| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,190,451 | A→G | L306L (CTT→CTC) | U712_10975 ← | Tryptophan synthase beta chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,190,451 | 0 | A | G | 98.2% | 175.4 / NA | 55 | L306L (CTT→CTC) | U712_10975 | Tryptophan synthase beta chain |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (27/27); minor base C (1/0); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTT > CP006881/2190316‑2190586 | gATTGCCGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga < 5:167694/139‑2 (MQ=255) ggcGGCAAAATCGCGTCTTTTTCTGACATAAGCTTTAATGAATCAACCGCTTCTTCATCGGTTATAGTGTAATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAcc < 1:205436/135‑1 (MQ=255) ccgcaaaaaGCCCCTCTTTTTCTGAAAAATGTTTTATAGCATCCCCCGGTTCTTCATCGGTTTTACTGTCATAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggc < 5:52761/134‑1 (MQ=255) ccGGCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg < 3:169507/138‑3 (MQ=255) ggCAAAATCCCCTCTTTTTCTGACAACAGCTTTAATCCATCCACCGCTTCTTCATCGGTAATACTGTCATCAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg < 8:57178/139‑1 (MQ=255) ggCAAAATCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACcggctg < 2:306405/139‑1 (MQ=255) aaaaTCCCCTCTTTTTCTTACAAATCCTTTAATGCAACCCCCGCTTCTTCATGGTTTATACTGTCATACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa < 5:269143/139‑1 (MQ=255) aaaaTCCCCTCTTTTTCTGACAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGaaa < 2:12722/139‑1 (MQ=255) ccTCTTTTTCTGACAAAACCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa < 3:210219/139‑1 (MQ=255) ccTCTTTATCTCACAAAAGCTTTATTGCCCCCACAGCTTATCCTTCGGTTAACCGGCCAAAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa < 4:99989/139‑1 (MQ=255) aCAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGCCGAGACCGGCTGAAATAGAGTAGGGCTCaataat > 3:218900/1‑139 (MQ=255) tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGGATGCTGCGGACCGATTGCAGGATTGTCGAGACCGGCTGAAATAGAGTAGGGCGCAATAATTTGCCCGAACTcctgcg > 4:412151/1‑134 (MQ=255) tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATcct > 7:10417/1‑137 (MQ=255) aCCGCTTCTTCTTCGGTTAAACTGTATTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGaa < 4:199815/139‑1 (MQ=255) cttcATCGGGAATACAGGCATAAGTGCCACGGCCGCTTTAATGCAAATATGCATGCTCCGGACCGATTCCAGCATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATa < 2:233334/139‑1 (MQ=255) tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc < 8:10417/138‑1 (MQ=255) tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCGCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc < 6:305967/138‑1 (MQ=255) cATAAGTGACACGGCCGCTTTTATGCAAATATGCCTGCTCCGGCCCCAATCCAGGATAGTAGAGCCCGGCGGAAATAGAGTAGTGCGCAAAAATTTGCCCGGACGCATCCGGCATGAGAGAAGTCAAAGAACCggtaa > 6:206862/1‑134 (MQ=255) aTACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGCCCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt < 5:206862/138‑1 (MQ=255) aTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt > 8:82931/1‑138 (MQ=255) aTAAGTGACACGGCCGCTTTTAATCAAAAATTGATGCTCCGGACCGATACCACGCTAGTACCGACCGGCTGTAATATACTAGGGGTCAATAATATCAGCGCAAGCAACCAGAATGAGATAAGTCAATTAACCGAGaaa > 4:437794/1‑137 (MQ=255) aTAAGTGACACGGCCGCGTTTATGCAAATATGCATGCGACGGCCCGATTCCCGGATAGTCGAGACCGGCAGAAATAGAGTAGGGCGCTATATTTTGACCGTACTAAGCCTGCTTGAGAATAGTCAATGAACCGTGaca > 6:104618/1‑136 (MQ=255) aGTGACACGGCCGCTTTTATGCAAATATTCATGCTCCGGACCGATTCCAGGATATTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTTCCCGAACACATCCTGAATTAGATAAGTAAATTAAACCGGAATAAcc > 8:329988/1‑138 (MQ=255) aGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAAc < 7:165399/138‑1 (MQ=255) cacGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga > 2:149878/1‑55 (MQ=255) cacGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAga < 1:149878/56‑2 (MQ=255) cacGGCCGCTTTTATGCAAATATGCATGCTCAGGTCCGATTGCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACACATCCTGAATGACATAAGTAAATGAAACGTGAATAACCCCTAc > 8:152479/1‑139 (MQ=255) tATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCg < 2:140144/138‑1 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg > 8:87431/1‑138 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg < 5:413888/41‑3 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGAccgg > 6:413888/1‑39 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATGGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg > 3:281060/1‑138 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg > 1:127228/1‑106 (MQ=255) atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg < 2:127228/106‑1 (MQ=255) catGCTCCGGACCGATTCCAGGCTAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTggcggc < 8:274911/137‑1 (MQ=255) catGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAAAGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCgg > 2:317064/3‑138 (MQ=255) gCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCa < 1:227698/90‑1 (MQ=255) gCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCa > 2:227698/1‑90 (MQ=255) cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg > 8:157328/1‑138 (MQ=255) cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg < 7:329988/138‑1 (MQ=255) cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGa < 2:261321/139‑1 (MQ=255) tCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg > 8:47687/1‑137 (MQ=255) tCCGGACCGATTCCAGGAAAGTCGAGACCGGCTGAAATAGAGTAGGGCTAAATAATATGCCCGAACTCATCCTGAATGAGATAAGTCAATGCACCGTGAATACCCCCTAAGGTTCCTTTCGAAATAGTGGCGGCTTGaa > 4:370891/1‑139 (MQ=255) ccGGACCTATTGCTGGTTACTCGAGCCCGGCTGAAATAGAGGAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGTGTAAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAATGTGGCGGCATGAag < 6:152965/139‑1 (MQ=255) ccGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAag < 7:157328/139‑1 (MQ=255) aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt > 1:37037/1‑92 (MQ=255) aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt < 2:37037/92‑1 (MQ=255) aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTa > 4:45283/1‑138 (MQ=255) aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt > 2:242685/1‑139 (MQ=255) aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCACTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt > 7:84913/1‑139 (MQ=255) ttCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt > 1:74280/1‑138 (MQ=255) ttCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt > 1:139568/1‑138 (MQ=255) aGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt > 5:74120/1‑139 (MQ=255) gTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCtttt > 6:396254/1‑139 (MQ=255) gTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt > 4:169748/1‑138 (MQ=255) | GATTGCCGGCAAAATCCCCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTT > CP006881/2190316‑2190586 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |