Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,189,748 T→C V142V (GTA→GTG)  U712_10970 ← Tryptophan synthase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,189,7480TC99.0% 307.4 / NA 96V142V (GTA→GTG) U712_10970Tryptophan synthase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (45/50);  minor base G (0/1);  total (45/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAGCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTTACCTCATGGTTTTTGCAATCCGCTTGAAGGCTGGCGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATGTGATTTTTCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAACACAGGA  >  CP006881/2189616‑2189880
                                                                                                                                    |                                                                                                                                    
cacGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTc                                                                                                                                 <  8:92306/138‑1 (MQ=255)
 acGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               <  7:285306/139‑1 (MQ=255)
 acGGATGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               <  6:259535/139‑1 (MQ=255)
     aTGAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATggct                                                                                                                           >  2:114206/1‑137 (MQ=255)
       gAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCATTATTGGGTTTCAAACGGCTTTGGCTTGTCGGCGCTCCTAAAGCATTATACGTCTCCTCATGGcttg                                                                                                                         >  2:416212/1‑138 (MQ=255)
       gAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGcttt                                                                                                                         >  3:280436/1‑139 (MQ=255)
       gAATTGAACTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGcttt                                                                                                                         >  2:49695/1‑139 (MQ=255)
             aaCTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               <  5:321970/127‑1 (MQ=255)
             aaCTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               >  6:321970/1‑127 (MQ=255)
             aaCTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACatt                                                                                                                   >  6:126814/1‑137 (MQ=255)
             aaCTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTACACGAACCCCTCTGCTTGTTCAATAATGGTTATCAAACGGCTTTCGCTTGTCGGCTCAACTAAAGAAATATACGTCACCTCATGTCTTTTACat                                                                                                                    >  7:228640/1‑137 (MQ=255)
              aCTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTc                                                                                                                  <  5:364196/139‑1 (MQ=255)
               cTCATTGCGGACACCGGTCACCCCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTACAAACGGCTATCGCATGTCGGCGCAACTAAAGAAATATACGTCACCTCATGCCTTTTACATTcc                                                                                                                 >  3:394411/1‑139 (MQ=255)
               cTCATTGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATTGTTTTCAAACGGCTTTCGCTTGTCGGTGCAACTAAAGAAATATACGTCACCTCATGGCATTTACATTcc                                                                                                                 >  5:227986/1‑139 (MQ=255)
                    tGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTcatctt                                                                                                             >  4:342461/1‑133 (MQ=255)
                    tGCGGACACCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCtt                                                                                                             >  4:374980/1‑138 (MQ=255)
                           acCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTg                                                                                                     <  5:120515/139‑1 (MQ=255)
                           acCGGTCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTg                                                                                                     <  3:374980/139‑1 (MQ=255)
                                tCACACCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTgttgct                                                                                                <  4:280436/139‑6 (MQ=255)
                                 cacaCCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCtt                                                                                               <  2:17270/139‑1 (MQ=255)
                                  acacCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCttt                                                                                              <  1:421212/139‑1 (MQ=255)
                                    acCCAGAGAAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTttct                                                                                            >  4:419154/1‑139 (MQ=255)
                                         gagaAGATACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGCAGGCTGGTGCTTTCTTCTaa                                                                                       >  1:330362/1‑139 (MQ=255)
                                          agaagaTACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATCAACCTCACCTCATGGCTTTTACATTCATCTTGAAGGGTGTTGCTTTCTTCTAAt                                                                                      >  3:51700/1‑139 (MQ=255)
                                             agaTACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGc                                                                                   <  2:131987/139‑1 (MQ=255)
                                             agaTACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGc                                                                                   <  8:7724/139‑1 (MQ=255)
                                              gaTACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATgg                                                                                    >  2:268158/1‑137 (MQ=255)
                                              gaTACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCATTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAGGGCTTTTACATTACTCTTGAAGGCTGTTGCTTTCTTCTAATgg                                                                                    >  5:42311/1‑137 (MQ=255)
                                                tACACAGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATgg                                                                                    >  2:328567/1‑135 (MQ=255)
                                                  cacaGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGAt                                                                               <  8:228640/138‑1 (MQ=255)
                                                  cacaGTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATc                                                                              >  5:297120/1‑139 (MQ=255)
                                                      gTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTACTCTTGAAGGCTGTTGCTTTCTTCTAATTGCAGATCCgg                                                                           >  3:110052/1‑138 (MQ=255)
                                                      gTAGACGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTATCGCTTGTCGGCGCAACTAAAGAAATATACGACACCTCAAGGCTTTTAGATTCCTCTTGAAGGCAGAATCTTACTTCAAATGGCACATCCgg                                                                           >  7:215323/1‑138 (MQ=255)
                                                          aCGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               >  8:252975/1‑82 (MQ=255)
                                                          aCGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               <  7:252975/82‑1 (MQ=255)
                                                          aCGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTg                                                                                                            >  1:208624/1‑101 (MQ=255)
                                                          aCGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTg                                                                                                            <  2:208624/101‑1 (MQ=255)
                                                          aCGAACCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAAc                                                                        >  3:304560/1‑137 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               >  6:197617/2‑77 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               >  6:204308/2‑77 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               <  5:204308/76‑1 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCAt                                                                                                                               <  5:197617/76‑1 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGa                                                                 >  4:181476/2‑139 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGa                                                                 >  3:136060/2‑139 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGa                                                                 <  1:328567/138‑1 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGa                                                                 >  7:286620/2‑139 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGa                                                                 >  7:335828/2‑139 (MQ=255)
                                                               ccccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCAGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGa                                                                 >  7:286488/2‑139 (MQ=255)
                                                               ccacTCGGCTTGTTCAATAAAGGTTTTCAAACGGCATTCGCTTGTCGGCGCAACTAAAGAATTTTACGTCACCTCATAGCTTTAAAATTCGTCTTGAAGGCTGTTGCTTTCTTCTAATGGCATATCCGGAACAAGCAGa                                                                 >  4:221691/4‑139 (MQ=255)
                                                                cccTCGGCTTGATCAATAATGGTTTTCAAACGTCTTTCGCTTGTCGTCGCAACTAAAGATATCTACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGTACAAGCAGAc                                                                <  2:149230/139‑1 (MQ=255)
                                                                 ccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCTCCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGAcc                                                               <  8:286620/139‑1 (MQ=255)
                                                                 ccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGAcc                                                               <  1:401316/139‑1 (MQ=255)
                                                                 ccTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGGTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGccc                                                               >  5:341492/1‑139 (MQ=255)
                                                                   tCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGt                                                             >  4:281816/1‑139 (MQ=255)
                                                                     ggCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCCGCGCAACTAAAGAAAAATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGt                                                             <  1:416212/137‑1 (MQ=255)
                                                                          gTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAAtat                                                       <  3:281816/138‑3 (MQ=255)
                                                                                        ttCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGt                                         >  5:162234/1‑138 (MQ=255)
                                                                                        ttCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGt                                         >  1:31911/1‑138 (MQ=255)
                                                                                         tCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTa                                        <  1:114206/138‑1 (MQ=255)
                                                                                               ggCTATCGCTTGTCGGCGCAACTACAGAAATATACGTCACCTCCTGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGaa                                 <  8:251090/139‑1 (MQ=255)
                                                                                                    tCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCgg                                                                           <  6:443265/92‑1 (MQ=255)
                                                                                                    tCGCTTGTCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCgg                                                                           >  5:443265/1‑92 (MQ=255)
                                                                                                           tCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGc                                                                                   <  8:394082/77‑1 (MQ=255)
                                                                                                           tCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGc                                                                                   >  7:394082/1‑77 (MQ=255)
                                                                                                           tCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGt                            <  3:365873/132‑1 (MQ=255)
                                                                                                           tCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGt                            >  4:365873/1‑132 (MQ=255)
                                                                                                           tCGGCGCAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCttt                     >  1:231157/1‑139 (MQ=255)
                                                                                                               cgcAACTAAAGAACTATACGTGACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTc                 <  5:65256/139‑1 (MQ=255)
                                                                                                               cgcAACTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTc                 <  7:121040/139‑1 (MQ=255)
                                                                                                               cgcAACTAAAGAAATATACGTCACCACATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTc                 <  5:264699/139‑1 (MQ=255)
                                                                                                                  aaCTAAAGTAATATACGTCACCTCATTGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTATAATGGCAGATCCGGAACAAGCAGACCGTCAATCTGATTTTCCCGCAGTTAAGCGAAAAAGTATTCTTTGTTCAAt              <  6:42311/139‑1 (MQ=255)
                                                                                                                  aaCTAAAGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAAt              <  5:126814/139‑1 (MQ=255)
                                                                                                                        aGAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTaa         >  4:8583/1‑138 (MQ=255)
                                                                                                                         gAAATATACGTCACGTCATGGCTTTTACATTCCTCTTGTAGGCTGTTGCTTTGTTCTAATGGCAGATCCGGATCAAGCAGACCGTCAATATGATTTTGCCGCTGTAATGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  5:129385/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  1:268158/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  4:150293/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  4:136060/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  4:110052/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  3:342461/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  4:304560/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  6:33938/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  5:227369/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  5:233864/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  6:193147/139‑1 (MQ=255)
                                                                                                                         gAAATATACGTCACCTCATGGCCTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTCAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  6:76160/139‑1 (MQ=255)
                                                                                                                          aaaTATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAacac      <  6:297120/139‑1 (MQ=255)
                                                                                                                           aaTATACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAAcaca     <  6:162234/139‑1 (MQ=255)
                                                                                                                           aaTATACGTCACCTCATGGCTTTTACAATCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAAcaca     <  2:414863/139‑1 (MQ=255)
                                                                                                                            atatACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCaa               <  6:29423/128‑1 (MQ=255)
                                                                                                                            atatACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCaa               >  5:29423/1‑128 (MQ=255)
                                                                                                                            atatACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAACACAg    >  2:142995/1‑139 (MQ=255)
                                                                                                                            atatACGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAACACAg    >  6:215620/1‑139 (MQ=255)
                                                                                                                             tataCGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAACACAg    >  8:60419/1‑138 (MQ=255)
                                                                                                                              ataCGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       <  6:270828/134‑1 (MQ=255)
                                                                                                                              ataCGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAaca       >  5:270828/1‑134 (MQ=255)
                                                                                                                               taCGTCACCTCATGGCTTTTACATTCCTCTTGAAGGCTGTTGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATATGATTTTCCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAACACAGGa  <  3:391786/138‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
CACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAGCCCTCGGCTTGTTCAATAATGGTTTTCAAACGGCTTTCGCTTGTCGGCGCAACTAAAGAAATATACGTTACCTCATGGTTTTTGCAATCCGCTTGAAGGCTGGCGCTTTCTTCTAATGGCAGATCCGGAACAAGCAGACCGTCAATGTGATTTTTCCGCAGTAAAGCGAAAAAGTATTCTTTGTTCAATTGTAACACAGGA  >  CP006881/2189616‑2189880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑