| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,191,772 | +C | coding (225/648 nt) | U712_10980 ← | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,191,772 | 1 | . | C | 97.7% | 133.8 / ‑2.7 | 43 | coding (225/648 nt) | U712_10980 | N‑(5'‑phosphoribosyl)anthranilate isomerase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (26/16); total (27/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
AAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATA > CP006881/2191637‑2191907 | aaaGCGGGCTATTTCTTGAGTTGTGTTATCTTGATGGTGAAGCGCCGTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACTTCGAGCTTCAAgct > 2:157108/1‑136 (MQ=255) tttctTGAGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAAtac < 4:295473/136‑3 (MQ=255) aGTTGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt < 2:348446/138‑1 (MQ=255) aGTGGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt < 4:377026/138‑1 (MQ=255) ttGTGTTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGt < 6:52877/139‑1 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt < 1:258117/133‑1 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACAt > 2:258117/1‑133 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc > 7:315092/1‑139 (MQ=255) tgtTATCTTGATGGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGtctc > 2:7885/1‑139 (MQ=255) tcttgatgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTAttg > 7:242276/4‑137 (MQ=255) atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATATGTGACATCGTCTCTATTGATTCa > 7:212658/1‑139 (MQ=255) atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa > 7:49407/1‑139 (MQ=255) atgGTGAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCa > 7:302110/1‑139 (MQ=255) gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGCATGACGTCGAGCTTCACGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa > 2:153766/1‑138 (MQ=255) gAAGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTaa > 5:240122/1‑138 (MQ=255) acgcgcCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCCTCCCCGTGAAGATGAATGACGTAGCGCTTCAAGCTCGT‑GTCAATACGTGACTTCGTCTCTATTTATTCAATAAc > 4:236920/3‑138 (MQ=255) aaGCGCCTTCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGATTCAAGCTCTT‑GGCAATACGTGACATCGACTCTATTGATTCAATAAc > 8:6557/1‑138 (MQ=255) tCCATATTTCACAGCCTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAAcacc > 5:118448/1‑139 (MQ=255) ttCACAGCCTGTCAGCTTGCGAAGCGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATGCATTAACAAAAACACCGGCAAcc > 7:397797/1‑139 (MQ=255) agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc < 8:140074/130‑1 (MQ=255) agccTGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATAACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAAc > 7:140074/4‑133 (MQ=255) tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACTGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc > 5:335189/1‑138 (MQ=255) tGTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTc > 7:391604/1‑138 (MQ=255) gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt > 6:123200/1‑136 (MQ=255) gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGttttt < 5:123200/136‑1 (MQ=255) gTCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa > 1:229942/1‑139 (MQ=255) tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAGGCTCTT‑GGCATTACGTGACATCGTCTCGATTGATTCATTAGCAAAAACACCTGCACCCTGTTTTTCGac > 2:287172/1‑139 (MQ=255) tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa < 2:413446/138‑1 (MQ=255) tCAGCTTGCGAAGAGCAGCAGCATCCGCCGGTT‑TTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa < 8:170366/137‑1 (MQ=255) tCAGCTTGCGAACAGCAGCAGCATCCGCCTGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGa < 6:13699/138‑1 (MQ=255) aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGATGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac < 1:157108/139‑1 (MQ=255) aGCTTGCGAAGAGCAGCAGCATCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGacac < 8:391604/139‑1 (MQ=255) tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 4:370674/1‑139 (MQ=255) tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 1:250719/1‑139 (MQ=255) tCCGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATtttt > 1:226304/1‑139 (MQ=255) cGCCGGTTTTTCATCACCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTc < 6:118448/139‑1 (MQ=255) gtttttcatcaCTGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATc < 6:335189/134‑1 (MQ=255) catcaCCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCg < 8:49407/138‑1 (MQ=255) tcaCCGTGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGgag < 4:296936/139‑1 (MQ=255) tGAAGCTGAATGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACtt > 1:180546/1‑139 (MQ=255) aaTGACGTCGAGCTTCAAGCTCTT‑GGCATTACGTGACATCGTCTCTATTGATTCATTAACAAAAACCCCTGCACCCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCgtttg > 7:13165/1‑135 (MQ=255) aaTGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATGATCCGGAGATACTTTTCgtttg > 4:185505/1‑135 (MQ=255) tGACGTCGAGCTTCAAGCTCTT‑GGCAATACGTGACATCGTCTCTATTGATTCATTAACAAAAACACCTGCAACCTGTTTTTCGACACGAACTTGGTTCAGCCATTTTTTCACATCTTCCGGAGATACTTTTCGTTTGCt > 1:129384/1‑139 (MQ=255) aaG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATa > 4:100151/1‑138 (MQ=255) | AAAGCGGGCTATCTCTTGAGTTGTGTTCTCATGATGGTGAAGCGCCTTCCATATTTCACAATCTGTCAGCATGCGAAGCGCATCGGCATCCGCAGGTTCCTCATCACCGTGAAGCTGAATGACGTCTAGATTTAAG‑TCTTCGGCAATCCGTGACATCGTCTCTATCGATTCATTAACAAAAACCCCTGCAACCTGTTTTTCGACACAAACTTGACTCAGCCACTTTTTCACATCTTGCGGAGATACTTTTCGGTAGCTTTCAGCAAAAATA > CP006881/2191637‑2191907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |