCCCGTTTTCGCAGCCCCACCCCCAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                                                          <  7:197823/136‑1 (MQ=255)
                                                   aCGTTTCATATAAAAAGATCCCGCCGCTGCTTTTTGGCAGGCAGCGT‑TTT‑T‑A‑GCCGCCCGTTTTCTCAGCCCCAGCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                                                          <  1:266343/139‑1 (MQ=255)
                                                   aCGTTTCATATAAAAACATCCCTCCGTTTCTTTTTGGCAGGCAGCTT‑TTT‑T‑T‑CCCGCCGGTTTTCTCAGCCGCAGCGCGAAAAAAATGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                                                          <  7:282559/139‑1 (MQ=255)
                                                   aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                                                          <  3:202184/139‑1 (MQ=255)
                                                   aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                                                          >  3:109979/1‑139 (MQ=255)
                                                   aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTGTTTTCTCGCCAACTAGCGGCTCTAGCAAAACTCATGAct                                                                          >  2:90411/1‑139 (MQ=255)
                                                    cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                                                          >  5:347465/1‑138 (MQ=255)
                                                                 aacaacCCTCCGGTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCTTTTTCGCCGCCCCCCCCCCAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGt                                                            <  8:136224/139‑1 (MQ=255)
                                                                           cGCTTCTTTTTGGCAGGCAGC‑CTTTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGaa                                                  >  5:398337/1‑139 (MQ=255)
                                                                                cttTTTGGCAGGCCCC‑C‑TTTAT‑A‑CCACCCCGTTTACTAACCCGCCCCCCGCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt                                              <  1:23235/138‑1 (MQ=255)
                                                                                    ttgcctggccgC‑C‑TTT‑T‑ACGCCGCCGGTTTTCGCCGCCCCACCCCCCAACAAACCACGGCCGATTTCTTTTCTTGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGt                                          <  3:378708/132‑1 (MQ=255)
                                                                                    ttGCCAGGCCGC‑C‑TTT‑TAA‑TCAGCCCGTTTTCTCCGCGTCCCCCCGAAATAACCCACGGCCGATTTCTTTTCTCGAAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGt                                          <  4:108444/138‑1 (MQ=255)
                                                                                    tgggcagccaGC‑GTTTT‑T‑A‑GCCGCCCGTTTTCTACGCCCCACCCCCCAACAAAACCCGCCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGt                                          <  8:345811/136‑1 (MQ=255)
                                                                                      ggcaggcaGCAT‑TTT‑T‑A‑GCAGCCCGTTTTATCCGCCGCACCCCGAAATAAAAGACGGCCTATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTca                                        <  4:222290/138‑1 (MQ=255)
                                                                                                               cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTTTGACAATATGAGTGAAAAAACCaa                   >  1:435171/1‑138 (MQ=255)
                                                                                                               cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc                  >  7:354598/1‑139 (MQ=255)
                                                                                                               cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc                  >  3:421628/1‑139 (MQ=255)
                                                                                                               cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc                  >  4:120136/1‑139 (MQ=255)
                                                                                                               cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAAc                  <  6:398337/139‑1 (MQ=255)
                                                                                                               cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACACATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATTAGAGTGAAAAAAGCaa                   >  4:43658/1‑138 (MQ=255)
                                                                                                               cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCGCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTGTTTGTAAAGATATTTGTGAAAAAACCAAc                  >  7:165177/1‑139 (MQ=255)
                                                                                                                               cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATTGAACGTGGAAAGAGAATTCATTTTCACAAAATGAGTGAAAAAACCAACTAGTTTTTAGAAGttt  >  8:54516/1‑139 (MQ=255)
                                                                                                                               cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTATAAGttt  >  2:178638/1‑139 (MQ=255)
                                                                                                                                       ||                                                                                                                                 
CGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGC‑C‑TTT‑T‑A‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTT  >  CP006881/3725109‑3725369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑