Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,190,027 T→A S49S (TCA→TCT)  U712_10970 ← Tryptophan synthase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,190,0270TA100.0% 143.7 / NA 46S49S (TCA→TCT) U712_10970Tryptophan synthase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/25);  total (21/25)

CGTAAAGAGGATAATCGGAATATTCACTCCGTTTTTTTTCATTTCCCCGCCTAATTCGATTGCCTTTACGATATTCATTCCATGATCAAGCGCCCGCTTTGAAGCACGCTGGATCACCGGACCGTCTGCAAGCGGGTCTGAGTATGCAACACCAAGCTCCAGTGCCGTGGCACCTGCTTTTTGGAGTGACTTCGCCAGTTTAACCGAAACCTCAGGCGATGGATCTCCCGCTGTAATAAACGGGATAAACAATTTTTCTGATGCTTGAAGATC  >  CP006881/2189889‑2190161
                                                                                                                                          |                                                                                                                                      
cGTAAAGAGGATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCCTGTCt                                                                                                                                        >  5:89745/1‑139 (MQ=255)
  tAAAGAGGATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGa                                                                                                                                      <  5:215620/139‑1 (MQ=255)
  tAAAGAGGATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGa                                                                                                                                      <  8:272006/139‑1 (MQ=255)
     agagGATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGa                                                                                                                                      <  5:89031/136‑1 (MQ=255)
     agagGATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGa                                                                                                                                      >  6:89031/1‑136 (MQ=255)
         gATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTGCTTCTTCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCAGCAAGCGGGTCAGAGTATGc                                                                                                                                >  1:13668/1‑138 (MQ=255)
         gATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGCCCGTCTGCACGCGGGTCAGAGTATGc                                                                                                                                >  2:344949/1‑138 (MQ=255)
         gATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCa                                                                                                                               <  7:121533/139‑1 (MQ=255)
         gATAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCa                                                                                                                               <  8:335828/139‑1 (MQ=255)
          aTAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGGaa                                                                                                                              >  8:95301/1‑139 (MQ=255)
          aTAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCaa                                                                                                                              <  2:309728/139‑1 (MQ=255)
          aTAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCaa                                                                                                                              >  1:309728/1‑139 (MQ=255)
          aTAATCGGAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACAGGACCGTCTGCAAGCGGGTCAGAGTATGCaa                                                                                                                              >  6:47/1‑139 (MQ=255)
                ggAATATTCACTGCGATTTTTTTCATTTCTCCGCCTAATTCGAGTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCTAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  1:351704/138‑1 (MQ=255)
                ggAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  7:313427/138‑1 (MQ=255)
                ggAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  6:313878/138‑1 (MQ=255)
                ggAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  5:212040/138‑1 (MQ=255)
                ggAATATTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACAGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  3:375224/138‑1 (MQ=255)
                    tatTCACTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTc                                                                                                                    <  4:324728/139‑1 (MQ=255)
                         aCTCCGTTTTTTTTCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATg                                                                                                               >  8:119149/1‑139 (MQ=255)
                                   ttttCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGcgc                                                                                                      <  8:240302/138‑3 (MQ=255)
                                     ttCATTTCTCCGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCt                                                                                                    <  1:175340/138‑1 (MQ=255)
                                               cGCCTAATTCGATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGa                                                                                          <  4:300219/138‑1 (MQ=255)
                                                         gATTGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAACCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGc                                                                                <  2:13668/138‑1 (MQ=255)
                                                           ttGCCTTTACGATATTCATTCCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCACGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAg                                                                             >  1:339408/1‑139 (MQ=255)
                                                                         ttcattcCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGa                                                                                          >  5:343843/1‑112 (MQ=255)
                                                                         ttcattcCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGa                                                                                          <  6:343843/112‑1 (MQ=255)
                                                                         ttcattcCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGAAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCt                                                               >  2:279375/1‑139 (MQ=255)
                                                                           cattcCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTc                                                              <  5:155429/138‑1 (MQ=255)
                                                                                cttGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAggaa                                                         <  2:330362/136‑3 (MQ=255)
                                                                                 ttGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAggaact                                                       >  5:239721/2‑135 (MQ=255)
                                                                                   gATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAggaactg                                                      >  1:81388/1‑133 (MQ=255)
                                                                                        aGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         >  2:241969/1‑66 (MQ=255)
                                                                                        aGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  8:289157/66‑1 (MQ=255)
                                                                                        aGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  1:241969/66‑1 (MQ=255)
                                                                                        aGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         >  7:289157/1‑66 (MQ=255)
                                                                                           gcCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCg                                             <  7:119149/139‑1 (MQ=255)
                                                                                           gcCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCg                                             <  2:81388/139‑1 (MQ=255)
                                                                                              cGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCCACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGccg                                          >  4:169507/1‑137 (MQ=255)
                                                                                                cTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         >  7:13306/1‑58 (MQ=255)
                                                                                                cTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCa                                                                                                                         <  8:13306/58‑1 (MQ=255)
                                                                                                                    ccGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAAttt                    >  4:254598/1‑139 (MQ=255)
                                                                                                                         ccGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGGAATAAACGGGATAAACAATTTTTCt                >  3:433784/1‑138 (MQ=255)
                                                                                                                            tCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTAAAACGAAACCTCAGGAACGGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATg            >  6:2237/1‑139 (MQ=255)
                                                                                                                                      ggTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGCTCGCCCGCCGTAATAAACGGGATAAAAAATTTTTCTGATGGTTGAAGATc  >  3:352995/1‑139 (MQ=255)
                                                                                                                                      ggTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATc  <  7:95301/139‑1 (MQ=255)
                                                                                                                                      ggTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTGTTTGGAGTGACTTCGCCACTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAAATTTTCTGATGGTTGAAGATc  >  1:267018/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                      
CGTAAAGAGGATAATCGGAATATTCACTCCGTTTTTTTTCATTTCCCCGCCTAATTCGATTGCCTTTACGATATTCATTCCATGATCAAGCGCCCGCTTTGAAGCACGCTGGATCACCGGACCGTCTGCAAGCGGGTCTGAGTATGCAACACCAAGCTCCAGTGCCGTGGCACCTGCTTTTTGGAGTGACTTCGCCAGTTTAACCGAAACCTCAGGCGATGGATCTCCCGCTGTAATAAACGGGATAAACAATTTTTCTGATGCTTGAAGATC  >  CP006881/2189889‑2190161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑