Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,189,382	A→G	Y264Y (TAT→TAC)	U712_10970 ←	Tryptophan synthase alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,189,382	0	A	G	100.0%	196.2 / NA	60	Y264Y (TAT→TAC)	U712_10970	Tryptophan synthase alpha chain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/31);  total (29/31)

TTCGTTTGAAGCAAGCTTCACAACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCAC  >  CP006881/2189245‑2189515                                                                                                                                          |                                                                                                                                      ctCGTTTGAAGCAAGCTTCACAACTTTATCAAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTGTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACtgta                                                                                                                                     >  8:34183/2‑139 (MQ=255)   cGTTTGAAGCAAGCTTCACAACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACa                                                                                                                                   <  8:55446/139‑1 (MQ=255)    gTTTGAAGCAAGCTTCACAACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACa                                                                                                                                   <  3:398055/138‑1 (MQ=255)             aagcTTCACAACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTaaa                                                                                                                         <  3:315037/139‑1 (MQ=255)             aagcTTCACAACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCGTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTaaa                                                                                                                         <  8:216567/139‑1 (MQ=255)              agcTTCACAACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAAc                                                                                                                        >  5:121450/1‑139 (MQ=255)                   cacaACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGcca                                                                                                                    <  3:364516/138‑2 (MQ=255)                   cacaACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGcca                                                                                                                    <  3:353788/138‑2 (MQ=255)                   cacaACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGcca                                                                                                                    <  7:380772/138‑2 (MQ=255)                             tCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATcc                                                                                                         >  4:403773/1‑138 (MQ=255)                                aaGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCa                                                                                                      <  5:270516/139‑1 (MQ=255)                                aaGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCGTATAAGGATTCATGTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCCCCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCa                                                                                                      <  8:162650/139‑1 (MQ=255)                                aaGACATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCa                                                                                                      <  5:131280/139‑1 (MQ=255)                                 aGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCa                                                                                                      >  3:275444/1‑138 (MQ=255)                                  gCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCaaa                                                                                                    >  5:195616/1‑139 (MQ=255)                                  gCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCaaa                                                                                                    >  8:322393/1‑139 (MQ=255)                                         tCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTg                                                                                              <  1:241919/138‑1 (MQ=255)                                           tGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAg                                                                                           >  3:381088/1‑139 (MQ=255)                                            gATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAgc                                                                                          >  5:240255/1‑139 (MQ=255)                                             aTTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAgcg                                                                                         >  8:107981/1‑139 (MQ=255)                                             aTTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGAAACGC‑AATTCAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACTCCATTGCATAATCCTCAAACTCCAGCAgcg                                                                                         >  8:221669/1‑139 (MQ=255)                                              tttCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAgcgc                                                                                        <  5:278605/139‑1 (MQ=255)                                                  aCTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCaaa                                                                                                    <  1:144366/123‑1 (MQ=255)                                                  aCTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCaaa                                                                                                    >  2:144366/1‑123 (MQ=255)                                                                cTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAgc                                                                      >  1:99660/1‑138 (MQ=255)                                                                cTTTCCAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAgc                                                                      >  5:72463/1‑138 (MQ=255)                                                                     cAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCtga                                                                 <  6:240255/139‑1 (MQ=255)                                                                     cAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCtg                                                                  <  7:107981/138‑1 (MQ=255)                                                                          tGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATa                                                                                                             <  6:277956/90‑1 (MQ=255)                                                                          tGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATa                                                                                                             >  5:277956/1‑90 (MQ=255)                                                                                   ttCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATCAGCCGGTCCtt                                                    >  2:21593/1‑138 (MQ=255)                                                                                     cAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATa                                                                                                             >  8:244365/1‑79 (MQ=255)                                                                                     cAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATa                                                                                                             <  7:244365/79‑1 (MQ=255)                                                                                           ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGCGCCGGTCGTTTAAttcttc                                           >  5:14576/1‑139 (MQ=255)                                                                                           ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAAttcttc                                           >  2:23284/1‑139 (MQ=255)                                                                                           ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAAttcttc                                           >  2:383112/1‑139 (MQ=255)                                                                                           ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAAttcttc                                           >  3:175725/1‑139 (MQ=255)                                                                                             ttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATcct                                                                                                        <  3:261673/76‑3 (MQ=255)                                                                                             ttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATcct                                                                                                        >  4:261673/1‑74 (MQ=255)                                                                                             ttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                         >  3:299764/1‑139 (MQ=255)                                                                                              tAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                         <  6:121450/138‑1 (MQ=255)                                                                                              tAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                         <  5:279042/138‑1 (MQ=255)                                                                                              tAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                         <  3:403773/138‑1 (MQ=255)                                                                                                     tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCgg                                                         >  8:329325/1‑115 (MQ=255)                                                                                                     tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCgg                                                         <  7:329325/115‑1 (MQ=255)                                                                                                     tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                  >  7:953/1‑138 (MQ=255)                                                                                                     tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                  >  6:47838/1‑138 (MQ=255)                                                                                                     tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                  >  7:16072/1‑138 (MQ=255)                                                                                                     tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                  >  5:177528/1‑138 (MQ=255)                                                                                                         tGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGAc                               <  8:346371/137‑1 (MQ=255)                                                                                                            tACGCAAAT‑CAGTCACCTCATTATAAACTGAACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAgcg                          <  6:67610/139‑1 (MQ=255)                                                                                                              cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                         <  2:336516/122‑1 (MQ=255)                                                                                                              cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                         >  1:336516/1‑122 (MQ=255)                                                                                                              cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCa                        <  8:953/139‑1 (MQ=255)                                                                                                              cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCa                        <  7:302504/139‑1 (MQ=255)                                                                                                                cAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACt                      >  3:251418/1‑139 (MQ=255)                                                                                                                    atcAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTcc                   <  7:34183/137‑1 (MQ=255)                                                                                                                    atcAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTcc                   <  7:322393/137‑1 (MQ=255)                                                                                                                                aTTTTAAACTGTCCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGGCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGaca         <  7:24754/137‑1 (MQ=255)                                                                                                                                aTTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACAcc       >  1:233020/1‑139 (MQ=255)                                                                                                                                      aaCTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTcac  <  8:16072/138‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTCGTTTGAAGCAAGCTTCACAACTTTATCCAAGCCATATTCTGATTTCACTGCTTCAATCGGCTTTCCAGGCTGATAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCAC  >  CP006881/2189245‑2189515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑