Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,190,466 G→A S301S (TCC→TCT)  U712_10975 ← Tryptophan synthase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,190,4660GA98.2% 158.6 / ‑4.5 54S301S (TCC→TCT) U712_10975Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (33/20);  total (34/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

CCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTTCCGGCTGCTTCAGCG  >  CP006881/2190333‑2190601
                                                                                                                                     |                                                                                                                                       
ccTCTTTTTCTGACAAAACCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa                                                                                                                                    <  3:210219/139‑1 (MQ=255)
ccTCTTTATCTCACAAAAGCTTTATTGCCCCCACAGCTTATCCTTCGGTTAACCGGCCAAAAGTCACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTa                                                                                                                                    <  4:99989/139‑1 (MQ=255)
            aCAAAAGCTTTAATGCATCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGCCGAGACCGGCTGAAATAGAGTAGGGCTCaataat                                                                                                                        >  3:218900/1‑139 (MQ=255)
                             tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGGATGCTGCGGACCGATTGCAGGATTGTCGAGACCGGCTGAAATAGAGTAGGGCGCAATAATTTGCCCGAACTcctgcg                                                                                                       >  4:412151/1‑134 (MQ=255)
                             tCCACCGCTTCTTCATCGGTTATACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATcct                                                                                                       >  7:10417/1‑137 (MQ=255)
                                aCCGCTTCTTCTTCGGTTAAACTGTATTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGaa                                                                                                    <  4:199815/139‑1 (MQ=255)
                                       cttcATCGGGAATACAGGCATAAGTGCCACGGCCGCTTTAATGCAAATATGCATGCTCCGGACCGATTCCAGCATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATa                                                                                             <  2:233334/139‑1 (MQ=255)
                                                   tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc                                                                                  <  8:10417/138‑1 (MQ=255)
                                                   tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCGCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc                                                                                  <  6:305967/138‑1 (MQ=255)
                                                         cATAAGTGACACGGCCGCTTTTATGCAAATATGCCTGCTCCGGCCCCAATCCAGGATAGTAGAGCCCGGCGGAAATAGAGTAGTGCGCAAAAATTTGCCCGGACGCATCCGGCATGAGAGAAGTCAAAGAACCggtaa                                                                            >  6:206862/1‑134 (MQ=255)
                                                          aTACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGCCCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                           <  5:206862/138‑1 (MQ=255)
                                                          aTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                           >  8:82931/1‑138 (MQ=255)
                                                          aTAAGTGACACGGCCGCTTTTAATCAAAAATTGATGCTCCGGACCGATACCACGCTAGTACCGACCGGCTGTAATATACTAGGGGTCAATAATATCAGCGCAAGCAACCAGAATGAGATAAGTCAATTAACCGAGaaa                                                                           >  4:437794/1‑137 (MQ=255)
                                                          aTAAGTGACACGGCCGCGTTTATGCAAATATGCATGCGACGGCCCGATTCCCGGATAGTCGAGACCGGCAGAAATAGAGTAGGGCGCTATATTTTGACCGTACTAAGCCTGCTTGAGAATAGTCAATGAACCGTGaca                                                                           >  6:104618/1‑136 (MQ=255)
                                                             aGTGACACGGCCGCTTTTATGCAAATATTCATGCTCCGGACCGATTCCAGGATATTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTTCCCGAACACATCCTGAATTAGATAAGTAAATTAAACCGGAATAAcc                                                                       >  8:329988/1‑138 (MQ=255)
                                                             aGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAAc                                                                        <  7:165399/138‑1 (MQ=255)
                                                                  cacGGCCGCTTTTATGCAAATATGCATGCTCAGGTCCGATTGCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACACATCCTGAATGACATAAGTAAATGAAACGTGAATAACCCCTAc                                                                  >  8:152479/1‑139 (MQ=255)
                                                                              tATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCg                                                       <  2:140144/138‑1 (MQ=255)
                                                                                     atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg                                               >  8:87431/1‑138 (MQ=255)
                                                                                     atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATGGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg                                               >  3:281060/1‑138 (MQ=255)
                                                                                     atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg                                                                                >  1:127228/1‑106 (MQ=255)
                                                                                     atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg                                                                                <  2:127228/106‑1 (MQ=255)
                                                                                          catGCTCCGGACCGATTCCAGGCTAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTggcggc                                          <  8:274911/137‑1 (MQ=255)
                                                                                          catGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAAAGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCgg                                           >  2:317064/3‑138 (MQ=255)
                                                                                             gCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCa                                                                                        <  1:227698/90‑1 (MQ=255)
                                                                                             gCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCa                                                                                        >  2:227698/1‑90 (MQ=255)
                                                                                              cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg                                       >  8:157328/1‑138 (MQ=255)
                                                                                              cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg                                       <  7:329988/138‑1 (MQ=255)
                                                                                              cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGa                                      <  2:261321/139‑1 (MQ=255)
                                                                                               tCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg                                       >  8:47687/1‑137 (MQ=255)
                                                                                               tCCGGACCGATTCCAGGAAAGTCGAGACCGGCTGAAATAGAGTAGGGCTAAATAATATGCCCGAACTCATCCTGAATGAGATAAGTCAATGCACCGTGAATACCCCCTAAGGTTCCTTTCGAAATAGTGGCGGCTTGaa                                     >  4:370891/1‑139 (MQ=255)
                                                                                                ccGGACCTATTGCTGGTTACTCGAGCCCGGCTGAAATAGAGGAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGTGTAAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAATGTGGCGGCATGAag                                    <  6:152965/139‑1 (MQ=255)
                                                                                                ccGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAag                                    <  7:157328/139‑1 (MQ=255)
                                                                                                        aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                           <  2:37037/92‑1 (MQ=255)
                                                                                                        aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                           >  1:37037/1‑92 (MQ=255)
                                                                                                        aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTa                             >  4:45283/1‑138 (MQ=255)
                                                                                                        aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                            >  2:242685/1‑139 (MQ=255)
                                                                                                        aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCACTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                            >  7:84913/1‑139 (MQ=255)
                                                                                                         ttCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                            >  1:139568/1‑138 (MQ=255)
                                                                                                         ttCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                            >  1:74280/1‑138 (MQ=255)
                                                                                                                  aGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt                  >  5:74120/1‑139 (MQ=255)
                                                                                                                   gTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCtttt                 >  6:396254/1‑139 (MQ=255)
                                                                                                                   gTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt                  >  4:169748/1‑138 (MQ=255)
                                                                                                                        gaccggctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTgc            <  4:218900/134‑1 (MQ=255)
                                                                                                                        gaccggctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTgc            <  3:412151/134‑1 (MQ=255)
                                                                                                                         accggctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTgct           >  2:115127/5‑139 (MQ=255)
                                                                                                                             gctgAAATATAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCtt       >  1:29384/1‑139 (MQ=255)
                                                                                                                             gctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCg                                            >  2:100598/1‑102 (MQ=255)
                                                                                                                             gctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCg                                            <  1:100598/102‑1 (MQ=255)
                                                                                                                              ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCtt       >  2:43208/1‑138 (MQ=255)
                                                                                                                              ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTc      >  3:171551/1‑139 (MQ=255)
                                                                                                                              ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAAAACCCCTACCGTTCCTTTCGAAATAGTGGAGGCATGAAGAGGTGTATCAATACCTTTTCCTGCTGCtt       >  8:224153/1‑138 (MQ=255)
                                                                                                                              ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGACAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGCTGTATCAATTCC‑TTTCCTGCTGCtt       >  4:255057/1‑137 (MQ=255)
                                                                                                                                  aaTAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAgcg  >  8:359627/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                       
CCTCTTTTTCTGACAAGAGTTTTAATGCATCCACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTTCCGGCTGCTTCAGCG  >  CP006881/2190333‑2190601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑