| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,190,424 | G→A | H315H (CAC→CAT) | U712_10975 ← | Tryptophan synthase beta chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,190,424 | 0 | G | A | 97.4% | 107.3 / NA | 40 | H315H (CAC→CAT) | U712_10975 | Tryptophan synthase beta chain |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (17/21); minor base C (1/0); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTA > CP006881/2190288‑2190556 | aTGCTTTCGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATTCAAATATGCATg > 7:274911/1‑139 (MQ=255) gCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTc < 7:122311/139‑1 (MQ=255) gggCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCTGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATTCATTGTCCGGACCGATTCGAgg > 3:280094/1‑139 (MQ=255) gggCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATAATGAAGCGCCGGACCGATTCCAg > 5:146348/1‑138 (MQ=255) gATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga < 5:167694/139‑2 (MQ=255) ggcGGCAAAATCGCGTCTTTTTCTGACATAAGCTTTAATGAATCAACCGCTTCTTCATCGGTTATAGTGTAATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAcc < 1:205436/135‑1 (MQ=255) ccgcaaaaaGCCCCTCTTTTTCTGAAAAATGTTTTATAGCATCCCCCGGTTCTTCATCGGTTTTACTGTCATAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggc < 5:52761/134‑1 (MQ=255) ccGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg < 3:169507/138‑3 (MQ=255) ggCAAAATCCCCTCTTTTTCTGACAACAGCTTTAATCCATCCACCGCTTCTTCATCGGTAATACTGTCATCAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg < 8:57178/139‑1 (MQ=255) ggCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg < 2:306405/139‑1 (MQ=255) aaaaTCCCCTCTTTTTCTTACAAATCCTTTAATGCAACCCCCGCTTCTTCATGGTTTATACTGTCATACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa < 5:269143/139‑1 (MQ=255) aaaaTCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa < 2:12722/139‑1 (MQ=255) ccTCTTTTTCTGACAAAACCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa < 3:210219/139‑1 (MQ=255) ccTCTTTATCTCACAAAAGCTTTATTGCCCCCACAGCTTATCCTTCGGTTAACCGGCCAAAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa < 4:99989/139‑1 (MQ=255) aCAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGCCGAGACCGGCTGAAATAGAGTAGGGCTCaataat > 3:218900/1‑139 (MQ=255) tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGGATGCTGCGGACCGATTGCAGGATTGTCGAGACCGGCTGAAATAGAGTAGGGCGCAATAATTTGCCCGAACTcctgcg > 4:412151/1‑134 (MQ=255) tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGa > 1:100868/1‑84 (MQ=255) tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGa < 2:100868/84‑1 (MQ=255) tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATcct > 7:10417/1‑137 (MQ=255) aCCGCTTCTTCTTCGGTTAAACTGTATTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGaa < 4:199815/139‑1 (MQ=255) cttcATCGGGAATACAGGCATAAGTGCCACGGCCGCTTTAATGCAAATATGCATGCTCCGGACCGATTCCAGCATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATa < 2:233334/139‑1 (MQ=255) tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc < 8:10417/138‑1 (MQ=255) tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCGCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc < 6:305967/138‑1 (MQ=255) cATAAGTGACACGGCCGCTTTTATGCAAATATGCCTGCTCCGGCCCCAATCCAGGATAGTAGAGCCCGGCGGAAATAGAGTAGTGCGCAAAAATTTGCCCGGACGCATCCGGCATGAGAGAAGTCAAAGAACCggtaa > 6:206862/1‑134 (MQ=255) aTACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGCCCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt < 5:206862/138‑1 (MQ=255) aTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt > 8:82931/1‑138 (MQ=255) aTAAGTGACACGGCCGCTTTTAATCAAAAATTGATGCTCCGGACCGATACCACGCTAGTACCGACCGGCTGTAATATACTAGGGGTCAATAATATCAGCGCAAGCAACCAGAATGAGATAAGTCAATTAACCGAGaaa > 4:437794/1‑137 (MQ=255) aTAAGTGACACGGCCGCGTTTATGCAAATATGCATGCGACGGCCCGATTCCCGGATAGTCGAGACCGGCAGAAATAGAGTAGGGCGCTATATTTTGACCGTACTAAGCCTGCTTGAGAATAGTCAATGAACCGTGaca > 6:104618/1‑136 (MQ=255) aGTGACACGGCCGCTTTTATGCAAATATTCATGCTCCGGACCGATTCCAGGATATTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTTCCCGAACACATCCTGAATTAGATAAGTAAATTAAACCGGAATAAcc > 8:329988/1‑138 (MQ=255) aGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAAc < 7:165399/138‑1 (MQ=255) cacGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga < 1:149878/56‑2 (MQ=255) cacGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga > 2:149878/1‑55 (MQ=255) cacGGCCGCTTTTATGCAAATATGCATGCTCAGGTCCGATTGCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACACATCCTGAATGACATAAGTAAATGAAACGTGAATAACCCCTAc > 8:152479/1‑139 (MQ=255) tATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCg < 2:140144/138‑1 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg > 8:87431/1‑138 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg > 6:413888/1‑39 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg < 5:413888/41‑3 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATGGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg > 3:281060/1‑138 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg < 2:127228/106‑1 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg > 1:127228/1‑106 (MQ=255) | ATGCTTTTGCTAACGCATGGGCAGATTCGATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTA > CP006881/2190288‑2190556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |