Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,190,622	G→A	D249N (GAC→AAT)	U712_10975 ←	Tryptophan synthase beta chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,190,622	0	G	A	100.0%	295.6 / NA	93	D249N (GAC→AAT)	U712_10975	Tryptophan synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/49);  total (44/49)

AACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTTCCGGCTGCTTCAGCGCCGATCAGTTCAACATCTTCGTCTAAAAACGCCTGAAACATGCCTATCGCATTGCTTCCTCCACCTACACACGCCACTACTTTATCAGGCAGAGTGCCTTCAATGTGTTTCAGCTGATCCTTCGCTTCCTCTCCAATCATTTTCTGAAATTCT  >  CP006881/2190491‑2190754                                                                                                                                    |                                                                                                                                     aaCTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTaaa                                                                                                                                >  7:52458/1‑138 (MQ=255)  aCTCATCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGATCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATATaaaaa                                                                                                                              >  5:383798/1‑139 (MQ=255)      aTCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGcc                                                                                                                          <  7:152479/139‑1 (MQ=255)      aTCCTGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGc                                                                                                                           <  6:129984/138‑1 (MQ=255)      aTCCTGAAAGAGATAAGGCAATGAACCTTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGc                                                                                                                           <  7:269073/138‑1 (MQ=255)       tcctGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         <  6:74120/136‑1 (MQ=255)       tcctGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         <  2:29384/136‑1 (MQ=255)       tcctGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         <  4:281060/136‑1 (MQ=255)       tcctGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         <  7:76933/136‑1 (MQ=255)        cctGAATGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         >  5:130295/3‑138 (MQ=255)            aaTGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGaaa                                                                                                                     >  2:109555/1‑138 (MQ=255)            aaTGAGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGaaa                                                                                                                     >  3:442039/1‑138 (MQ=255)             atgaGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACa                                                                                                                   <  3:8008/136‑1 (MQ=255)                aGATAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTAGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGAGCAGTTCAACATCTTCATTTAAAAAAGCCTGAAACAtac                                                                                                                >  8:423624/1‑137 (MQ=255)                  aTAAGTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCATTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATAcc                                                                                                               >  8:193238/1‑138 (MQ=255)                      gTCAATGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATCCCCATCg                                                                                                          >  7:381978/1‑139 (MQ=255)                         aaTGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCTGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCa                                                                                                        <  8:374104/138‑1 (MQ=255)                          aTGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCAtt                                                                                                      >  1:155552/1‑139 (MQ=255)                           tGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTg                                                                                                     >  4:177137/1‑139 (MQ=255)                           tGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTg                                                                                                     >  5:276246/1‑139 (MQ=255)                           tGAACCGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTg                                                                                                     >  5:405140/1‑139 (MQ=255)                                cGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTtcc                                                                                                <  7:359627/139‑1 (MQ=255)                                cGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTtcc                                                                                                <  6:146348/139‑1 (MQ=255)                                cGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTtcc                                                                                                <  1:317064/139‑1 (MQ=255)                                cGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTtcc                                                                                                >  1:310526/1‑139 (MQ=255)                                cGTGAATAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTtcc                                                                                                <  8:84913/139‑1 (MQ=255)                                    aaTAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACa                                                                                                                   >  1:221757/1‑116 (MQ=255)                                    aaTAACCCCTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACa                                                                                                                   <  2:221757/116‑1 (MQ=255)                                          cccTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTaca                                                                                      >  7:23643/2‑139 (MQ=255)                                          cccTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTaca                                                                                      <  6:68323/138‑1 (MQ=255)                                          cccTACGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTaca                                                                                      <  2:163763/138‑1 (MQ=255)                                              aCGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         >  7:223017/1‑100 (MQ=255)                                              aCGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         <  8:223017/100‑1 (MQ=255)                                              aCGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCGTCCGCCTacacatg                                                                                  >  1:227254/1‑137 (MQ=255)                                              aCGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTacacatg                                                                                  >  2:276254/1‑137 (MQ=255)                                              aCGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTaca                                                                                      >  1:292006/1‑135 (MQ=255)                                              aCGGTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTaca                                                                                      <  2:292006/135‑1 (MQ=255)                                               cGGTTCCTTTCGTAATAGTGGCGGGATGAAGACGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGc                                                                                 <  4:49478/139‑1 (MQ=255)                                                 gTTCCTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCa                                                                               <  1:115127/139‑1 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTaaaa                                                                                                                               <  7:11875/88‑1 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTaaaa                                                                                                                               >  8:11875/1‑88 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTtcctcc                                                                                             <  2:397821/122‑1 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTtcctcc                                                                                             >  1:397821/1‑122 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCacta                                                                            >  6:423788/1‑139 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCacta                                                                            >  6:126017/1‑139 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCacta                                                                            >  4:18022/1‑139 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCacta                                                                            >  3:259614/1‑139 (MQ=255)                                                    ccTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCact                                                                             >  1:85962/1‑138 (MQ=255)                                                     cTTTCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCacta                                                                            <  4:259614/138‑1 (MQ=255)                                                       ttCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATAcc                                                                                                               >  2:316773/1‑101 (MQ=255)                                                       ttCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATAcc                                                                                                               <  1:316773/101‑1 (MQ=255)                                                       ttCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCacta                                                                            >  6:245577/1‑136 (MQ=255)                                                       ttCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCacta                                                                            <  5:245577/136‑1 (MQ=255)                                                        tCGAAATAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCactact                                                                          <  6:276246/137‑1 (MQ=255)                                                          gaaaTAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTAt                                                                      <  3:52142/136‑1 (MQ=255)                                                           aaaTAGTGGCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATc                                                                     <  5:272245/137‑1 (MQ=255)                                                               agtggCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         <  3:397199/83‑1 (MQ=255)                                                               agtggCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCt                                                                                                                         >  4:397199/1‑83 (MQ=255)                                                                gtggCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGc                                                                 <  4:442039/135‑1 (MQ=255)                                                                 tggCGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGACACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCa                                                                >  3:239955/3‑138 (MQ=255)                                                                    cGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGt                                                            <  5:126017/139‑1 (MQ=255)                                                                    cGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGt                                                            <  3:45283/139‑1 (MQ=255)                                                                    cGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGt                                                            <  8:23643/139‑1 (MQ=255)                                                                    cGGCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGt                                                            <  7:193238/139‑1 (MQ=255)                                                                    cGGCATGAAGAGGGGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGt                                                            <  4:280094/139‑1 (MQ=255)                                                                     ggCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTg                                                           <  3:437794/139‑1 (MQ=255)                                                                      gCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTacaca                                                                                    <  3:199563/113‑1 (MQ=255)                                                                      gCATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTacaca                                                                                    >  4:199563/1‑113 (MQ=255)                                                                       cATGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGcc                                                         >  7:216348/1‑139 (MQ=255)                                                                         tGAAGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACCTCGTCATTTAAAAATGCCTGAAACCTACCCCTCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCtt                                                       >  4:153750/1‑139 (MQ=255)                                                                           aaGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTacaca                                                                                    <  1:59639/108‑1 (MQ=255)                                                                           aaGAGGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTacaca                                                                                    >  2:59639/1‑108 (MQ=255)                                                                            agagGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCaa                                                    <  5:396254/139‑1 (MQ=255)                                                                            agagGTGTATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCaa                                                    <  4:171551/139‑1 (MQ=255)                                                                                   tATCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGttt                                             >  8:140867/1‑139 (MQ=255)                                                                                     tCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCa                                                                               >  3:56872/1‑103 (MQ=255)                                                                                     tCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCa                                                                               <  4:56872/103‑1 (MQ=255)                                                                                     tCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTc                                            <  8:216348/138‑1 (MQ=255)                                                                                     tCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTc                                            >  5:328551/1‑138 (MQ=255)                                                                                     tCAATTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTc                                            >  1:158267/1‑138 (MQ=255)                                                                                       aaTTCCTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTCaac                                         <  3:18022/139‑3 (MQ=255)                                                                                            cTTTTCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTCAACTGAt                                     <  2:139568/138‑1 (MQ=255)                                                                                               ttCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCaa                                                    >  2:70789/1‑120 (MQ=255)                                                                                               ttCCTGCTGCTTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCaa                                                    <  1:70789/120‑1 (MQ=255)                                                                                                       gctTCAGCGGCGATCAGTTCAACATCTTCATTTAAAAATTCCTGAAACATACCCATCGCATTGCTtcctccgc                                                                                           <  2:325055/73‑3 (MQ=255)                                                                                                       gctTCAGCGCCGATCAGTTCAACATCTTCATTTAAAAATTCCTGAAACATACCCATCGCATTGCTtcctccgc                                                                                           >  1:325055/1‑71 (MQ=255)                                                                                                            aGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCaa                                                    <  3:425810/107‑1 (MQ=255)                                                                                                            aGCGCCGATCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCaa                                                    >  4:425810/1‑107 (MQ=255)                                                                                                                   aTCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCAttt             >  5:216926/1‑139 (MQ=255)                                                                                                                   aTCAGTTCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCAttt             >  4:54979/1‑139 (MQ=255)                                                                                                                        ttCAACATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCACTCCGATCAttatttg         >  3:1893/1‑133 (MQ=255)                                                                                                                           aaCATCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAAt     <  8:52458/139‑1 (MQ=255)                                                                                                                              aTCTTCATTTAAAAATGCCTGAAACATACCCATCGCATTGCTTCCTCCGCCTACACATGCCACTACTTTATCAGGCATAGTGCCTTCAATACGTTTCAACTGATCCTTCGCTTCCTCTCCGATCATTTTTTGAAATtca  <  6:405140/139‑2 (MQ=255)                                                                                                                                    |                                                                                                                                     AACTCATCTTGAATCAGATAAGTCAATGAACCGTGAATAACGCCTACGGTTCCTTTCGACATAGTAGCGGCATGAAGAGGAGTATCAATTCCTTTTCCGGCTGCTTCAGCGCCGATCAGTTCAACATCTTCGTCTAAAAACGCCTGAAACATGCCTATCGCATTGCTTCCTCCACCTACACACGCCACTACTTTATCAGGCAGAGTGCCTTCAATGTGTTTCAGCTGATCCTTCGCTTCCTCTCCAATCATTTTCTGAAATTCT  >  CP006881/2190491‑2190754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑