Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,190,382 G→T I329I (ATC→ATA)  U712_10975 ← Tryptophan synthase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,190,3820GT90.3% 72.5 / ‑3.8 33I329I (ATC→ATA) U712_10975Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base T (13/15);  minor base A (1/2);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGAATGGATTGATTGCGATCCATCTCTTTGGCGAGTTTGAATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATA  >  CP006881/2190247‑2190510
                                                                                                                                       |                                                                                                                                
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATTCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  3:436588/139‑1 (MQ=255)
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  5:358636/139‑1 (MQ=255)
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  4:439402/139‑1 (MQ=255)
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  3:847/139‑1 (MQ=255)
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  3:434367/139‑1 (MQ=255)
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  3:11168/139‑1 (MQ=255)
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  2:430335/139‑1 (MQ=255)
gagaATGAGTTGACCGCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  2:256668/139‑1 (MQ=255)
            accgCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATaa                                                                                                                      >  1:12722/4‑136 (MQ=255)
            accgCGATCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGAAACCACCGCTTCTTCATCGGGAATACTGTCATAAGt                                                                                                                    >  3:49478/4‑138 (MQ=255)
                  aTCCATTCCTTTGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACgg                                                                                                             >  5:269017/1‑139 (MQ=255)
                   tCCATTCCTTTGGCGAGTTTTAATGCTTTCGATAACGAATGGGCAGATTCGATTGCCGTCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCAACCACCGCTTCTTCATCGGTTATACTGTAATAAGTGACACGGc                                                                                                            <  6:269017/139‑1 (MQ=255)
                             tGGCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATg                                                                                                  >  5:305967/1‑139 (MQ=255)
                              ggCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGAAAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATg                                                                                                  <  2:40423/138‑1 (MQ=255)
                               gCGAGTTTGAATGCTTTCGCTACCGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGCCAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCa                                                                                                <  4:132443/139‑1 (MQ=255)
                               gCGAGTTTGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGc                                                                                                 >  1:306405/1‑138 (MQ=255)
                               gCGAGGTTGAATGCGTTCGCTAACGCTTGGGCAGATGCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATCCATCCACCGCAGCTTCATCGGTTCTACTGTCATAAGTGACACGGCCGCTTTTATGCa                                                                                                <  3:226745/139‑1 (MQ=255)
                                   gTTTGAATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAata                                                                                            >  8:122311/1‑139 (MQ=255)
                                    tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               <  5:29430/103‑1 (MQ=255)
                                    tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTtata                                                                                                                               >  6:29430/1‑103 (MQ=255)
                                    tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTTTGCAAaaat                                                                                           >  1:140144/1‑139 (MQ=255)
                                    tttGAATGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATTGAAataa                                                                                           >  6:269143/1‑138 (MQ=255)
                                         aTGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATTCAAATATGCATg                                                                                      >  7:274911/1‑139 (MQ=255)
                                                       gCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTc                                                                        <  7:122311/139‑1 (MQ=255)
                                                           gggCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCTGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATTCATTGTCCGGACCGATTCGAgg                                                                    >  3:280094/1‑139 (MQ=255)
                                                           gggCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATAATGAAGCGCCGGACCGATTCCAg                                                                     >  5:146348/1‑138 (MQ=255)
                                                                     gATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga                                                          <  5:167694/139‑2 (MQ=255)
                                                                         ggcGGCAAAATCGCGTCTTTTTCTGACATAAGCTTTAATGAATCAACCGCTTCTTCATCGGTTATAGTGTAATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAcc                                                        <  1:205436/135‑1 (MQ=255)
                                                                          ccgcaaaaaGCCCCTCTTTTTCTGAAAAATGTTTTATAGCATCCCCCGGTTCTTCATCGGTTTTACTGTCATAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggc                                                     <  5:52761/134‑1 (MQ=255)
                                                                          ccGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg                                                      <  3:169507/138‑3 (MQ=255)
                                                                            ggCAAAATCCCCTCTTTTTCTGACAACAGCTTTAATCCATCCACCGCTTCTTCATCGGTAATACTGTCATCAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg                                                   <  8:57178/139‑1 (MQ=255)
                                                                            ggCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg                                                   <  2:306405/139‑1 (MQ=255)
                                                                               aaaaTCCCCTCTTTTTCTTACAAATCCTTTAATGCAACCCCCGCTTCTTCATGGTTTATACTGTCATACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa                                                <  5:269143/139‑1 (MQ=255)
                                                                               aaaaTCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa                                                <  2:12722/139‑1 (MQ=255)
                                                                                      ccTCTTTTTCTGACAAAACCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa                                         <  3:210219/139‑1 (MQ=255)
                                                                                      ccTCTTTATCTCACAAAAGCTTTATTGCCCCCACAGCTTATCCTTCGGTTAACCGGCCAAAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa                                         <  4:99989/139‑1 (MQ=255)
                                                                                                  aCAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGCCGAGACCGGCTGAAATAGAGTAGGGCTCaataat                             >  3:218900/1‑139 (MQ=255)
                                                                                                                   tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGGATGCTGCGGACCGATTGCAGGATTGTCGAGACCGGCTGAAATAGAGTAGGGCGCAATAATTTGCCCGAACTcctgcg            >  4:412151/1‑134 (MQ=255)
                                                                                                                   tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGa                                                                   >  1:100868/1‑84 (MQ=255)
                                                                                                                   tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGa                                                                   <  2:100868/84‑1 (MQ=255)
                                                                                                                   tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATcct            >  7:10417/1‑137 (MQ=255)
                                                                                                                      aCCGCTTCTTCTTCGGTTAAACTGTATTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGaa         <  4:199815/139‑1 (MQ=255)
                                                                                                                             cttcATCGGGAATACAGGCATAAGTGCCACGGCCGCTTTAATGCAAATATGCATGCTCCGGACCGATTCCAGCATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATa  <  2:233334/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                
GAGAATGGATTGATTGCGATCCATCTCTTTGGCGAGTTTGAATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATA  >  CP006881/2190247‑2190510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑