Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,173,301 A→G L149L (CTT→CTC)  U712_10870 ← Bifunctional protein BirA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,173,3010AG97.6% 123.7 / ‑4.6 41L149L (CTT→CTC) U712_10870Bifunctional protein BirA
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (15/25);  total (15/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

AGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGC  >  CP006881/2173168‑2173429
                                                                                                                                     |                                                                                                                                
ggATACCGACTGTTTTTTTTCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCg                                                                                                                              <  8:170068/137‑1 (MQ=255)
            ttttttttCCGTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTTGAGAGGAATGTCAGGccc                                                                                                                 >  4:154557/1‑138 (MQ=255)
              ttttttCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGc                                                                                                                <  8:255523/138‑1 (MQ=255)
                      gTTAATCAAAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAgg                                                                                                       <  5:127712/139‑1 (MQ=255)
                         aatcaaAATATCATTTGGCCATTTAATATCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCt                                                                                                     <  4:212650/138‑1 (MQ=255)
                                                  atatCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTga                                                                            <  8:311902/138‑1 (MQ=255)
                                                  atatCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTga                                                                            <  3:9052/138‑1 (MQ=255)
                                                  atatCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTga                                                                            <  6:207600/138‑1 (MQ=255)
                                                   cagccGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTgaga                                                                          <  7:297011/136‑1 (MQ=255)
                                                     tCCGTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCCACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGaa                                                                         <  3:262231/138‑1 (MQ=255)
                                                        gTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc                                                                     <  5:302749/139‑1 (MQ=255)
                                                        gTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc                                                                     <  7:420153/139‑1 (MQ=255)
                                                        gTTTGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc                                                                     <  8:148667/139‑1 (MQ=255)
                                                         tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACATCAGCTACTGCAGCAAGCAGAGTCAGATGCGGTGTTTTTTGGAGCGGAATGTGAGGCCGCAAAATCAGGCTCATCCCAACACCGTTTCCTTCTTGAGAATGGCa                                                                    >  2:246915/1‑139 (MQ=255)
                                                         tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa                                                                    <  7:309226/139‑1 (MQ=255)
                                                         tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa                                                                    <  6:279196/139‑1 (MQ=255)
                                                         tttGGATGCCTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa                                                                    <  7:19510/139‑1 (MQ=255)
                                                                 ccTGCTGCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCAACCAAACCCCCTTTCCTTCTTGAGAATGCCATAcccctg                                                            >  8:297011/1‑136 (MQ=255)
                                                                  ctgctgCCTCTTCTATTCCCTGCACCACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATAcccctgt                                                           <  1:152074/139‑5 (MQ=255)
                                                                  ctgctgCCTCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCGCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGa                                                           <  3:413945/139‑1 (MQ=255)
                                                                                    ccTGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATTCCATACCCTAGACATTCGGCCCCTTCCGGc                                         >  1:58054/1‑139 (MQ=255)
                                                                                      tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCCAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATTCCATACCCTAGACATTCGGCCCCTGCCCGcgg                                       >  6:9032/1‑137 (MQ=255)
                                                                                      tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCCTACAAACACCGTTTCCTTCTTGAGCATGCCATACCCTAGCCATTAGGCCCCGTCCGGCtg                                       >  4:146728/1‑139 (MQ=255)
                                                                                      tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCtg                                       >  7:50631/1‑139 (MQ=255)
                                                                                      tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCtg                                       >  7:44210/1‑139 (MQ=255)
                                                                                      tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCGTCCGGcgg                                       >  1:106015/1‑137 (MQ=255)
                                                                                      tGCACAACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATAAGGCTCATCCAAACCCCGTTTCCTTGTTGAGAATGCCATACCCGAGACATTCGGCCCCGTCCGGcgg                                       >  1:370288/1‑137 (MQ=255)
                                                                                        cacaACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc                                                                     <  6:199250/107‑1 (MQ=255)
                                                                                        cacaACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGcc                                                                     >  5:199250/1‑107 (MQ=255)
                                                                                        cacaACAGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCtgtt                                     >  7:50230/1‑139 (MQ=255)
                                                                                              aGCTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGtttgtttg                               <  7:192959/139‑1 (MQ=255)
                                                                                                cTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa                                                                    >  1:121496/1‑100 (MQ=255)
                                                                                                cTACTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCa                                                                    <  2:121496/100‑1 (MQ=255)
                                                                                                   cTGCAGGAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGGCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGc                          <  2:283486/139‑1 (MQ=255)
                                                                                                   cTGCAGCAAGCAGAGTCAGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGATTATGCAATACCCTAGACATTCGGCCCCTTCCGGCTgtttttttttattg                          <  1:177909/139‑7 (MQ=255)
                                                                                                                  tcaGCTGCGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGcc           <  8:50631/137‑1 (MQ=255)
                                                                                                                         cGGTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCg      <  8:50230/137‑1 (MQ=255)
                                                                                                                         cGGTGTTATTTGGCGCGGAATGACAGGCCGCAAAATCAGGCTCCTCCAACCACCTTTTCCTTCTTGACATTGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCg      <  8:437665/137‑1 (MQ=255)
                                                                                                                          ggTGTTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCGGTTTTTTTGTAAGCCACCACAAGGGTGCCTTCCgggg   >  4:89143/1‑137 (MQ=255)
                                                                                                                           gtgtTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGATAATTCCATACCCTAGACATTCGGCCCCTTCCGGCTGGTTGTTTGTAAGCCACCACAAGGGTGCCTTCCGGTGc  >  3:100291/1‑139 (MQ=255)
                                                                                                                           gtgtTTTTTGGAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAACACCGTTTCCTTCTTGAGAATGCCATACCCTAGACATTCGGCCCCTTCCGGCTGTTTGTTTGTCAGCCACCACAAGGGTGCCTTCCGGTGc  <  3:89143/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
AGATGCCGACTGTTTTTTTTCCGTTAATTAAAATATCATTAGGCCATTTAATATCCGTTTGGATGCCTGCTGCCGCTTCTATTCCCTGCACAACAGCTACTGCAGCAAGTAATGTGAGCTGCGGTGTTTTTTGAAGCGGAATGTCAGGCCGCAAAATCAGGCTCATCCAAATGCCATTTCCCTCTTGAGAATGCCATACCCTAGACATTCGACCTCTGCCGGCTGTTTGTTTGTCAGCCACCACAAGAGTGCCTTCCGGTGC  >  CP006881/2173168‑2173429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑