Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,189,447	T→C	T243A (ACT→GCT)	U712_10970 ←	Tryptophan synthase alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,189,447	0	T	C	100.0%	301.9 / NA	92	T243A (ACT→GCT)	U712_10970	Tryptophan synthase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/47);  total (45/47)

CAGGCTGATAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAA  >  CP006881/2189313‑2189578                                                                                                                                       |                                                                                                                                    cAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCtga                                                                                                                                <  6:240255/139‑1 (MQ=255) cAGGCTGATAAGGCTTCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCtg                                                                                                                                 <  7:107981/138‑1 (MQ=255)               ttCAGCTGTTTTAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATCAGCCGGTCCtt                                                                                                                   >  2:21593/1‑138 (MQ=255)                       ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGCGCCGGTCGTTTAAttcttc                                                                                                          >  5:14576/1‑139 (MQ=255)                       ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAAttcttc                                                                                                          >  2:23284/1‑139 (MQ=255)                       ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAAttcttc                                                                                                          >  3:175725/1‑139 (MQ=255)                       ttttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAAttcttc                                                                                                          >  2:383112/1‑139 (MQ=255)                         ttAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        >  3:299764/1‑139 (MQ=255)                          tAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        <  3:403773/138‑1 (MQ=255)                          tAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        <  6:121450/138‑1 (MQ=255)                          tAAATGTTCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        <  5:279042/138‑1 (MQ=255)                                 tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCgg                                                                                                                        <  7:329325/115‑1 (MQ=255)                                 tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCgg                                                                                                                        >  8:329325/1‑115 (MQ=255)                                 tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                                                                                 >  5:177528/1‑138 (MQ=255)                                 tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                                                                                 >  7:953/1‑138 (MQ=255)                                 tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                                                                                 >  7:16072/1‑138 (MQ=255)                                 tCTTTGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCt                                                                                                 >  6:47838/1‑138 (MQ=255)                                     tGATACGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGAc                                                                                              <  8:346371/137‑1 (MQ=255)                                        tACGCAAAT‑CAGTCACCTCATTATAAACTGAACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAgcg                                                                                         <  6:67610/139‑1 (MQ=255)                                          cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        <  2:336516/122‑1 (MQ=255)                                          cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        >  1:336516/1‑122 (MQ=255)                                          cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCa                                                                                       <  7:302504/139‑1 (MQ=255)                                          cGCAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCa                                                                                       <  8:953/139‑1 (MQ=255)                                            cAAAT‑CAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACt                                                                                     >  3:251418/1‑139 (MQ=255)                                                atcAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTcc                                                                                  <  7:322393/137‑1 (MQ=255)                                                atcAGTCACCTCATTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTcc                                                                                  <  7:34183/137‑1 (MQ=255)                                                            aTTTTAAACTGTCCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGGCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGaca                                                                        <  7:24754/137‑1 (MQ=255)                                                            aTTTTAAACTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACAcc                                                                      >  1:233020/1‑139 (MQ=255)                                                                  aaCTGTACAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTcac                                                                 <  8:16072/138‑1 (MQ=255)                                                                       taCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGAc                                                                                              >  8:275433/1‑104 (MQ=255)                                                                       taCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGAc                                                                                              <  7:275433/104‑1 (MQ=255)                                                                       taCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAgcg                                                                                         >  7:385226/1‑109 (MQ=255)                                                                       taCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAgcg                                                                                         <  8:385226/109‑1 (MQ=255)                                                                       taCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCCCTTCCCACTACGACACCGTAACTAAttt                                                           >  5:282044/1‑139 (MQ=255)                                                                       taCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAAttt                                                           >  3:232715/1‑139 (MQ=255)                                                                       taCAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAAttt                                                           >  5:138933/1‑139 (MQ=255)                                                                         cAAGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttca                                                         <  4:275444/139‑1 (MQ=255)                                                                           aGCCGCTAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttcatt                                                       <  2:233020/139‑1 (MQ=255)                                                                            gccgctAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTcc                                                                                  <  8:216459/111‑1 (MQ=255)                                                                            gccgctAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTcc                                                                                  >  7:216459/1‑111 (MQ=255)                                                                            gccgctAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttcattc                                                      <  8:444031/139‑1 (MQ=255)                                                                            gccgctAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttcatt                                                       >  7:444031/1‑138 (MQ=255)                                                                             ccgctAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttcattca                                                     <  1:23284/139‑1 (MQ=255)                                                                             ccgctAAACGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttcattca                                                     <  6:177528/139‑1 (MQ=255)                                                                                ctaaaCGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACTACACCGTCACTAATTTCATTCATCt                                                  >  5:438910/3‑139 (MQ=255)                                                                                 taaaCGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        <  3:206766/83‑1 (MQ=255)                                                                                 taaaCGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTa                                                                                                        >  4:206766/2‑84 (MQ=255)                                                                                 taaaCGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCtt                                                 >  3:397397/2‑139 (MQ=255)                                                                                 taaaCGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCtt                                                 >  1:370998/2‑139 (MQ=255)                                                                                 taaaCGCCATTGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCtt                                                 >  8:404414/2‑139 (MQ=255)                                                                                       ccattGCATAATCCTCACACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGAc                                           <  8:142207/136‑1 (MQ=255)                                                                                       ccattGCATAATCCTCAAACTCCTGCAGCGCCTGTTTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGAc                                           <  7:221669/136‑1 (MQ=255)                                                                                       ccattGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGAc                                           <  7:14382/136‑1 (MQ=255)                                                                                       ccattGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGAc                                           <  6:195616/136‑1 (MQ=255)                                                                                       ccattGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGAc                                           <  4:251418/136‑1 (MQ=255)                                                                                       acattGCATAATGCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGAc                                           <  1:21593/136‑1 (MQ=255)                                                                                          ttGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttcattca                                                     <  4:51854/126‑1 (MQ=255)                                                                                          ttGCATAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATttcattca                                                     >  3:51854/1‑126 (MQ=255)                                                                                              atAATCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTca                                    <  4:232715/138‑2 (MQ=255)                                                                                                aaTCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTcacg                                  <  2:99660/139‑1 (MQ=255)                                                                                                 aTCCTCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTcacg                                  <  6:138933/138‑1 (MQ=255)                                                                                                  tcctCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCTCTGATGAGACGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACAGTCAATAATTTCATTCATCTTTATGACCTGTTCACGGt                                <  3:286029/136‑1 (MQ=255)                                                                                                  tcctCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCTTCACTAATTTCATTCATCTTTATGACCTGTTCACGGt                                >  2:295546/4‑139 (MQ=255)                                                                                                  tcctCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGt                                >  5:402003/4‑139 (MQ=255)                                                                                                  tcctCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGt                                >  7:368776/4‑139 (MQ=255)                                                                                                       aaaCTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGa                            >  1:231930/1‑138 (MQ=255)                                                                                                       aaaCTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTATATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCAGTAATTTCATTAATCTTTATGACCTGTTCACGGTTTGa                            >  1:149230/1‑138 (MQ=255)                                                                                                            ccTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTcacg                                  <  5:368537/127‑1 (MQ=255)                                                                                                            ccTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTcacg                                  >  6:368537/1‑127 (MQ=255)                                                                                                            ccTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATAcc                      >  7:410168/1‑139 (MQ=255)                                                                                                             cTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCATCTATTTTTCAGACGAGCGCACTTCACACTACCACACCGTCACTAATTTAATTCATCTTTATGACCTGTTCACGGGTTGATATAcc                      >  3:164128/1‑138 (MQ=255)                                                                                                              tGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCg                     >  7:71465/1‑138 (MQ=255)                                                                                                              tGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGa                    >  8:95268/1‑139 (MQ=255)                                                                                                              tGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACGAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCg                     >  5:282578/1‑138 (MQ=255)                                                                                                                 aGCGCCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAAcc                 <  4:442194/139‑1 (MQ=255)                                                                                                                   cgcCTGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCGATTTTTCTGACGAGCGCCCTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAAccc                >  7:92306/1‑138 (MQ=255)                                                                                                                     cctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTGTTCTATTTTTATGACGAGCGCCCTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCATGGTTTGATATACCGCAcccta              >  5:56339/3‑136 (MQ=255)                                                                                                                     cctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGt                                >  3:9300/3‑120 (MQ=255)                                                                                                                     cctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGt                                <  4:9300/118‑1 (MQ=255)                                                                                                                     cctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAAcccta              >  8:32619/3‑136 (MQ=255)                                                                                                                     cctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTAc             >  4:387401/3‑139 (MQ=255)                                                                                                                     cctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTATTCTATTTTTATGACGAGCGCACTTCCCACAACGACACCGTCACTAAATTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAAcccta              >  6:2179/3‑136 (MQ=255)                                                                                                                      ctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTACTTCTTCTATTTTTGTGACGAGCGCACTTCGCACTACGACACCGTGACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACa            <  5:292199/138‑1 (MQ=255)                                                                                                                      ctGATTTCTCGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACa            <  3:387401/138‑1 (MQ=255)                                                                                                                            tctcGTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAAc       <  4:397397/138‑1 (MQ=255)                                                                                                                                gtatCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa  <  1:235750/136‑1 (MQ=255)                                                                                                                                gTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa  <  2:370998/139‑1 (MQ=255)                                                                                                                                gTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa  <  8:368776/139‑1 (MQ=255)                                                                                                                                gTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa  <  1:383112/139‑1 (MQ=255)                                                                                                                                gTTTCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa  <  7:95268/139‑1 (MQ=255)                                                                                                                                   tCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCa                                                                                       <  8:157288/51‑1 (MQ=255)                                                                                                                                   tCAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCa                                                                                       >  7:157288/1‑51 (MQ=255)                                                                                                                                       |                                                                                                                                    CAGGCTGATAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAA  >  CP006881/2189313‑2189578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑