| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,188,981 | G→A | G126G (GGC→GGT) | U712_10965 ← | Histidinol‑phosphate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,188,981 | 0 | G | A | 96.7% | 165.5 / ‑2.5 | 61 | G126G (GGC→GGT) | U712_10965 | Histidinol‑phosphate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (29/29); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.06e-01 | |||||||||||
CTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTATCATTTAAAAATGCCCGGCAGATGATCTGAATGATTTCATCTGAACCGTTTCCGAAAAT > CP006881/2188845‑2189106 | cTTCAGATGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCAcc > 3:28152/1‑139 (MQ=255) tGTATAAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAAta < 3:192326/139‑2 (MQ=255) taAGTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCg > 6:213581/1‑139 (MQ=255) gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt < 5:32496/139‑1 (MQ=255) gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt < 6:222438/139‑1 (MQ=255) gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt < 8:201582/139‑1 (MQ=255) gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt < 4:28152/139‑1 (MQ=255) gTGCCAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt < 3:224598/139‑1 (MQ=255) gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg > 4:155194/1‑130 (MQ=255) gCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATg < 3:155194/130‑1 (MQ=255) cAGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGATGTGCAAATTCACGTACTTCAGCACCTTCAATAACCGCATTaag > 3:88293/1‑137 (MQ=255) aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt > 8:305377/1‑139 (MQ=255) aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt > 5:401940/1‑139 (MQ=255) aGTCGGATTATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGt > 4:253402/1‑139 (MQ=255) gattATTCGGGATGCATATCCAAACGACTTTTGTCTGTTAGTCGATTGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTAGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTtata < 2:432680/137‑1 (MQ=255) attATTCGGGCTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAAGCATGTGAGCCGTCGGGACGAAGTGCAAGTTAACGTACTTCAGCACCTTCAATAACCGCATTATGTTTatat > 7:260716/2‑139 (MQ=255) gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCa > 4:355777/2‑118 (MQ=255) gcTGCATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCa < 3:355777/117‑1 (MQ=255) cATATCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt < 3:253402/137‑1 (MQ=255) atatCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCATCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTggg > 4:331805/1‑139 (MQ=255) tCCACACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc < 7:176394/139‑1 (MQ=255) tCCAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc < 7:182176/139‑1 (MQ=255) gccAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc < 3:164438/138‑1 (MQ=255) cAAACGACTTGTGTCTGTTCGTCGATGGCTTCTAACATCGCGGCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCTTTATGTTTATATTGCGGAAAAGTGGGGgcag < 7:33512/139‑1 (MQ=255) aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt < 4:100699/107‑1 (MQ=255) aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCAt > 3:100699/1‑107 (MQ=255) aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt < 1:390101/129‑1 (MQ=255) aaCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGt > 2:390101/1‑129 (MQ=255) aaCGACTTGTGTCTGTGCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgcagca < 8:20380/139‑1 (MQ=255) aCGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGcagcag < 2:403055/139‑1 (MQ=255) cGACTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCAAGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGcagcag > 2:100956/1‑138 (MQ=255) cTTGTGTCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGtaac < 5:213581/139‑4 (MQ=255) gtgtCTGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGAAATTTAACGTACATCCTCACCTTCAAATACCGCATTATGTTTATATTTCGGAAAAGTTGGGGCAGCAGtacacgc > 4:164438/1‑133 (MQ=255) tGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTAAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGtttg > 6:393617/1‑137 (MQ=255) ttCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCCCGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGgttgtt > 7:412948/1‑139 (MQ=255) gTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa < 7:290499/139‑1 (MQ=255) cgatggCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATc > 6:307260/6‑139 (MQ=255) gCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAAACGCATTATGTTTATATTTCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGttt > 5:123336/1‑138 (MQ=255) aTGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGccc < 1:38831/139‑1 (MQ=255) ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCgtgt > 7:367766/1‑113 (MQ=255) ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCgtgt < 8:367766/113‑1 (MQ=255) ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCgg > 4:424040/1‑139 (MQ=255) ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCgg > 2:49612/1‑139 (MQ=255) gcgtCTAAATCCTGTGAGCCGGCGGGACGCAGTGCAATTTCACGTGCTTCAGCCCCTTCAATACCCGCATTAGGTTTATATTGCGGAAAAGTGGGGGCAGCAGTCTCCGTGTTTGGTTTTTGGTTTAATAATGCCCGGc > 8:191075/4‑139 (MQ=255) gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa > 4:417570/4‑120 (MQ=255) gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa < 3:417570/117‑1 (MQ=255) gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCgg > 5:586/4‑138 (MQ=255) gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGc < 3:331805/136‑1 (MQ=255) tAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAga < 4:102730/137‑1 (MQ=255) tAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAga > 3:102730/1‑137 (MQ=255) aTGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTgt < 3:424040/139‑2 (MQ=255) tgtgAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTgta > 3:378977/1‑137 (MQ=255) gtgAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc < 2:100150/85‑1 (MQ=255) gtgAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGgc > 1:100150/1‑85 (MQ=255) gtgAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTAt > 2:312678/1‑139 (MQ=255) aGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGAt < 7:331956/139‑1 (MQ=255) aGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGAt < 1:100956/139‑1 (MQ=255) aGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGt > 4:349242/1‑139 (MQ=255) caaTTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGcc < 1:49612/137‑1 (MQ=255) cACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGaaaa > 6:139654/1‑138 (MQ=255) cgtaCTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATCATCTGTATGATTTCATCAGAACCGTTGCCGAAAAt > 5:154942/4‑137 (MQ=255) | CTTCTGATGTATAAGTGCCAGTCGGGTTATTCGGACTGCATATCCAAACGACTTGTGTCTGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTATCATTTAAAAATGCCCGGCAGATGATCTGAATGATTTCATCTGAACCGTTTCCGAAAAT > CP006881/2188845‑2189106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |