Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,190,499	T→C	Q290Q (CAA→CAG)	U712_10975 ←	Tryptophan synthase beta chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,190,499	0	T	C	100.0%	256.4 / NA	78	Q290Q (CAA→CAG)	U712_10975	Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/31);  total (47/31)

ACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTTCCGGCTGCTTCAGCGCCGATCAGTTCAACATCTTCGTCTAAAAACGCC  >  CP006881/2190365‑2190634                                                                                                                                       |                                                                                                                                        aCCGCTTCTTCTTCGGTTAAACTGTATTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGaa                                                                                                                                     <  4:199815/139‑1 (MQ=255)        cttcATCGGGAATACAGGCATAAGTGCCACGGCCGCTTTAATGCAAATATGCATGCTCCGGACCGATTCCAGCATAGGCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATa                                                                                                                              <  2:233334/139‑1 (MQ=255)                    tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc                                                                                                                   <  8:10417/138‑1 (MQ=255)                    tACTGTCATAAGTGACACGGCCGCTTTTATGCAAATATGCATGCGCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAAc                                                                                                                   <  6:305967/138‑1 (MQ=255)                          cATAAGTGACACGGCCGCTTTTATGCAAATATGCCTGCTCCGGCCCCAATCCAGGATAGTAGAGCCCGGCGGAAATAGAGTAGTGCGCAAAAATTTGCCCGGACGCATCCGGCATGAGAGAAGTCAAAGAACCggtaa                                                                                                             >  6:206862/1‑134 (MQ=255)                           aTACGTGCCACGGCCGCTTTTATGCAAATATGCATGCTCCGGCCCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                                                            <  5:206862/138‑1 (MQ=255)                           aTAAGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                                                            >  8:82931/1‑138 (MQ=255)                           aTAAGTGACACGGCCGCTTTTAATCAAAAATTGATGCTCCGGACCGATACCACGCTAGTACCGACCGGCTGTAATATACTAGGGGTCAATAATATCAGCGCAAGCAACCAGAATGAGATAAGTCAATTAACCGAGaaa                                                                                                            >  4:437794/1‑137 (MQ=255)                           aTAAGTGACACGGCCGCGTTTATGCAAATATGCATGCGACGGCCCGATTCCCGGATAGTCGAGACCGGCAGAAATAGAGTAGGGCGCTATATTTTGACCGTACTAAGCCTGCTTGAGAATAGTCAATGAACCGTGaca                                                                                                            >  6:104618/1‑136 (MQ=255)                              aGTGACACGGCCGCTTTTATGCAAATATTCATGCTCCGGACCGATTCCAGGATATTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTTCCCGAACACATCCTGAATTAGATAAGTAAATTAAACCGGAATAAcc                                                                                                        >  8:329988/1‑138 (MQ=255)                              aGTGACACGGCCGCTTTTATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAAc                                                                                                         <  7:165399/138‑1 (MQ=255)                                   cacGGCCGCTTTTATGCAAATATGCATGCTCAGGTCCGATTGCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACACATCCTGAATGACATAAGTAAATGAAACGTGAATAACCCCTAc                                                                                                   >  8:152479/1‑139 (MQ=255)                                               tATGCAAATATGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCg                                                                                        <  2:140144/138‑1 (MQ=255)                                                      atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg                                                                                >  8:87431/1‑138 (MQ=255)                                                      atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATGGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAtagtg                                                                                >  3:281060/1‑138 (MQ=255)                                                      atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg                                                                                                                 >  1:127228/1‑106 (MQ=255)                                                      atatGCATGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCg                                                                                                                 <  2:127228/106‑1 (MQ=255)                                                           catGCTCCGGACCGATTCCAGGCTAGTCGAGCCCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTggcggc                                                                           <  8:274911/137‑1 (MQ=255)                                                           catGCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAAAGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCgg                                                                            >  2:317064/3‑138 (MQ=255)                                                              gCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCa                                                                                                                         <  1:227698/90‑1 (MQ=255)                                                              gCTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCa                                                                                                                         >  2:227698/1‑90 (MQ=255)                                                               cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg                                                                        >  8:157328/1‑138 (MQ=255)                                                               cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg                                                                        <  7:329988/138‑1 (MQ=255)                                                               cTCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGa                                                                       <  2:261321/139‑1 (MQ=255)                                                                tCCGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATg                                                                        >  8:47687/1‑137 (MQ=255)                                                                tCCGGACCGATTCCAGGAAAGTCGAGACCGGCTGAAATAGAGTAGGGCTAAATAATATGCCCGAACTCATCCTGAATGAGATAAGTCAATGCACCGTGAATACCCCCTAAGGTTCCTTTCGAAATAGTGGCGGCTTGaa                                                                      >  4:370891/1‑139 (MQ=255)                                                                 ccGGACCTATTGCTGGTTACTCGAGCCCGGCTGAAATAGAGGAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGTGTAAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAAATGTGGCGGCATGAag                                                                     <  6:152965/139‑1 (MQ=255)                                                                 ccGGACCGATTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAag                                                                     <  7:157328/139‑1 (MQ=255)                                                                         aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                                                            <  2:37037/92‑1 (MQ=255)                                                                         aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                                                            >  1:37037/1‑92 (MQ=255)                                                                         aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTa                                                              >  4:45283/1‑138 (MQ=255)                                                                         aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                                                             >  2:242685/1‑139 (MQ=255)                                                                         aTTCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCACTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                                                             >  7:84913/1‑139 (MQ=255)                                                                          ttCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                                                             >  1:74280/1‑138 (MQ=255)                                                                          ttCCAGGATAGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTAt                                                             >  1:139568/1‑138 (MQ=255)                                                                                   aGTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt                                                   >  5:74120/1‑139 (MQ=255)                                                                                    gTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCtttt                                                  >  6:396254/1‑139 (MQ=255)                                                                                    gTCGAGACCGGCTGAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt                                                   >  4:169748/1‑138 (MQ=255)                                                                                         gaccggctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTgc                                             <  4:218900/134‑1 (MQ=255)                                                                                         gaccggctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTgc                                             <  3:412151/134‑1 (MQ=255)                                                                                          accggctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTgct                                            >  2:115127/5‑139 (MQ=255)                                                                                              gctgAAATATAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCtt                                        >  1:29384/1‑139 (MQ=255)                                                                                              gctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCg                                                                             <  1:100598/102‑1 (MQ=255)                                                                                              gctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCg                                                                             >  2:100598/1‑102 (MQ=255)                                                                                               ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCtt                                        >  2:43208/1‑138 (MQ=255)                                                                                               ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTc                                       >  3:171551/1‑139 (MQ=255)                                                                                               ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAAAACCCCTACCGTTCCTTTCGAAATAGTGGAGGCATGAAGAGGTGTATCAATACCTTTTCCTGCTGCtt                                        >  8:224153/1‑138 (MQ=255)                                                                                               ctgAAATAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGACAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGCTGTATCAATTCC‑TTTCCTGCTGCtt                                        >  4:255057/1‑137 (MQ=255)                                                                                                   aaTAGAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAgcg                                   >  8:359627/1‑139 (MQ=255)                                                                                                       gAGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCg                                <  7:82931/138‑1 (MQ=255)                                                                                                        tgTAGGGCTCATTAATTAGACAGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTAAGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGAGTATCAATTCCTTTTCCTTCTGCTTCAGCGCCGAt                              <  5:104618/138‑1 (MQ=255)                                                                                                        aGTAGGGCTCAATAATTTGCCCGACCTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACTGTTCCTTTCGAATTAGTGGCGGCATGATGAGGTGTATCAAGTCCTTTTCCTGCTGCTTCAGCGCCGAt                              >  7:275247/1‑139 (MQ=255)                                                                                                        aGTAGGGCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGAt                              <  7:47687/139‑1 (MQ=255)                                                                                                             ggCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                                                            >  1:272077/1‑56 (MQ=255)                                                                                                             ggCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAAt                                                                                                            <  2:272077/56‑1 (MQ=255)                                                                                                             ggCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGAt                              >  7:289789/1‑134 (MQ=255)                                                                                                             ggCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGAt                              <  8:289789/134‑1 (MQ=255)                                                                                                             ggCTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGtt                         >  4:52142/1‑139 (MQ=255)                                                                                                               cTCAATAATTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTc                        >  5:68323/1‑138 (MQ=255)                                                                                                                  aataatTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGtt                                                                                               <  8:97961/64‑1 (MQ=255)                                                                                                                  aataatTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGtt                                                                                               >  7:97961/1‑64 (MQ=255)                                                                                                                  aataatTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACa                    >  4:8008/1‑139 (MQ=255)                                                                                                                  aataatTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACa                    >  1:163763/1‑139 (MQ=255)                                                                                                                  aataatTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACa                    >  5:129984/1‑139 (MQ=255)                                                                                                                      atTTGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCt                 <  1:242685/138‑1 (MQ=255)                                                                                                                       tttGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt                                                   <  2:297568/103‑1 (MQ=255)                                                                                                                       tttGCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCttt                                                   >  1:297568/1‑103 (MQ=255)                                                                                                                          gCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTcatt            >  6:272245/1‑136 (MQ=255)                                                                                                                          gCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTcatt            >  8:76933/1‑136 (MQ=255)                                                                                                                          gCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAACAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAag                                                                     >  4:181757/1‑82 (MQ=255)                                                                                                                          gCCCGAACTCATCCTGAATGAGATAAGTCAATGAACCGTGAACAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAag                                                                     <  3:181757/82‑1 (MQ=255)                                                                                                                               aaCTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGAt                              <  8:372443/116‑1 (MQ=255)                                                                                                                               aaCTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGAt                              >  7:372443/1‑116 (MQ=255)                                                                                                                               aaCTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTaaa        >  7:52458/1‑138 (MQ=255)                                                                                                                                aCTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGATCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATATaaaaa      >  5:383798/1‑139 (MQ=255)                                                                                                                                    aTCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGcc  <  7:152479/139‑1 (MQ=255)                                                                                                                                    aTCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGc   <  6:129984/138‑1 (MQ=255)                                                                                                                                    aTCCTGAAAGAGATAAGGCAATGAACCTTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGc   <  7:269073/138‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ACCGCTTCTTCATCTGTGATACTGTCATAAGTGACACGGCCGCTCTTATGCAAATATGCGTGCTCCGGACCGATTCCAGGATAGTCAAGTCCTGCTGAAATGGAGTAAGGCTCAATAATTTGCCCGAACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTTCCGGCTGCTTCAGCGCCGATCAGTTCAACATCTTCGTCTAAAAACGCC  >  CP006881/2190365‑2190634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑