Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,189,032 T→A I109V (ATA→GTT)  U712_10965 ← Histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,189,0320TA98.7% 231.8 / NA 74I109V (ATA→GTT) U712_10965Histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (37/36);  minor base C (1/0);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTATCATTTAAAAATGCCCGGCAGATGATCTGAATGATTTCATCTGAACCGTTTCCGAAAATAAGTGATGTTTCACTGACATTGAGGTGCTCACTGAGCCTTGTCCGCAAAGCGGCGCTATA  >  CP006881/2188904‑2189166
                                                                                                                                |                                                                                                                                      
tGTTCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTAAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGtttg                                                                                                                              >  6:393617/1‑137 (MQ=255)
  ttCGTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCCCGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGgttgtt                                                                                                                            >  7:412948/1‑139 (MQ=255)
     gTCGATGGCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa                                                                                                                         <  7:290499/139‑1 (MQ=255)
       cgatggCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATc                                                                                                                       >  6:307260/6‑139 (MQ=255)
            gCTTCTAACATGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAAACGCATTATGTTTATATTTCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGttt                                                                                                                   >  5:123336/1‑138 (MQ=255)
                     aTGGCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGccc                                                                                                         <  1:38831/139‑1 (MQ=255)
                       ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCgtgt                                                                                                                                 <  8:367766/113‑1 (MQ=255)
                       ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCgtgt                                                                                                                                 >  7:367766/1‑113 (MQ=255)
                       ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCgg                                                                                                       >  4:424040/1‑139 (MQ=255)
                       ggCGTCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCgg                                                                                                       >  2:49612/1‑139 (MQ=255)
                        gcgtCTAAATCCTGTGAGCCGGCGGGACGCAGTGCAATTTCACGTGCTTCAGCCCCTTCAATACCCGCATTAGGTTTATATTGCGGAAAAGTGGGGGCAGCAGTCTCCGTGTTTGGTTTTTGGTTTAATAATGCCCGGc                                                                                                      >  8:191075/4‑139 (MQ=255)
                        gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa                                                                                                                         <  3:417570/117‑1 (MQ=255)
                        gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTa                                                                                                                         >  4:417570/4‑120 (MQ=255)
                        gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCgg                                                                                                       >  5:586/4‑138 (MQ=255)
                        gcgtCTAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGc                                                                                                      <  3:331805/136‑1 (MQ=255)
                             tAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAga                                                                                                   <  4:102730/137‑1 (MQ=255)
                             tAAATCATGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAga                                                                                                   >  3:102730/1‑137 (MQ=255)
                                   aTGTGAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTgt                                                                                           <  3:424040/139‑2 (MQ=255)
                                    tgtgAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTgta                                                                                          >  3:378977/1‑137 (MQ=255)
                                     gtgAGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTAt                                                                                         >  2:312678/1‑139 (MQ=255)
                                        aGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGAt                                                                                      <  7:331956/139‑1 (MQ=255)
                                        aGCCGTCGGGACGAAGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGAt                                                                                      <  1:100956/139‑1 (MQ=255)
                                                      aGTGCAATTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGt                                                                        >  4:349242/1‑139 (MQ=255)
                                                          caaTTTCACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGcc                                                                    <  1:49612/137‑1 (MQ=255)
                                                                cACGTACTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGaaaa                                                               >  6:139654/1‑138 (MQ=255)
                                                                  cgtaCTTCAGCACCTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATCATCTGTATGATTTCATCAGAACCGTTGCCGAAAAt                                                              >  5:154942/4‑137 (MQ=255)
                                                                           gcaccTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGttt                                                   >  4:41124/4‑139 (MQ=255)
                                                                              ccTTCAATAACCGCATTATGTTTATTTTGCGGAAAAGTGGGGTCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTcgc                                                <  5:391956/139‑3 (MQ=255)
                                                                               cTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTcgc                                                <  3:415773/138‑3 (MQ=255)
                                                                               cTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTcgc                                                >  4:415773/1‑136 (MQ=255)
                                                                               cTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCt                                               >  6:344300/1‑139 (MQ=255)
                                                                               cTTCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCt                                               >  8:328814/1‑139 (MQ=255)
                                                                                 tCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAgatgat                                                                                               <  4:218578/89‑1 (MQ=255)
                                                                                 tCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAgatgat                                                                                               >  3:218578/1‑89 (MQ=255)
                                                                                 tCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGa                                             >  6:109066/1‑139 (MQ=255)
                                                                                 tCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGa                                             >  4:334272/1‑139 (MQ=255)
                                                                                 tCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGa                                             >  5:420784/1‑139 (MQ=255)
                                                                                 tCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGa                                             >  3:338383/1‑139 (MQ=255)
                                                                                 tCAATAACCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGa                                             >  5:199296/1‑139 (MQ=255)
                                                                                     taaCCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACAtt                                         <  5:393617/137‑1 (MQ=255)
                                                                                     taaCCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACAtt                                         <  7:334439/137‑1 (MQ=255)
                                                                                       aCCGGATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGa                                       <  2:222914/139‑1 (MQ=255)
                                                                                       aCCGCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGa                                       <  7:328814/139‑1 (MQ=255)
                                                                                          gCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGt                                    <  8:243825/139‑1 (MQ=255)
                                                                                          gCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGt                                    <  1:312678/139‑1 (MQ=255)
                                                                                          gCATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAAACGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGt                                    <  8:80855/139‑1 (MQ=255)
                                                                                           caatATGTTTATATTGCGGAAAAGAGGGGGCAGCAGTAACAGTGTTTGTTTTATCGTATAAAAATGCCCGGCAGATGATCTGTATGATATCATCAGAACCGTTGCCGAAAATAAGTGAAGTTTCGCTGACATTGAgtt                                    >  1:222914/4‑136 (MQ=255)
                                                                                           cATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCTTGTTTGTTTTATCGTTTAAAAATGCACGGCAGATGATCTGTATGATTTCATCAGAACAGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTg                                   <  7:422259/139‑1 (MQ=255)
                                                                                           cATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCg                                                                         >  4:401383/1‑101 (MQ=255)
                                                                                           cATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCg                                                                         <  3:401383/101‑1 (MQ=255)
                                                                                           cATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTg                                   <  5:109066/139‑1 (MQ=255)
                                                                                           cATTATGTTTATATTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTg                                   >  5:397697/1‑139 (MQ=255)
                                                                                                     atatTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAgatg                                                                                                 >  3:88650/1‑67 (MQ=255)
                                                                                                     atatTGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAgatg                                                                                                 <  4:88650/67‑1 (MQ=255)
                                                                                                        ttGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCtt                      >  4:315037/1‑139 (MQ=255)
                                                                                                        ttGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCtt                      <  6:401940/139‑1 (MQ=255)
                                                                                                         tGCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGAGCTGTATGATTTCATCAGAACCGTGGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCtt                      <  7:367425/138‑1 (MQ=255)
                                                                                                          gCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAgatgat                                                                                               <  7:142899/64‑1 (MQ=255)
                                                                                                          gCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAgatgat                                                                                               >  8:142899/1‑64 (MQ=255)
                                                                                                          gCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATg                                                      >  1:408963/1‑105 (MQ=255)
                                                                                                          gCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATg                                                      <  2:408963/105‑1 (MQ=255)
                                                                                                          gCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACAtt                                         >  1:140362/1‑118 (MQ=255)
                                                                                                          gCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACAtt                                         <  2:140362/118‑1 (MQ=255)
                                                                                                          gCGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGt                    >  2:275734/1‑139 (MQ=255)
                                                                                                           cGGAAAAGTGGGGGCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTc                   >  7:50870/1‑139 (MQ=255)
                                                                                                                  gtgggggCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAAAg            <  5:307260/136‑1 (MQ=255)
                                                                                                                  gtgggggCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAAAg            <  1:275734/136‑1 (MQ=255)
                                                                                                                   tgggggCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAAAgc           <  4:338383/137‑1 (MQ=255)
                                                                                                                   tgggggCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAAAg            <  3:334272/136‑1 (MQ=255)
                                                                                                                   tgggggCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGGTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAACgc           <  4:88293/137‑1 (MQ=255)
                                                                                                                     ggggCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGC‑AAgcggc        >  7:32562/1‑139 (MQ=255)
                                                                                                                       ggCAGCAGTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAAAgcggcg       <  5:344300/139‑1 (MQ=255)
                                                                                                                          agcagTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGATGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAAAGCGGCGCt     >  4:138209/1‑138 (MQ=255)
                                                                                                                            cagTAACCGTGTTTGTTTTATCGTTTAAAAATGCCCGGCAGATGATCTGTATGATTTCATCAGAACCGTTGCCGAAAATAAGTGAGGTTTCGCTGACATTGAGGTGCTTACTGAGCCTTGTCCGCAAAGCGGCGCtata  <  8:412948/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                      
TGTTCGTCAATCGCTTCAAGCATGGCATCTAAATCATGTGAGCCGTCAGGACGAAGTGCGATTTCACGAACTTCAGCGCCTTCAATGACCGCATTATGTTTATATTGCGGAAAAGTCGGGGCAGCAGTTATCGTGTTAGTTTTATCATTTAAAAATGCCCGGCAGATGATCTGAATGATTTCATCTGAACCGTTTCCGAAAATAAGTGATGTTTCACTGACATTGAGGTGCTCACTGAGCCTTGTCCGCAAAGCGGCGCTATA  >  CP006881/2188904‑2189166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑