Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,190,349 G→A L340L (CTC→CTT)  U712_10975 ← Tryptophan synthase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,190,3490GA97.4% 227.8 / ‑3.2 79L340L (CTC→CTT) U712_10975Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (31/44);  total (32/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TGACATCCTTATCTCCCCGTCCTGATAAACAGACGAGAATGGATTGATTGCGATCCATCTCTTTGGCGAGTTTGAATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAAT  >  CP006881/2190213‑2190483
                                                                                                                                        |                                                                                                                                      
tGACATCCTTGTCTCCCCGGCCTGATAAACAGATGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAaaag                                                                                                                                      >  6:167694/1‑139 (MQ=255)
    aTCCTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGctt                                                                                                                                   >  2:250980/1‑138 (MQ=255)
      ccttgcctcCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTaa                                                                                                                                <  6:293531/133‑1 (MQ=255)
      ccTTGTATCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTaa                                                                                                                                <  4:10770/139‑1 (MQ=255)
       cTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAaaagct                                                                                                                                    >  1:273189/1‑132 (MQ=255)
       cTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAaaagct                                                                                                                                    <  2:273189/134‑3 (MQ=255)
       cTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGcttt                                                                                                                                  >  2:398565/1‑136 (MQ=255)
       cTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGcttt                                                                                                                                  <  1:398565/136‑1 (MQ=255)
       cTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAAt                                                                                                                               <  4:249007/139‑1 (MQ=255)
       cTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAAt                                                                                                                               >  5:128637/1‑139 (MQ=255)
       cTTGTCTCCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAAt                                                                                                                               >  1:261321/1‑139 (MQ=255)
         tgtctcCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATg                                                                                                                              >  7:384481/3‑138 (MQ=255)
         tgtctcCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATg                                                                                                                              >  6:439466/3‑138 (MQ=255)
         tgtctcCCCGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATg                                                                                                                              >  7:234767/3‑138 (MQ=255)
              ccccGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCa                                                                                                                        >  7:129771/1‑139 (MQ=255)
               cccGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTTGCGAGTTTGAATGCTTTCGCTAACGCATGGACAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCAc                                                                                                                       <  3:288316/139‑1 (MQ=255)
               cccGGCCTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCAc                                                                                                                       >  4:436588/1‑139 (MQ=255)
                  ggccTGATAACCAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGc                                                                                                                    <  6:31334/137‑1 (MQ=255)
                  ggccTGATAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGc                                                                                                                    <  2:13439/137‑1 (MQ=255)
                        aTAAACAGACGATAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  3:191868/139‑1 (MQ=255)
                        aTAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGcttctt                                                                                                               >  5:106567/1‑138 (MQ=255)
                        aTAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  7:37063/139‑1 (MQ=255)
                        aTAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  8:129771/139‑1 (MQ=255)
                        aTAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  1:250980/139‑1 (MQ=255)
                        aTAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  2:12718/139‑1 (MQ=255)
                         tAAACAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  8:234767/138‑1 (MQ=255)
                          aaaCAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCAt                                                                                                            <  8:50439/139‑1 (MQ=255)
                           caCAGACGAGAATGAGTTGACCGCGATCGATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCAt                                                                                                            <  4:433784/137‑1 (MQ=255)
                           atcAGACGAGCATGTGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAATATCCCCTCTTTTTCTGACAATAGGTTTAATGCATCCACCGCTTCTTCAt                                                                                                            <  6:75282/136‑1 (MQ=255)
                           aaCAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCAt                                                                                                            <  5:439466/138‑1 (MQ=255)
                           aaCAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCAt                                                                                                            <  5:308555/138‑1 (MQ=255)
                             cAGACGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATcgg                                                                                                         >  5:194427/1‑137 (MQ=255)
                              agaatagaATGAGTTGACCGCGATCCATTCCTTAGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTATTCTGACAAAAGCATTAATGCATCCACCGCATCTTCATCggt                                                                                                        >  5:75282/6‑139 (MQ=255)
                                aCGAGAATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCggtta                                                                                                      >  1:430335/1‑137 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATTCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  3:436588/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              >  5:57995/1‑129 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  6:57995/129‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              >  7:59884/1‑129 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCttcttc                                                                                                              <  8:59884/129‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  3:11168/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  2:256668/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  4:439402/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  3:847/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  5:358636/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  2:430335/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  3:434367/139‑1 (MQ=255)
                                  gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTATACTGCATCCACCGCTTCTTCATcggatat                                                                                                     >  4:847/1‑132 (MQ=255)
                                              accgCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCAt                                                                                                            <  4:229669/116‑1 (MQ=255)
                                              accgCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCAt                                                                                                            >  3:229669/4‑119 (MQ=255)
                                              accgCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATaa                                                                                           >  1:12722/4‑136 (MQ=255)
                                              accgCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGAAACCACCGCTTCTTCATCGGGAATACTGTCATAAGt                                                                                         >  3:49478/4‑138 (MQ=255)
                                                    aTCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACgg                                                                                  >  5:269017/1‑139 (MQ=255)
                                                     tCCATTCCTTTGGCGAGTTTTAATGCTTTCGATAACGAATGGGCAGATTCGATTGCCGTCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCAACCACCGCTTCTTCATCGGTTATACTGTAATAAGTGACACGGc                                                                                 <  6:269017/139‑1 (MQ=255)
                                                          tcctTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCAc                                                                                                                       >  2:352953/3‑96 (MQ=255)
                                                          tcctTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCAc                                                                                                                       <  1:352953/94‑1 (MQ=255)
                                                               tGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATg                                                                       >  5:305967/1‑139 (MQ=255)
                                                                ggCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGAAAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATg                                                                       <  2:40423/138‑1 (MQ=255)
                                                                 gCGAGTTTGAATGCTTTCGCTACCGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGCCAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCa                                                                     <  4:132443/139‑1 (MQ=255)
                                                                 gCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGc                                                                      >  1:306405/1‑138 (MQ=255)
                                                                 gCGAGGTTGAATGCGTTCGCTAACGCTTGGGCAGATGCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATCCATCCACCGCAGCTTCATCGGTTCTACTGTCATAAGTGACACGGCCGCTTTTATGCa                                                                     <  3:226745/139‑1 (MQ=255)
                                                                     gTTTGAATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAata                                                                 >  8:122311/1‑139 (MQ=255)
                                                                      tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    >  6:29430/1‑103 (MQ=255)
                                                                      tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                    <  5:29430/103‑1 (MQ=255)
                                                                      tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTTTGCAAaaat                                                                >  1:140144/1‑139 (MQ=255)
                                                                      tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATTGAAataa                                                                >  6:269143/1‑138 (MQ=255)
                                                                           aTGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATTCAAATATGCATg                                                           >  7:274911/1‑139 (MQ=255)
                                                                                         gCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTc                                             <  7:122311/139‑1 (MQ=255)
                                                                                             gggCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCTGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATTCATTGTCCGGACCGATTCGAgg                                         >  3:280094/1‑139 (MQ=255)
                                                                                             gggCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATAATGAAGCGCCGGACCGATTCCAg                                          >  5:146348/1‑138 (MQ=255)
                                                                                                   aTTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAAt                                                                                                                               <  4:190045/47‑1 (MQ=255)
                                                                                                   aTTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAAt                                                                                                                               >  3:190045/1‑47 (MQ=255)
                                                                                                       gATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga                               <  5:167694/139‑2 (MQ=255)
                                                                                                           ggcGGCAAAATCGCGTCTTTTTCTGACATAAGCTTTAATGAATCAACCGCTTCTTCATCGGTTATAGTGTAATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAcc                             <  1:205436/135‑1 (MQ=255)
                                                                                                            ccgcaaaaaGCCCCTCTTTTTCTGAAAAATGTTTTATAGCATCCCCCGGTTCTTCATCGGTTTTACTGTCATAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggc                          <  5:52761/134‑1 (MQ=255)
                                                                                                            ccGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg                           <  3:169507/138‑3 (MQ=255)
                                                                                                              ggCAAAATCCCCTCTTTTTCTGACAACAGCTTTAATCCATCCACCGCTTCTTCATCGGTAATACTGTCATCAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg                        <  8:57178/139‑1 (MQ=255)
                                                                                                              ggCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg                        <  2:306405/139‑1 (MQ=255)
                                                                                                                 aaaaTCCCCTCTTTTTCTTACAAATCCTTTAATGCAACCCCCGCTTCTTCATGGTTTATACTGTCATACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa                     <  5:269143/139‑1 (MQ=255)
                                                                                                                 aaaaTCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa                     <  2:12722/139‑1 (MQ=255)
                                                                                                                        ccTCTTTTTCTGACAAAACCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa              <  3:210219/139‑1 (MQ=255)
                                                                                                                        ccTCTTTATCTCACAAAAGCTTTATTGCCCCCACAGCTTATCCTTCGGTTAACCGGCCAAAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa              <  4:99989/139‑1 (MQ=255)
                                                                                                                                    aCAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGCCGAGACCGGCTGAAATAGAGTAGGGCTCaataat  >  3:218900/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGACATCCTTATCTCCCCGTCCTGATAAACAGACGAGAATGGATTGATTGCGATCCATCTCTTTGGCGAGTTTGAATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAAT  >  CP006881/2190213‑2190483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑