BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,067	1 bp→AT	100%	intergenic (‑181/‑120)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,066	1	.	A	97.4%	401.3 / 2.3	114	intergenic (‑180/‑121)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base . (0/3); new base A (37/74); total (37/77)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*	CP006881	3,723,067	0	G	T	97.4%	378.1 / 0.6	114	intergenic (‑181/‑120)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (37/74); minor base A (0/3); total (37/77)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 115 total aligned reads displayed.

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTT‑AGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCA  >  CP006881/3722935‑3723201

||

cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttt                                                                                                                                   <  1:246633/139‑1 (MQ=255)

cGAATAAACGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGGTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttt                                                                                                                                   <  5:276640/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTaaa                                                                                                                           <  2:311176/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTaaa                                                                                                                           <  8:177971/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTaaa                                                                                                                           <  5:30774/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTaaa                                                                                                                           <  1:406558/139‑1 (MQ=255)

        ggACACAGCGATCCTTTTTCATATACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTAATCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCATCTATTTTTTT‑AGTTaaa                                                                                                                           <  6:338466/139‑1 (MQ=255)

           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAg                                                                                                                        <  4:284483/139‑1 (MQ=255)

           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAg                                                                                                                        >  5:161723/1‑139 (MQ=255)

           cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAg                                                                                                                        >  4:45149/1‑139 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCTCATATTCAGAATAAGAAAACGCTATCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATTGt                                                                                                                       <  6:392949/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGt                                                                                                                       <  5:100163/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGt                                                                                                                       <  2:126103/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCAAGATAGCGTTTTCGTCAACT‑ATTTTTTAAGTTAAATAGt                                                                                                                       <  5:147516/139‑1 (MQ=255)

            acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACAAGACACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGATTTCTTCAACTATTTTTTT‑AGTTAAATatt                                                                                                                       >  6:147516/1‑137 (MQ=255)

            acaGCCTTCCTTTTTCATTTACGCAAATTTTACGTCTTGCCTGCTTTAACAGTCACAATCCCGCTTATTCAGTTTAAGAATACGCTTTCATGATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGt                                                                                                                       <  6:150271/139‑1 (MQ=255)

              aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGttt                                                                                                                     <  5:399801/139‑1 (MQ=255)

                cGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGa                                                                                                                   >  6:296703/1‑139 (MQ=255)

                cGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGa                                                                                                                   >  7:12167/1‑139 (MQ=255)

                   tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATat                                                                                                                <  1:77185/139‑2 (MQ=255)

                    ccTTTTTCATTTAAGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATat                                                                                                                <  8:192297/138‑2 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTaaa                                                                                                                           <  5:437853/123‑1 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTaaa                                                                                                                           >  6:437853/1‑123 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAAcat                                                                                                           >  2:347190/1‑137 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAAcat                                                                                                           >  1:415597/1‑137 (MQ=255)

                        tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGAAAGCGTTTTCGTCAACTATTTTTTT‑ATTTAAATATTTTGATATAAcat                                                                                                           >  2:178085/1‑137 (MQ=255)

                                          tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTa                                                                                          <  6:386135/138‑1 (MQ=255)

                                               cTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaat                                                                                     >  3:339976/1‑137 (MQ=255)

                                                ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                                    >  2:95456/1‑136 (MQ=255)

                                                ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                                    >  3:143029/1‑136 (MQ=255)

                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                  <  2:219555/138‑2 (MQ=255)

                                                  gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                  <  2:415597/138‑2 (MQ=255)

                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             >  1:76284/1‑138 (MQ=255)

                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             <  3:102791/138‑1 (MQ=255)

                                                       cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                             >  3:123460/1‑138 (MQ=255)

                                                          tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                          <  4:326632/138‑1 (MQ=255)

                                                          tACCAGTCACAATCACGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                          <  1:178085/138‑1 (MQ=255)

                                                            ccAGTCGCAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  3:38978/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCATATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  5:407183/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  8:429477/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  8:272588/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  5:83365/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                        <  1:277308/138‑3 (MQ=255)

                                                            ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                                                       >  5:187680/1‑139 (MQ=255)

                                                             cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                                      <  8:358664/139‑1 (MQ=255)

                                                                tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   <  1:220848/139‑1 (MQ=255)

                                                                tCACAATCACGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   <  6:55624/139‑1 (MQ=255)

                                                                 cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   >  2:384614/1‑138 (MQ=255)

                                                                 cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                   >  2:407211/1‑138 (MQ=255)

                                                                  acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAAATAATTGTGAAATACTTcaca                                                                 <  4:156574/139‑1 (MQ=255)

                                                                     ataccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:193760/136‑1 (MQ=255)

                                                                     atacagCTTATTCAGATTAAGAATAAGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  1:322376/134‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:424888/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:23179/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:422336/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:93211/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  2:180610/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:221916/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:187680/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:296703/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  5:292345/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:12167/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:176919/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  1:11180/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:353252/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:717/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:207786/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  4:123460/139‑1 (MQ=255)

                                                                     aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:7254/139‑1 (MQ=255)

                                                                      tCCCGCTTATTCAGATTAAGACTACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAatat                                                             <  3:289960/139‑1 (MQ=255)

                                                                      tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                          >  3:76902/1‑126 (MQ=255)

                                                                      tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                          <  4:76902/126‑1 (MQ=255)

                                                                        ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGAGGTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                            <  2:439356/138‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         <  1:384614/139‑1 (MQ=255)

                                                                          gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                         >  7:417750/1‑139 (MQ=255)

                                                                           cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  6:424242/133‑1 (MQ=255)

                                                                           cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  5:424242/1‑133 (MQ=255)

                                                                              aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  8:266223/1‑130 (MQ=255)

                                                                              aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  7:266223/130‑1 (MQ=255)

                                                                                tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  4:317774/1‑128 (MQ=255)

                                                                                tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  3:317774/128‑1 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                  <  1:363975/104‑2 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                                                                  >  2:363975/1‑103 (MQ=255)

                                                                                    aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                  <  4:318275/136‑1 (MQ=255)

                                                                                        agaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTc                                            <  5:220755/138‑1 (MQ=255)

                                                                                            tACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCa                                       <  1:407211/139‑1 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  8:72915/114‑1 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  7:72915/1‑114 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCTTGCTATACTATTCTCaataat                                      >  4:237837/1‑134 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                      >  7:21506/1‑138 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                      >  6:128506/1‑138 (MQ=255)

                                                                                              cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                      >  1:97753/1‑138 (MQ=255)

                                                                                                   tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  1:185563/1‑109 (MQ=255)

                                                                                                   tcatcaTAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  2:185563/109‑1 (MQ=255)

                                                                                                    catcatAAATCATGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaa                               >  5:35170/1‑139 (MQ=255)

                                                                                                            atcaTGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa                       <  3:45149/136‑1 (MQ=255)

                                                                                                               aTGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              >  8:142501/1‑97 (MQ=255)

                                                                                                               aTGATAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                              <  7:142501/97‑1 (MQ=255)

                                                                                                                  aTAGCGTTTTCGTCAACTATTTTTTT‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGAc                  <  3:38189/138‑1 (MQ=255)

                                                                                                                                 actattttttt‑AGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa  <  3:237837/134‑1 (MQ=255)

||

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTT‑AGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCA  >  CP006881/3722935‑3723201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`