| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,189,601 | C→A | R191R (CGG→CGT) | U712_10970 ← | Tryptophan synthase alpha chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,189,601 | 0 | C | A | 97.1% | 210.2 / ‑2.2 | 68 | R191R (CGG→CGT) | U712_10970 | Tryptophan synthase alpha chain |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (32/34); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
CTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAGCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGC > CP006881/2189469‑2189729 | ttcttctATTTTTCTGACGCGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGGTTGATATACCGAACCCTACAGCACCCGGAACAGTGCTGAGATTCTTCACAGTACGAATg < 6:282044/138‑1 (MQ=255) ttctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAAc < 5:47838/139‑1 (MQ=255) ctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTTTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGaa < 6:72463/136‑1 (MQ=255) ctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGaa < 7:404414/136‑1 (MQ=255) ctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAAc < 4:299764/137‑1 (MQ=255) ctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAAc < 6:402003/137‑1 (MQ=255) ttCTGACGAGCGCTCTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAAGAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTaca < 3:334665/138‑1 (MQ=255) aCGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACgg < 8:410168/136‑1 (MQ=255) cACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGTACGGGTACACGGATGAATTGAACTCATTg > 6:120515/1‑139 (MQ=255) gccacCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGaca > 2:198319/3‑139 (MQ=255) gACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGaca > 2:231628/1‑139 (MQ=255) acacCGTCACTAATTTCATTCATCATTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGGACACGGATGAATTGAACTCATAGCGGacac < 1:226515/139‑1 (MQ=255) cacCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAAt > 7:280130/1‑120 (MQ=255) cacCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAAt < 8:280130/120‑1 (MQ=255) cGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCTTTGCGGACACCGGt < 5:2179/139‑1 (MQ=255) cGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGt < 6:438910/139‑1 (MQ=255) cGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGt < 4:175725/139‑1 (MQ=255) tCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCa > 3:408953/1‑123 (MQ=255) tCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCa < 4:408953/123‑1 (MQ=255) tCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTca > 8:121040/1‑139 (MQ=255) tCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCAGAGATTCTTCACAGTACGAATGAACGGGACCACGGATGAATTGACCTCATTGCGGACTCCGGTcc > 6:65256/1‑138 (MQ=255) tCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTca > 5:80128/1‑139 (MQ=255) aTTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTGACAGTACGAATGAACGGGTACACGGATGAATTGTACTCATTGCGGACACCGGTCACAccc < 4:164128/138‑2 (MQ=255) atCTTTATGACCTGTTCATGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa < 6:56339/139‑1 (MQ=255) atCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa < 7:443294/139‑1 (MQ=255) atCTTTATGACCTGTTCACGGTTTGATATAACGAACCCTACAGCAACCGGAACAGTGCTGAGATTGTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACAGGTCACACCCAGAGAAGATa < 1:295546/139‑1 (MQ=255) cTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa > 4:174271/1‑137 (MQ=255) cTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa < 3:174271/137‑1 (MQ=255) cTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATAGCGGACACCGCTCACACCCAGAGAAGATaca > 3:400628/1‑139 (MQ=255) cTGTTCACGGTTTGATATACCCAACCCTACACCCACCGGAACAGTGCCGAGATGCTTCACAGAAGGAATGAACGGGTACACGGATGAATGGCACTCTTTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACg < 6:14576/139‑1 (MQ=255) tGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa < 2:310461/127‑1 (MQ=255) tGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa > 1:310461/1‑127 (MQ=255) acgGTTTGATATACCGAACCCTACAGCAACCGGAAGAGTGCTGAGATTGTTCTCAGTACGATTGAACGGGTACACGTATGAATTGAACTCATTGCGGAGACCGGTCACAGGAAGAGAAGATACACAGTAGACGAAcccc < 7:289158/138‑1 (MQ=255) ggTTTGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGCCACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTc > 7:251090/1‑139 (MQ=255) tttGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTaca > 2:389644/1‑70 (MQ=255) tttGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTaca < 1:389644/70‑1 (MQ=255) ttGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAGCCCTCGGc < 6:80128/139‑1 (MQ=255) tGATATCCCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGc < 7:32619/138‑1 (MQ=255) tGATATACCGAACCCTACAGCAACCGGACCAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGc < 6:282578/138‑1 (MQ=255) tGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGc < 1:231628/138‑1 (MQ=255) tGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGc < 1:198319/138‑1 (MQ=255) tGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGc < 8:71465/138‑1 (MQ=255) tGATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCt > 5:259535/1‑139 (MQ=255) tGATAAACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACCGTACGAATGACCGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGc < 4:381088/138‑1 (MQ=255) gATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTCCACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCtt > 6:227369/1‑139 (MQ=255) gATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCtt > 8:285306/1‑139 (MQ=255) gATATACCGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCtt > 7:126009/1‑139 (MQ=255) cGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATg < 8:315898/50‑1 (MQ=255) cGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATg > 7:315898/1‑50 (MQ=255) cGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACCCCGGTCACACCCAGAGAAGATACACAGTAGACGACCCCCTCGGCTTGTTCaa > 1:160186/1‑138 (MQ=255) cGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCaa > 4:391786/1‑138 (MQ=255) cGAACCCTACAGCAACCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCaa > 1:131987/1‑138 (MQ=255) aCCGGAACAGTGCTGAGATTCTTCACAGTACGAATGCACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCCCACCCAGAGAAGATACACAGTAGACGAACCCCACGGCTAGTTCAATAATGGTTTTCAAAc > 5:152654/1‑139 (MQ=255) aCCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGAt < 8:416962/51‑1 (MQ=255) aCCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGAt > 7:416962/1‑51 (MQ=255) aCCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAgaga > 4:30180/1‑89 (MQ=255) aCCGGAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAgaga < 3:30180/89‑1 (MQ=255) ggAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa > 4:294760/1‑91 (MQ=255) ggAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATa < 3:294760/91‑1 (MQ=255) ggAACAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAACCGGc > 3:150293/1‑139 (MQ=255) aCAGTGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCttt > 6:364196/1‑139 (MQ=255) tGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCt > 7:7724/1‑139 (MQ=255) tGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCt > 2:33223/1‑139 (MQ=255) tGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCt > 4:303512/1‑139 (MQ=255) tGCTGAGATTCTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCt > 4:90471/1‑139 (MQ=255) atcTTCACACTACGCATGAACGGGGACCCGGATGAATTGAACTCATTGCGGACACCGGGCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGGTCAATAATGGTTTTCAAACGGCTTTCGCGTGTCGgcg < 4:235366/137‑1 (MQ=255) cTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAACCGGCTTTCGCTTGTCGgcgc > 5:76160/1‑138 (MQ=255) cTTCACAGTACGAATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGgcgc > 5:33938/1‑138 (MQ=255) | CTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAGCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGC > CP006881/2189469‑2189729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |