Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,173,242	G→T	A169E (GCG→GAG)	U712_10870 ←	Bifunctional protein BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,173,242	0	G	T	96.0%	132.8 / ‑4.7	50	A169E (GCG→GAG)	U712_10870	Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (20/28);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGA  >  CP006881/2173110‑2173372                                                                                                                                     |                                                                                                                                   aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGGATTTGCGTTATGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAGTTGGCCATATAATATCCGTTTGGTTTCCTGCTGCctctgcg                                                                                                                               >  4:262231/1‑135 (MQ=255) aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTa                                                                                                                              <  1:312852/139‑1 (MQ=255) aTGCCGATCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTa                                                                                                                              >  5:207600/1‑139 (MQ=255)       aTCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGATTGGATTCCCt                                                                                                                        >  7:255523/1‑139 (MQ=255)       aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGCCTGTTTTTTTTCCGTTAATCAAAATATCATGTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCGATTCCCt                                                                                                                        >  2:152074/1‑139 (MQ=255)       aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCt                                                                                                                        >  8:309226/1‑139 (MQ=255)       aTCCCAATGATCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCt                                                                                                                        >  2:51337/1‑139 (MQ=255)              tgatCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                                                                 <  2:260607/139‑1 (MQ=255)              tgatCACTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                                                                 <  2:302026/139‑1 (MQ=255)                   aCTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGTTACCGGCTGGTTTTTTTACGTTAATCAAAATATCATTTGGCCATTTTATATCCGTTTGGATGCCTGCTGCCTCGTCTTTTCCCTGCCCCACAGCTa                                                                                                            >  4:9052/1‑139 (MQ=255)                   aCTGAACGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACAGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGTCCATTTAATATCCGTATTGATGCATGCTGACTATTCTATTACCTGCACAACAGCTa                                                                                                            >  8:19510/1‑139 (MQ=255)                       aacGTACGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTg                                                                                                         >  8:410592/3‑138 (MQ=255)                            aCGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTcc                                                                                                                          <  6:390717/116‑1 (MQ=255)                            aCGCGGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCAATTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTcc                                                                                                                          >  5:390717/1‑116 (MQ=255)                               cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                                                                    <  6:208091/119‑1 (MQ=255)                               cgGTCTTCTTCAGCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                                                                    >  5:208091/1‑119 (MQ=255)                               cgGTCTTCTTCAGCCTGCATTTCCGGTTGGATACCGCCTGGTTTTTTTCCGGTAATCAAAATATCATTTGGCCATTTTATATGCGTTTGGATGCCTGCTGCGTCGTGTATTCCCTGCACAACAGCTACTTCAGCAAg                                                                                                  >  7:222228/1‑137 (MQ=255)                                           gCCTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCg                                                                                    <  5:148001/139‑1 (MQ=255)                                            ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                                                                    >  2:49798/1‑106 (MQ=255)                                            ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcac                                                                                                                    <  1:49798/106‑1 (MQ=255)                                            ccTGCATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCgg                                                                                   >  5:279196/1‑139 (MQ=255)                                                cATTTCCGTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGGGtt                                                                               >  5:69217/1‑139 (MQ=255)                                                    tccgTTAGGATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGttttt                                                                            <  3:25163/135‑1 (MQ=255)                                                          aggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGgt                                                                                  >  1:126118/3‑126 (MQ=255)                                                          aggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGgt                                                                                  <  2:126118/124‑1 (MQ=255)                                                           ggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCg                                                                     <  8:170068/137‑1 (MQ=255)                                                                   aCTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                                                                   <  7:165375/84‑1 (MQ=255)                                                                   aCTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                                                                   >  8:165375/1‑84 (MQ=255)                                                                      gTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                                                                   <  4:216485/81‑1 (MQ=255)                                                                      gTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGcaca                                                                                                                   >  3:216485/1‑81 (MQ=255)                                                                       ttttttttCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                                                                 <  5:42520/82‑1 (MQ=255)                                                                       ttttttttCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCacaac                                                                                                                 >  6:42520/1‑82 (MQ=255)                                                                       ttttttttCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTTGAGAGGAATGTCAGGccc                                                        >  4:154557/1‑138 (MQ=255)                                                                         ttttttCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGc                                                       <  8:255523/138‑1 (MQ=255)                                                                                 gTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAgg                                              <  5:127712/139‑1 (MQ=255)                                                                                    aatcaaAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCt                                            <  4:212650/138‑1 (MQ=255)                                                                                                             atatCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTga                   <  8:311902/138‑1 (MQ=255)                                                                                                             atatCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTga                   <  6:207600/138‑1 (MQ=255)                                                                                                             atatCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTga                   <  3:9052/138‑1 (MQ=255)                                                                                                              cagccGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTgaga                 <  7:297011/136‑1 (MQ=255)                                                                                                                tCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCCACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGaa                <  3:262231/138‑1 (MQ=255)                                                                                                                   gTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc            <  7:420153/139‑1 (MQ=255)                                                                                                                   gTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc            <  8:148667/139‑1 (MQ=255)                                                                                                                   gTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc            <  5:302749/139‑1 (MQ=255)                                                                                                                    tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACATCAGCTACTGCAGCAAGCAGAGTCAGATGCGGTGTTTTTTGGAGCGGAATGTGAGGCCGCAAAATCAGGCTCATCCCAACACCGTTTCCTTCTTGAGAATGGCa           >  2:246915/1‑139 (MQ=255)                                                                                                                    tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa           <  7:19510/139‑1 (MQ=255)                                                                                                                    tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa           <  7:309226/139‑1 (MQ=255)                                                                                                                    tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa           <  6:279196/139‑1 (MQ=255)                                                                                                                            ccTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCAACCAAACCCCCTTTCCTTCTTGAGAATGCCATAcccctg   >  8:297011/1‑136 (MQ=255)                                                                                                                             ctgctgCCTCTTCTATTCCCTGCACCACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATAcccctgt  <  1:152074/139‑5 (MQ=255)                                                                                                                             ctgctgCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCGCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGa  <  3:413945/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   ATGCCGATCCCGATGATAACTGACCGTACGCGGTCTTCCTCGGCCTGCATTTCGGTTAAGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGA  >  CP006881/2173110‑2173372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑