Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,173,196 T→C L184L (TTA→TTG)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,173,1960TC97.1% 215.8 / ‑4.3 70L184L (TTA→TTG) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (33/35);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCCTTCAGTTCATCTGGAAAATCATCAGACTGCTGGTTAACGTTAATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCT  >  CP006881/2173063‑2173330
                                                                                                                                     |                                                                                                                                      
tGTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATGACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAat                                                                                                                                   <  8:256821/139‑1 (MQ=255)
 gTCCTTCAATTCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAata                                                                                                                                  >  2:112803/1‑139 (MQ=255)
       caattCATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAttt                                                                                                                            >  1:302026/4‑138 (MQ=255)
            cATCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGcc                                                                                                                        <  7:42391/138‑1 (MQ=255)
             aTCTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCCGCCGGCCTTTCCGATAGGATACCGCCTGTGTTTTTTCCGTTAAGCAAAATATCATTTGGCCag                                                                                                                      >  1:283486/1‑138 (MQ=255)
               cTGGAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCAttt                                                                                                                    >  2:312852/1‑139 (MQ=255)
                  gAAAATCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGCCTGTGTTTTTTCCGTTAATCAAAATTTCATTTGGCCATTTaa                                                                                                                  >  7:311902/1‑138 (MQ=255)
                   aaaaTCATTAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAata                                                                                                                <  6:403864/139‑1 (MQ=255)
                      atcattAGGCTGCTGGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATcc                                                                                                             <  2:4726/139‑1 (MQ=255)
                         attaggctgctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGt                                                                                                           <  8:317489/132‑1 (MQ=255)
                             ggctgctgGTTAACGTTATTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATTCCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGa                                                                                                      <  6:145175/137‑1 (MQ=255)
                                  ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGc                                                                                                                         >  7:269824/1‑115 (MQ=255)
                                  ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGc                                                                                                                         <  8:269824/115‑1 (MQ=255)
                                  ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct                                                                                                 >  2:24451/1‑139 (MQ=255)
                                  ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct                                                                                                 >  2:20125/1‑139 (MQ=255)
                                  ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct                                                                                                 <  1:228886/139‑1 (MQ=255)
                                  ctgGTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATAATTTGGCCATTTAATATCCTTTTGGATGCct                                                                                                 >  7:148667/1‑139 (MQ=255)
                                     gTTAACGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCctgct                                                                                              <  6:81155/139‑1 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct                                                                                                 <  7:16554/133‑1 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCct                                                                                                 >  8:16554/1‑133 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  4:217751/138‑1 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  8:137412/138‑1 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  2:36398/138‑1 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  3:15395/138‑1 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  1:112803/138‑1 (MQ=255)
                                        aaCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  7:205954/138‑1 (MQ=255)
                                         aCGTTAATGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGcct                                                                                          <  8:135781/139‑2 (MQ=255)
                                               aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGGATTTGCGTTATGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAGTTGGCCATATAATATCCGTTTGGTTTCCTGCTGCctctgcg                                                                                     >  4:262231/1‑135 (MQ=255)
                                               aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAatat                                                                                                               <  6:239164/112‑1 (MQ=255)
                                               aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAatat                                                                                                               >  5:239164/1‑112 (MQ=255)
                                               aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTa                                                                                    >  5:207600/1‑139 (MQ=255)
                                               aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTa                                                                                    <  1:312852/139‑1 (MQ=255)
                                                     aTCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGATTGGATTCCCt                                                                              >  7:255523/1‑139 (MQ=255)
                                                     aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGCCTGTTTTTTTTCCGTTAATCAAAATATCATGTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCGATTCCCt                                                                              >  2:152074/1‑139 (MQ=255)
                                                     aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCt                                                                              >  2:51337/1‑139 (MQ=255)
                                                     aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCt                                                                              >  8:309226/1‑139 (MQ=255)
                                                            tgatCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                       <  2:302026/139‑1 (MQ=255)
                                                            tgatCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                       <  2:260607/139‑1 (MQ=255)
                                                                 aCTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGTTACCGGCTGGTTTTTTTACGTTAATCAAAATATCATTTGGCCATTTTATATCCGTTTGGATGCCTGCTGCCTCGTCTTTTCCCTGCCCCACAGCTa                                                                  >  4:9052/1‑139 (MQ=255)
                                                                 aCTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACAGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGTCCATTTAATATCCGTATTGATGCATGCTGACTATTCTATTACCTGCACAACAGCTa                                                                  >  8:19510/1‑139 (MQ=255)
                                                                     aacGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTg                                                               >  8:410592/3‑138 (MQ=255)
                                                                          aCGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTcc                                                                                <  6:390717/116‑1 (MQ=255)
                                                                          aCGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAATTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTcc                                                                                >  5:390717/1‑116 (MQ=255)
                                                                             cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            >  4:116365/1‑101 (MQ=255)
                                                                             cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  3:116365/101‑1 (MQ=255)
                                                                             cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                          >  5:208091/1‑119 (MQ=255)
                                                                             cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                          <  6:208091/119‑1 (MQ=255)
                                                                             cgGTCTTCTTCAGCCTGCATTTCCGGTTGGATACCGCCTGGTTTTTTTCCGGTAATCAAAATATCATTTGGCCATTTTATATGCGTTTGGATGCCTGCTGCGTCGTGTATTCCCTGCACAACAGCTACTTCAGCAAg                                                        >  7:222228/1‑137 (MQ=255)
                                                                               gTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGTATGCCtgctgc                                                                                            >  6:217721/1‑99 (MQ=255)
                                                                               gTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCtgctgc                                                                                            <  5:217721/99‑1 (MQ=255)
                                                                                         gCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCg                                          <  5:148001/139‑1 (MQ=255)
                                                                                          ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                          <  1:49798/106‑1 (MQ=255)
                                                                                          ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                          >  2:49798/1‑106 (MQ=255)
                                                                                          ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCgg                                         >  5:279196/1‑139 (MQ=255)
                                                                                              cATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGGGtt                                     >  5:69217/1‑139 (MQ=255)
                                                                                                  tccgTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGttttt                                  <  3:25163/135‑1 (MQ=255)
                                                                                                        aggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGgt                                        >  1:126118/3‑126 (MQ=255)
                                                                                                        aggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGgt                                        <  2:126118/124‑1 (MQ=255)
                                                                                                         ggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCg                           <  8:170068/137‑1 (MQ=255)
                                                                                                                 aCTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                         >  8:165375/1‑84 (MQ=255)
                                                                                                                 aCTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                         <  7:165375/84‑1 (MQ=255)
                                                                                                                    gTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                         <  4:216485/81‑1 (MQ=255)
                                                                                                                    gTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                         >  3:216485/1‑81 (MQ=255)
                                                                                                                     ttttttttCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                       <  5:42520/82‑1 (MQ=255)
                                                                                                                     ttttttttCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                       >  6:42520/1‑82 (MQ=255)
                                                                                                                     ttttttttCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTTGAGAGGAATGTCAGGccc              >  4:154557/1‑138 (MQ=255)
                                                                                                                       ttttttCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGc             <  8:255523/138‑1 (MQ=255)
                                                                                                                               gTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCctgct                                                                                              >  3:277118/1‑49 (MQ=255)
                                                                                                                               gTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCctgct                                                                                              <  4:277118/49‑1 (MQ=255)
                                                                                                                               gTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAgg    <  5:127712/139‑1 (MQ=255)
                                                                                                                                  aatcaaAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCt  <  4:212650/138‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TGTCCTTCAGTTCATCTGGAAAATCATCAGACTGCTGGTTAACGTTAATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCT  >  CP006881/2173063‑2173330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑