Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,190,092 C→T V28I (GTT→ATT)  U712_10970 ← Tryptophan synthase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,190,0920CT98.6% 217.2 / NA 71V28I (GTT→ATT) U712_10970Tryptophan synthase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (40/29);  minor base G (1/0);  total (42/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCATTCCATGATCAAGCGCCCGCTTTGAAGCACGCTGGATCACCGGACCGTCTGCAAGCGGGTCTGAGTATGCAACACCAAGCTCCAGTGCCGTGGCACCTGCTTTTTGGAGTGACTTCGCCAGTTTAACCGAAACCTCAGGCGATGGATCTCCCGCTGTAATAAACGGGATAAACAATTTTTCTGATGCTTGAAGATCTAATTTAAACATGCGCTTTCACCTCTTCTTCCAATACATTCATTAATGTGTTGA  >  CP006881/2189962‑2190215
                                                                                                                                  |                                                                                                                           
ttcattcCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGAAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCt                                                                                                                     >  2:279375/1‑139 (MQ=255)
  cattcCTTGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTc                                                                                                                    <  5:155429/138‑1 (MQ=255)
       cttGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAggaa                                                                                                               <  2:330362/136‑3 (MQ=255)
        ttGATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAggaact                                                                                                             >  5:239721/2‑135 (MQ=255)
          gATCAAGCGCCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAggaactg                                                                                                            >  1:81388/1‑133 (MQ=255)
                  gcCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCg                                                                                                   <  7:119149/139‑1 (MQ=255)
                  gcCCGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCg                                                                                                   <  2:81388/139‑1 (MQ=255)
                     cGCTTTGAAGCCCGCTGGATCACCGGACCGTCTGCAAGCGGGTCAGAGTATGCCACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGccg                                                                                                >  4:169507/1‑137 (MQ=255)
                                           ccGGACCGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAAttt                                                                          >  4:254598/1‑139 (MQ=255)
                                                ccGTCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGGAATAAACGGGATAAACAATTTTTCt                                                                      >  3:433784/1‑138 (MQ=255)
                                                   tCTGCAAGCGGGTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTAAAACGAAACCTCAGGAACGGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATg                                                                  >  6:2237/1‑139 (MQ=255)
                                                             gggcagAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATc                                                        <  5:47/134‑1 (MQ=255)
                                                             ggTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGCTCGCCCGCCGTAATAAACGGGATAAAAAATTTTTCTGATGGTTGAAGATc                                                        >  3:352995/1‑139 (MQ=255)
                                                             ggTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATc                                                        <  7:95301/139‑1 (MQ=255)
                                                             ggTCAGAGTATGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTGTTTGGAGTGACTTCGCCACTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAAATTTTCTGATGGTTGAAGATc                                                        >  1:267018/1‑139 (MQ=255)
                                                                     tctGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGTAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTGGAAGATCCAATTTaa                                                <  7:342580/137‑1 (MQ=255)
                                                                       tGCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTaaa                                               >  8:339300/1‑138 (MQ=255)
                                                                        gCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACa                                             >  2:77017/1‑139 (MQ=255)
                                                                        gCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACa                                             >  6:307957/1‑139 (MQ=255)
                                                                        gCAACACCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATAGAAACCACAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACa                                             >  4:303361/1‑139 (MQ=255)
                                                                          aaCACCAAGCTCCAATGCTGTGGCGCCTGGTTTTTGGTGTGGCTTCGCCAGTTCAATCGAAACCGCAGGGACTGGATCGCCCGCCCGAATAAACGGGATAAACAATTTTTATGATGGTTGTAGCTCCAATTTAAAAATg                                           >  6:64472/1‑139 (MQ=255)
                                                                           acacCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATgg                                          <  2:74632/139‑2 (MQ=255)
                                                                            cacCAAGCTCCAATGCTGTGGCGCCGGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCATGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGGTGGTTGAAGATCCAATTTTAAAATggg                                         >  6:308555/1‑137 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCTCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATgggc                                        >  7:330025/1‑139 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAATCGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATgggc                                        >  8:37063/1‑139 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATgggc                                        >  1:12718/1‑139 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATgggc                                        >  4:94492/1‑139 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATgggc                                        >  5:117263/1‑139 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATgggc                                        >  8:155270/1‑139 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCAGATGGTTGAAGATCCAATTTAAACATgggc                                        >  1:221030/1‑139 (MQ=255)
                                                                             acCAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACATCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAAAAATTTTTCTGATGGGTGAAGATCCAATTTAAACATgggc                                        >  4:191868/1‑139 (MQ=255)
                                                                               cAAGCTCCAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCCCCACTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATTAACAATTTTTCTGATGGTTGAAGATACAATTTAAACATGGGCtt                                      >  4:356902/1‑139 (MQ=255)
                                                                                     ccAATGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACct                                <  1:441647/139‑1 (MQ=255)
                                                                                       aatGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATaaa                                                                                <  7:15640/87‑1 (MQ=255)
                                                                                       aatGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATaaa                                                                                >  8:15640/3‑89 (MQ=255)
                                                                                       aatGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACctc                               >  3:253133/3‑138 (MQ=255)
                                                                                       aatGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACctc                               <  4:204930/136‑1 (MQ=255)
                                                                                       aatGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACctc                               >  3:204930/3‑138 (MQ=255)
                                                                                       aatGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACctc                               <  4:253133/136‑1 (MQ=255)
                                                                                        atGCTGTGGTGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCtctt                             >  8:216121/2‑139 (MQ=255)
                                                                                         tGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCtcttc                            <  6:239721/139‑1 (MQ=255)
                                                                                         tGCTGTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCtcttc                            >  5:146989/1‑139 (MQ=255)
                                                                                             gTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACAt                                            <  7:84504/119‑1 (MQ=255)
                                                                                             gTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACAt                                            >  8:84504/1‑119 (MQ=255)
                                                                                             gTGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTcc                        >  1:233334/1‑139 (MQ=255)
                                                                                             gTGGCGCCTGCTTTTTGGAGTGACTTAGCCAGTTCAATCGAAACCTCAGGCACAGGATCGCCCCCCGTAATAAACGGGCTAAACAAATTTTCTGATGGTTGAATATCCAATTTAAACATGGGCTTTCACCTCttcttc                         >  1:435790/1‑138 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCTCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTcc                        >  1:13439/1‑138 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGa                                                                    >  5:320151/1‑94 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGa                                                                    >  1:364728/1‑94 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGa                                                                    <  2:364728/94‑1 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGa                                                                    <  6:320151/94‑1 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACAt                                            <  2:282441/118‑1 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACAt                                            >  1:282441/1‑118 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTcc                        >  3:439402/1‑138 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTcc                        >  3:210936/1‑138 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCa                       <  3:303361/139‑1 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCa                       <  3:254598/139‑1 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCACAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAAAATGGGCTTTCACCTCTTCTTcc                        >  1:256668/1‑138 (MQ=255)
                                                                                              tGGCGCCTGCTTTTTGGAGTGACTTCGCCAGTTCAAGCGAACCCTCCGGAACTTGATCGCCAGCCGTTATAAACGGGATAAACAAATGTTCTGATGGTTGAAGATCCTATTTCAACATGGGCTTTCAGCTCttcgtcg                        >  4:226745/1‑137 (MQ=255)
                                                                                                  gccTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATAc                   <  6:307171/138‑1 (MQ=255)
                                                                                                  gccTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATAc                   <  4:210936/138‑1 (MQ=255)
                                                                                                  gccTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATAc                   <  6:117263/138‑1 (MQ=255)
                                                                                                  gccTGCTTTTTGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATAc                   <  5:64472/138‑1 (MQ=255)
                                                                                                        tttttGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCAGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCAAATTTAAACATGTGCTTTCACCTCTTATTCCAATAaattcat             >  7:214534/1‑139 (MQ=255)
                                                                                                           ttGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATACATTCATTa           <  5:307957/138‑1 (MQ=255)
                                                                                                           ttGGAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATACATTCATTa           <  1:344949/138‑1 (MQ=255)
                                                                                                             ggAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATACATTCATTAAtg        <  8:352321/139‑1 (MQ=255)
                                                                                                             ggAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATACATTCATTAAt         <  1:77017/138‑1 (MQ=255)
                                                                                                             ggAGTGACTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATACATTCATTAAt         <  7:339300/138‑1 (MQ=255)
                                                                                                             ggAGTGACTTCGCCAGGTCAATCGAAACCTCAGGAACTGGATCGCCCTCCGTAATAAAAGTGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGTCTTTCACCTCTTCTTCCAATACATTCATTAAt         <  7:189342/138‑1 (MQ=255)
                                                                                                                   aCTTCGCCAGTTCAATCGAAACCTCAGGAACTGGATCGCCCGCCGTAATAAACGGGATAAACAATTTTTCTGATGGTTGAAGATCCAATTTAAACATGGGCTTTCACCTCTTCTTCCAATACATTCATTAATGTGTTGa  >  3:99989/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                           
TTCATTCCATGATCAAGCGCCCGCTTTGAAGCACGCTGGATCACCGGACCGTCTGCAAGCGGGTCTGAGTATGCAACACCAAGCTCCAGTGCCGTGGCACCTGCTTTTTGGAGTGACTTCGCCAGTTTAACCGAAACCTCAGGCGATGGATCTCCCGCTGTAATAAACGGGATAAACAATTTTTCTGATGCTTGAAGATCTAATTTAAACATGCGCTTTCACCTCTTCTTCCAATACATTCATTAATGTGTTGA  >  CP006881/2189962‑2190215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑