| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,877,344 | (C)9→8 | intergenic (‑14/+58) | U712_14845 ← / ← U712_14850 | Uncharacterized protein ytnP/tRNA (guanine‑N(7)‑)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,877,336 | 0 | C | . | 94.1% | 203.4 / 5.0 | 51 | intergenic (‑6/+66) | U712_14845/U712_14850 | Uncharacterized protein ytnP/tRNA (guanine‑N(7)‑)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base . (28/20); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTCATTGACTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCACCCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATATT > CP006881/2877200‑2877469 | ttttCATTGACTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcc < 2:274779/138‑1 (MQ=255) tttCATTGACTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCAGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcacc > 8:68085/1‑139 (MQ=255) ttCATTGACTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcccc < 6:445022/138‑1 (MQ=255) ttCATTGACTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcccc < 7:113016/138‑1 (MQ=255) aTTGACTGGATATTTTTTTGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTACATATGCGTCACGCCTCCGTCAAGCCAGGGCAACGTGATGTTTCCAATTTTCATTGTTTCCTTTTTCAccccccc < 8:211757/138‑1 (MQ=255) aTTGACTGGATATTTTTTAGACCAAAGCGGCGTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAccccccc < 8:37655/138‑1 (MQ=255) ttGACTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcccccccct < 5:146621/139‑2 (MQ=255) aCTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGGCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcccccccctat < 2:28429/138‑4 (MQ=255) gATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTGCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTAtcatca < 4:112993/139‑1 (MQ=255) aGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCaaa > 6:177516/1‑139 (MQ=255) aGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCGATCATCATGGTAGCaaa > 4:444456/1‑139 (MQ=255) cGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCACCCCCCCCCTATCATCATGGTAGCAAACGGCCCAt < 1:33778/139‑1 (MQ=255) cTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATaaa > 8:403922/1‑138 (MQ=255) ggCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CACCCCCCGATCATCATGGTAGCAAACGGCCCATaaaaaaa > 7:55195/1‑138 (MQ=255) agccgcaaCAGGGCGCCTCGGTCCATATGCTGAACGCCTCCGTCAACCCAGGTCAACGTGTTGTTTCCTTTTTTCATTGTTTCCATTTTC‑CCCCCCCCTTATATTCTTGTTAGCTAAGGGCCCATAAAAAAATCAGACt < 2:397792/133‑1 (MQ=255) aCGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑‑CCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTc > 8:353588/1‑139 (MQ=255) aCGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCAGATTCA‑CCCCCCCCTCTCATCATGGGAGCCAACGGCCCGTAAAAAAAACAgccg > 8:84895/1‑136 (MQ=255) ctccGAACATGGCGCCTCCTTCCATATTCGTCACTCCTCCTTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATAATCATGGTAGAAAACGTACCATAAAAAAAACAGACTc < 3:383134/137‑1 (MQ=255) cGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTAGCATCATTGTAGCAAACGGCCCATAAAAAAAAAATACt > 1:316361/1‑138 (MQ=255) gAACATGGCGCCTCCGGCCATATGCGTCACGCCTCCGGCAAGCCAGGGCAACGTGATGTTGCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTCTCCTCATGGTCGCCCACGGCCCATAAAAAAAACAGACTCCTGt < 7:84895/139‑1 (MQ=255) aaCATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcc < 3:56356/87‑1 (MQ=255) aaCATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcc > 4:56356/1‑87 (MQ=255) aaCATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCCTGGTAGGAAACGGCCCATAAAAAAAACAGACTCCTGtg > 5:140117/1‑138 (MQ=255) ggCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTc < 2:269924/129‑1 (MQ=255) ggCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTc > 1:269924/1‑129 (MQ=255) ggCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAgt > 7:411455/1‑139 (MQ=255) ggCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAgt > 6:204481/1‑139 (MQ=255) ggCGCCTCCGTCCATATGCGCCACGCCTCCGGCAAGCCAGGCCAACGTGATGTTTCCAATTTTCATTGTTACAATTTTCA‑CCCCCCCCTATCAGCATGGAAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAgt < 3:112929/139‑1 (MQ=255) gcgcCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAgt < 2:114223/138‑1 (MQ=255) gcgcCACCGTCCATATGCGGCCCGCCTGCGTCCAGCCCGGTCAACGGGTTGTTTCCAATTTTGATTGTTTCCATTTTCA‑ACCCCCCCTATCATCATGGAAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAgt < 3:126536/138‑1 (MQ=255) atatGCGTCACGCCTCCGTCAAGCCCGGGCAACGGGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGtt < 8:323309/139‑1 (MQ=255) atatGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGtt < 8:357386/139‑1 (MQ=255) tGCGTCACGCCTCCGGCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTtctc < 2:324105/139‑1 (MQ=255) tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTAc < 4:372017/129‑1 (MQ=255) tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTAc > 3:372017/1‑129 (MQ=255) tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAtt > 3:87510/1‑139 (MQ=255) tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACACCTGTCAGAGTCTGTCTTTACGTTCTCCAt > 5:107705/1‑138 (MQ=255) tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGAAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAtt < 5:240937/139‑1 (MQ=255) tCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATAATCATGGTAGCTAACGGCCCAAAAAAAAAAAAGACTCCTGTCAGGGTGGGTAGTTACGTTCTACAt > 7:394904/1‑138 (MQ=255) ccACGCCTCCGTCAAGCCATCTCAACTTAATGTTTACAAGTTTCATTTTTTCCTTTTTCA‑CCCCACCCTATCATCATGGAATCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCAtt < 5:40191/138‑1 (MQ=255) cggCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑ACCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTc < 7:327711/136‑1 (MQ=255) cTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAcc < 5:307781/56‑1 (MQ=255) cTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCAca > 6:307781/1‑55 (MQ=255) cTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCt < 5:177516/139‑1 (MQ=255) cggacaGCCAGGTAAACGTGAGGTTTGAAATTTTCATTTTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAg < 7:389301/134‑1 (MQ=255) cGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAg > 8:127031/1‑139 (MQ=255) cGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAg > 5:212188/1‑139 (MQ=255) cGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGGAGCAAACGGCCCATAAAAAAAACAGCATCCGGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCACAg > 2:328534/1‑139 (MQ=255) cGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCAGCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTGTTTACGTTCTCCATTCAACCTCAg > 1:160292/1‑139 (MQ=255) gTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTGCTGTCAGAGTCTGTCTTTACGGTCTCCATTCCACCTCAGCCCGa > 1:83893/1‑133 (MQ=255) gTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTAACATCATGTTAGCAAACTGCCCATAAAAAAAACAGACTCATGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGa > 2:27151/1‑133 (MQ=255) cGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTg > 3:184223/1‑139 (MQ=255) cGTGATGTTTCCAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTg > 1:127884/1‑139 (MQ=255) ccAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCGATCATCATGGTTGCAAACGGCCCATAAAAAAAACAGACTCCGGTCAGAGTCTGTCTTTTGGTTCTCCAGTCAACCTCAGCCCGAGAGATCGGCTGGCCggggg > 6:447422/1‑133 (MQ=255) ccAATTTTCATTGTTTCCATTTTCA‑CCCCCCCCGATCATCATGGTAGCAAACGGCCCATAAAAAAAACACACTCCTGTCATAGTCTGTCTTTACGTTATCCATTCAAACTCAGCCCGATCGATAGGCTGGCCGAGTg > 8:383825/1‑137 (MQ=255) aTTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAAtttt > 5:217928/1‑139 (MQ=255) aTTGTTTCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAAtttt < 4:184223/139‑1 (MQ=255) aTTGTTACCATTTTC‑CCCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAAttt < 5:15896/138‑1 (MQ=255) tCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCATAGTCTGTCTTTACGTTATCCATTAAACATAAGCCCGATAGATCGCCTGGCCGAGTGCAGAGAATTTTTCTTCa > 2:21181/1‑139 (MQ=255) tCCATTTTCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCa > 8:132170/1‑139 (MQ=255) ttttCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATAtt < 5:213952/139‑1 (MQ=255) ttttCA‑CCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATAtt < 5:204481/139‑1 (MQ=255) ttttCA‑CCCCCCCATATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGTGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATAtt < 8:394904/139‑1 (MQ=255) ttttccccccccccTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCGCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATAtt < 6:140117/134‑1 (MQ=255) | TTTTCATTGACTGGATATTTTTTAGACCAAAGCGGCTTTGGCACAACGCCGAACATGGCGCCTCCGTCCATATGCGTCACGCCTCCGTCAAGCCAGGTCAACGTGATGTTTCCAATTTTCATTGTTTCCATTTTCACCCCCCCCCTATCATCATGGTAGCAAACGGCCCATAAAAAAAACAGACTCCTGTCAGAGTCTGTCTTTACGTTCTCCATTCAACCTCAGCCCGATAGATCGGCTGGCCGAGTGCAGAGAATTTTTCTTCATATT > CP006881/2877200‑2877469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |